Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein(Il16) (Active)

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Code CSB-AP003381MO
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T lymphocytes is in a concentration range of 1.0-100 ng/ml.
Target Names Il16
Uniprot No. O54824
Research Area Immunology
Alternative Names Il16; Pro-interleukin-16 [Cleaved into: Interleukin-16; IL-16; Lymphocyte chemoattractant factor; LCF)]
Species Mus musculus (Mouse)
Source E.coli
Expression Region 1205-1322aa
Complete Sequence SAASASAASD ISVESKEATV CTVTLEKTSA GLGFSLEGGK GSLHGDKPLT INRIFKGTEQ GEMVQPGDEI LQLAGTAVQG LTRFEAWNVI KALPDGPVTI VIRRTSLQCK QTTASADS
Mol. Weight 12.2 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.; Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.; Isoform 2 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
Gene References into Functions
  1. The production of IL-16 contributed to lung damage as neutralization of IL-16 enhanced S. aureus clearance and resulted in diminished lung pathology in S. aureus pneumonia. PMID: 24736233
  2. Data indicate that knockdown of translocator protein TSPO by siRNAhad showed no effect on the productions of TNF-alpha, IL-1beta and IL-6 in lipopolysaccharide (LPS)-stimulated BV-2 microglia cells. PMID: 25200148
  3. Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction. PMID: 23894370
  4. Data show overexpression of pro-IL-16 impaired resting B cell proliferation mediated through regulating nuclear factor (NF)-kappaB activation, and siRNA knock-down of pro-IL-16 expression decreased the level of p27(kip) and increased the level of Skp2. PMID: 23297678
  5. We determined that ovalbumin (OVA)-sensitized mice have an increase in systemic tumor necrosis factor-alpha levels, and that serum or BAL fluid stimulation of bronchial epithelial cells results in production of IL-16 PMID: 12594062
  6. IL-16 induces a greater migratory response in the Th1 cell subset than in the Th2 subset. PMID: 14607889
  7. interleukin-16 and its precursor have roles in T lymphocyte activation and growth [review] PMID: 15253385
  8. IL-16 precursor mRNA and protein expression are dynamically regulated during CD4+ T cell activation by a calcineurin-dependent mechanism; pro-IL-16 may influence T cell cycle regulation, although not in a dominant manner. PMID: 15728482
  9. Caspase-3 mediated production of IL-16 by infiltrating CD4+ T cells, contributes to ongoing neuroinflammation by chemoattraction of additional waves of CD4+ T cells. PMID: 16271292
  10. After femoral artery ligation, CD8+ T cells infiltrate the site of collateral vessel growth and recruit CD4+ mononuclear cells through the expression of IL-16 PMID: 16380545
  11. Inactivation of IL-16 by antibody therapy or IL-16 deficiency prevented ischemia-reperfusion injury of the kidney, as shown by reduced levels of serum creatinine or blood urea nitrogen. PMID: 18004294
  12. Generation of bleomycin-induced lung fibrosis is independent of IL-16 PMID: 19232499
  13. CD4-positive T cells are specifically recruited to the lung in a dendritic cell-independent but IL-16-dependent process and diminish neutrophil recruitment through increased IL-10 production. PMID: 19641139

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Subcellular Location Secreted.; [Isoform 1]: Cytoplasm. Note=Colocalizes with GRIN2C in neuronal cell bodies and neurites.; [Isoform 2]: Cytoplasm. Nucleus.
Tissue Specificity Isoform 1 is expressed in neurons of the cerebellum and hippocampus. Isoform 2 is expressed in thymus, spleen and lung.
Database Links

KEGG: mmu:16170

STRING: 10090.ENSMUSP00000001792

UniGene: Mm.10137

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