Human glial fibrillary acidic protein,GFAP ELISA Kit

Code CSB-E08601h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P14136
Lead Time 3-5 working days
Abbreviation GFAP
Target Name glial fibrillary acidic protein
Alias FLJ45472,
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
HGNC 4235
RGD 2679
MGI 95697
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %100
Range %97-103
1:2Average %101
Range %98-104
1:4Average %95
Range %90-100
1:8Average %92
Range %89-96
Recovery
The recovery of human GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-104
EDTA plasma (n=4)9387-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.437 2.578 2.508 2.412
202.241 2.256 2.249 2.153
101.778 1.790 1.784 1.688
51.205 1.224 1.215 1.119
2.50.712 0.738 0.725 0.629
1.250.504 0.529 0.517 0.421
0.6250.248 0.267 0.258 0.162
00.095 0.097 0.096  
References
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Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Involvement in disease Alexander disease (ALXDRD)
Subcellular Location Cytoplasm
Protein Families Intermediate filament family
Tissue Specificity Expressed in cells lacking fibronectin.
Database Links

HGNC: 4235

OMIM: 137780

KEGG: hsa:2670

STRING: 9606.ENSP00000253408

UniGene: Hs.514227

Pathway Jak-STAT signaling pathway

Q&A and Customer Reviews

Q&A
Q:

I am interested in conducting a research on “ serum GFAP level and it’s correlation with histopathological grading of solitary supra Tentorial space occupying lesion.I would be grateful if you you could let me know wheather you would be able to supply the kit .if you do then how many kit do I need to cover sample of 50-60 cases?

A:
Thanks for your inquiry.
Your research purpose is feasible. We have this kit available: Human glial fibrillary acidic protein,GFAP ELISA Kit CSB-E08601h. For a 96T kit, the standard is S0-S7. It can test 88 samples. If you run duplicated wells, it can test 44 samples.
Customer Reviews

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