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| Code | CSB-MP004939HU |
| Size | US$348 How to order? |
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| Description |
The DNA fragment encoding the human CD46 (Cys35-Tyr328) was expressed with a C-terminal hFc-tag in the mammalian cells. The expressed protein is the recombinant human CD46 protein with low endotoxin (< 1.0 EU/µg protein, LAL method) and high purity (> 92%, SDS-PAGE). This CD46 protein displayed a molecular weight of approximately 90 kDa on the gel due to glycosylation. It is biologically active. In the functional ELISA, this CD46 protein can bind to the anti-CD46 antibody with an EC50 of 0.8333-1.054 ng/ml. This recombinant CD46 protein is in stock now. CD46 is ubiquitously expressed, alternatively spliced complement regulatory protein. It acts as a cofactor for the serine protease factor I to mediate the interaction of C3b and C4b deposited on host cells, thus protecting host cells from injury. CD46 also modulates adaptive immunity and participates in human infectious and Th1 biology. Increased expression of CD46 has been found in many types of cancer. |
| Purity | Greater than 92% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054 ng/ml. |
| Target Names | CD46 |
| Uniprot No. | P15529 |
| Research Area | Cancer |
| Alternative Names | CD46; MCP; MIC10; Membrane cofactor protein; TLX; Trophoblast leukocyte common antigen; CD antigen CD46 |
| Molecular Characterization |  |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 35-328aa |
| Target Protein Sequence | CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY |
| Mol. Weight | 61.6 kDa |
| Protein Length | Partial |
| Tag Info |
C-terminal hFc-tagged |
| Form | Liquid or Lyophilized powder |
| Buffer | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Basically, we can dispatch the products out in 1-3
working days after receiving your orders. Delivery time may differ from different purchasing way or
location, please kindly consult your local distributors for specific delivery time. Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.; (Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.; (Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.; (Microbial infection) Acts as a receptor for cultured Measles virus.; (Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.; (Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes.
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| Gene References into Functions |
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| Involvement in disease | Hemolytic uremic syndrome atypical 2 (AHUS2) |
| Subcellular Location | Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane; Single-pass type I membrane protein. Note=Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide. |
| Tissue Specificity | Expressed by all cells except erythrocytes. |
| Database Links |
HGNC: 6953 OMIM: 120920 KEGG: hsa:4179 STRING: 9606.ENSP00000313875 UniGene: Hs.510402 |
