GFAP Monoclonal Antibody

Datasheet
Code CSB-MA009369A0m
See More Details Free Antibody trial simple
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Mouse brain tissue, Rat brain tissue
    All lanes: GFAP antibody at 2.7µg/ml
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 50, 51 kDa
    Observed band size: 50 kDa

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-MA009369A0m at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human glioma using CSB-MA009369A0m at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA009369A0m at dilution of 1:100

  • Immunofluorescent analysis of A549 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of SH-SY5Y cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of U251 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of U87 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Overlay histogram showing SH-SY5Y cells stained with CSB-MA009369A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG2b (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Product Details

Uniprot No. P14136
Alternative Names Glial fibrillary acidic protein, GFAP
Raised in Mouse
Species Reactivity Human, Mouse, Rat
Immunogen Recombinant Human GFAP protein (292-432AA)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG2b
Clone No. 1C91F1
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:500
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Q&A and Customer Reviews

 Customer Reviews
  • Tested application: IF
    Test sample: U87
    Primary antibody dilution ratio: 1:80
    Review: GFAP was expressed in cytoplasm and membrane.

Target Data

Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Involvement in disease Alexander disease (ALXDRD)
Subcellular Location Cytoplasm
Protein Families Intermediate filament family
Tissue Specificity Expressed in cells lacking fibronectin.
Database Links

HGNC: 4235

OMIM: 137780

KEGG: hsa:2670

STRING: 9606.ENSP00000253408

UniGene: Hs.514227

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