CANX Monoclonal Antibody

Code CSB-MA004485A0m
Size US$350 How to order?
Image
  • Western Blot
    Positive WB detected in: MCF7 whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, PC-3 whole cell lysate, SY5Y whole cell lysate
    All lanes: CANX antibody at 1:2000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 68, 72, 56 kDa
    Observed band size: 90 KDa
    Exposure time:5min
  • Overlay Peak curve showing Hela cells stained with CSB-MA004485A0m (red line) at 1:80. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Full Product Name Mouse anti-Homo sapiens (Human) CANX Monoclonal antibody
Uniprot No. P27824
Target Names CANX
Alternative Names Calnexin antibody; CALX_HUMAN antibody; CANX antibody; CNX antibody; FLJ26570 antibody; Histocompatibility complex class I antigen binding protein p88 antibody; IP90 antibody; Major histocompatibility complex class I antigen-binding protein p88 antibody; p90 antibody
Raised in Mouse
Species Reactivity Human
Immunogen Recombinant Human Calnexin protein (1-482AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG2a
Clone No. 4G3H3
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB WB:1:1000-1:5000
FC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Gene References into Functions
  1. In summary, ER retention of pathogenic VLDLR mutants involves binding to calnexin, elevated endoplasmic reticulum stress, and delayed degradation which is dependent on SEL1L. PMID: 29371607
  2. Increased tumour protein levels of calnexin may be of prognostic significance in colorectal cancer, and calnexin may represent a potential target for future therapies. PMID: 27369741
  3. FUNDC1 integrates mitochondrial fission and mitophagy at the interface of the endoplasmic reticulum-mitochondrial contact site by working in concert with DRP1 and calnexin under hypoxic conditions in mammalian cells. PMID: 27145933
  4. Results of further analyses by using a CNX mutant imply that ERp29 and ERp57 recognize the same domain of CNX, whereas the mode of interaction with CNX might be somewhat different between them. PMID: 28456374
  5. Inhibit interaction between HIV-1 Nef and Calnexin to reverse HIV-induced lipid accumulation and prevent atherosclerosis. PMID: 27470515
  6. Endogenous NOX4 forms macromolecular complexes with calnexin, which are needed for the proper maturation, processing, and function of NOX4 in the endoplasmic reticulum. PMID: 26861875
  7. To study principles underlying dynamics and regulation of palmitoylation, the ER chaperone calnexin, which requires dual palmitoylation for function, was investigated. PMID: 26900856
  8. These observations point at a previously unrecognized contribution of calnexin to the retention of NMT1 at the ER membrane. PMID: 26603938
  9. Charcot-Marie-Tooth disease-related PMP22 is trapped in the endoplasmic reticulum by calnexin-dependent retention and Rer1-mediated early Golgi retrieval systems and partly degraded by the Hrd1-mediated endoplasmic reticulum-associated degradation system. PMID: 25385046
  10. Data indicate that protein tyrosine phosphatase 1B (PTP1B) association with calnexin is ubiquitin conjugating enzyme 9 (UBC9)-dependent. PMID: 25586181
  11. H-ERG trafficking was impaired by H2O2 after 48 h treatment, accompanied by reciprocal changes of expression between miR-17-5p seed miRNAs and several chaperones (Hsp70, Hsc70, CANX, and Golga20) PMID: 24386440
  12. Nef regulates the activity of calnexin to stimulate its interaction with gp160 at the expense of ABCA1 PMID: 25170080
  13. The impact of CANX genetic variants to heart rate was discovered. PMID: 24680774
  14. Soluble calnexin may fulfill functions similar to calreticulin. PMID: 24056258
  15. calnexin tunes the cellular responses to epidermal growth factor receptor in a manner that depends on the health status of the endoplasmic reticulum PMID: 23932718
  16. These findings demonstrated that calnexin strictly monitors the maturation of S protein by its direct binding, resulting in conferring infectivity on severe acute respiratory syndrome coronavirus. PMID: 22915798
  17. The data suggest that patients with low or defective TAP1 or calnexin in primary breast cancers may be at higher risks for developing brain metastasis due to the defects in T cell-based immunosurveillance. PMID: 22065046
  18. cysteine residues within calnexin are important for the structure and function of calnexin. PMID: 21842374
  19. The findings showed that calnexin is a stable component of the ribosome-translocon complex in a manner that is exquisitely dependent on calnexin's palmitoylation status. PMID: 22314232
  20. HLA-I, TAP1, CNX, LMP7, Erp57, Tapasin and ERAP1 were down-regulated in 68%, 44%, 48%, 40%, 52%, 32% and 20% of esophageal squamous cell carcinoma lesions then, respectively. PMID: 21362330
  21. Nixin/ZNRF4 to be central for the regulation of calnexin turnover. PMID: 21205830
  22. Results reveal the P-domain functions as a unique protein-protein interaction domain and implicate a peptidyl prolyl isomerase as a new element in the calnexin cycle. PMID: 20801878
  23. calnexin could bind PrP both in vitro and in vivo; calnexin prevents caspase-3-mediated cytotoxicity induced by PrP PMID: 20506117
  24. Transmembrane segments prevent surface expression of sodium channel Nav1.8 and promote calnexin-dependent channel degradation PMID: 20720009
  25. human delta opioid receptor (hdeltaOR) exists in a ternary complex with SERCA2b and the ER molecular chaperone calnexin PMID: 20528919
  26. Env interacts with intracellular CNX and extracellular PDI via discrete, largely nonoverlapping, regions. PMID: 20202930
  27. Results show that the phosphorylation of calnexin is linked to the efficiency of secretion of the cargo glycoprotein, in this case, alpha1-antitrypsin. PMID: 19815548
  28. Data show that demonstrate that Cnx preferentially associates with misfolded mutant opsins associated with retinitis pigmentosa. PMID: 19801547
  29. EDEM appeared to function in the ERAD (endoplasmic reticulum-associated degradation)pathway by accepting substrates from calnexin. PMID: 12610305
  30. EDEM was shown to extract misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle PMID: 12610306
  31. Data show that calnexin associates with newly synthesized proteolipid protein (PLP) molecules, binding stably to misfolded PLP. PMID: 12805210
  32. calnexin-downregulation may contribute to the metastatic phenotype of melanoma cells in vivo PMID: 14732231
  33. Calnexin provides long-term protection of wild-type Shaker protein from ER-associated degradation. PMID: 15161937
  34. the contribution of both the b and b' domains to the binding with CNX and calreticulin was revealed PMID: 15236594
  35. results support emerging models for a glycan-independent chaperone role for calnexin and for the mechanism of retention of misfolded membrane proteins in the endoplasmic reticulum PMID: 15537650
  36. Calnexin decreases with aging and might contribute to a cytoprotection in a variety of human age-related diseases. PMID: 15557823
  37. Data show that the major degradation pathway of the cystic fibrosis transmembrane conductance regulator with F508 deletion from the endoplasmic reticulum is independent of calnexin. PMID: 15923638
  38. Calnexin associates with the neonatal Fc receptor for IgG (FcRn) heavy chain before it associates noncovalently with beta 2-microglobulin. PMID: 16002696
  39. Polypeptide substrate recognition by CANX requires specific conformations of the CANX protein. PMID: 16061483
  40. We characterized a molecular mechanism by which calnexin regulates ER-stress-mediated apoptosis in a manner independent of its chaperone functions but dependent of its binding to Bap31. PMID: 16858427
  41. Here, we have observed that NCT N-linked oligosaccharides mediated specific interactions with the secretory pathway lectins calnexin and ERGIC-53 PMID: 16938437
  42. proportion of the human and the rat WT gonadotropin-releasing hormone receptor appears to be retained in the endoplasmic reticulum by calnexin, an effect that decreases GnRHR signaling capacity PMID: 17170088
  43. These results suggest that MCF-7 resistance to endoplasmic reticulum stress-induced apoptosis is partially mediated by the expression level of calnexin which in turn controls its sub-cellular localization, and its association with Bap31. PMID: 17203246
  44. D1 and D2 dopamine receptor expression is regulated by direct interaction with the chaperone protein calnexin PMID: 17395585
  45. Endoplasmic reticulum chaperones stabilize nicotinic receptor subunits and regulate receptor assembly. PMID: 17728248
  46. Interaction with calnexin led to accumulation of GAT1 in concentric bodies corresponding to previously described multilamellar ER-derived structures. PMID: 18367207
  47. the phosphorylation state of the calnexin cytosolic domain and its interaction with PACS-2 sort the chaperone between domains of the ER and the plasma membrane PMID: 18417615
  48. A dependence on calnexin for proper assembly of CFTR's membrane spanning domains, was identified. PMID: 18716059
  49. ERp57 must be physically associated with the calnexin cycle to catalyze isomerization reactions with most of its substrates PMID: 19054761
  50. alphaIIb interacts with calnexin via its N15-linked glycan, and alphaIIbbeta3 biogenesis is partially controlled by engagement of alphaIIb in the calnexin cycle. PMID: 16304048

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Subcellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome.
Protein Families Calreticulin family
Database Links

HGNC: 1473

OMIM: 114217

KEGG: hsa:821

STRING: 9606.ENSP00000247461

UniGene: Hs.567968

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