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Conversion result will appear
here...
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Conversion Info
Sequence Type: DNA
Conversion Type: Reverse Complement Sequence
Sequence Analysis
Sequence analysis will display here after
conversion...
Base Pairing Rules
DNA Base Pairing
A
Adenine
T
Thymine
C
Cytosine
G
Guanine
RNA Base Pairing
A
Adenine
U
Uracil
C
Cytosine
G
Guanine
About the Sequence Conversion Tool
Learn about the principles of nucleic acid sequence reverse complement conversion and the features of this tool
Conversion Principles
Reverse complement conversion of nucleic acid sequences is a fundamental operation in molecular biology. The reverse sequence is the original sequence in reverse order. The complementary sequence is generated based on base pairing rules, and the reverse complement sequence is the complementary sequence in reverse order.
Features
Supports three conversion modes for DNA and RNA sequences, automatically processes common impurity characters in sequences, displays real-time sequence analysis results, supports result copying and downloading, and provides sample sequences for quick testing.
Applications
Suitable for PCR primer design, gene cloning, sequence alignment, molecular biology experiment design and other scenarios, helping researchers quickly generate and analyze the reverse complement strands of nucleic acid sequences.
How to Use
Learn how to use this tool to perform reverse complement conversion of nucleic acid sequences
1 Enter Nucleic Acid Sequence
Paste or type your nucleic acid sequence into the input box. The sequence can contain standard DNA bases (A, T, C, G) or RNA bases (A, U, C, G), case-insensitive. Spaces, numbers, and other non-base characters are allowed and will be automatically filtered.
Valid sequence examples:
ATGCCTAGCTAGCTAGCTAG
augccuagcuagcuagcuag
ATGC (123) TAGC
2 Select Conversion Options
- Select sequence type: DNA or RNA
- Select conversion type: Reverse Sequence, Complementary Sequence, or Reverse Complement Sequence
- Choose whether to auto-convert and whether to output in uppercase
3 View Conversion Results
The tool will generate the corresponding converted sequence based on your selected options and display it in the output area. Sequence analysis information, including sequence length, base composition, and GC content, is also provided.
Conversion result example:
CTAGCTAGCTAGCTAGGCAT
4 Save or Analyze Results
- Click the "Copy" button to copy the converted sequence
- Click the "Download" button to save the sequence as a text file
- Click the "Analyze" button for more detailed sequence analysis
📺Tutorial Video
To help you make the most of the Nucleotide Reverse Complement Tool, we’ve prepared a short demonstration video. Watch it below to see how to quickly generate reverse complement sequences for DNA or RNA, and learn tips for accurate sequence analysis.
