Recombinant Human Membrane cofactor protein(CD46) (Active)

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Code CSB-MP004939HU
Size US$348
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054 ng/ml. Biological Activity Assay
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Product Details

Purity Greater than 92% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054 ng/ml.
Target Names CD46
Uniprot No. P15529
Research Area Cancer
Alternative Names AHUS2; Antigen defined by monoclonal TRA 2 10; Antigen identified by monoclonal TRA 2 10; CD46; CD46 antigen; CD46 antigen complement regulatory protein; CD46 molecule; CD46 molecule complement regulatory protein; Complement membrane cofactor protein; MCP; MCP_HUMAN; Measles virus receptor ; membrane cofactor protein (CD46; trophoblast-lymphocyte cross-reactive antigen); Membrane cofactor protein; MGC26544; MIC10; TLX; TRA2.10; Trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; Trophoblast lymphocyte cross reactive antigen
Molecular Characterization
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 35-328aa
Target Protein Sequence CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY
Mol. Weight 61.6 kDa
Protein Length Partial
Tag Info C-terminal FC-tagged
Form Liquid or Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
Gene References into Functions
  1. Only type-I interferon restricts viral replication of attenuated Measles virus (MV) Schwarz strain in mice, independently of the presence of hCD46 receptor. PMID: 30199752
  2. CD46 levels were positive in 3.3% (n = 3) and negative in 96.7% (n = 90). Conclusion This study does not support the theory of measles virus as the cause of otosclerosis. PMID: 28971731
  3. Findings demonstrate that CD46 is a key inhibitor of complement activation whose downregulation may be involved in the pathogenesis of bullous pemphigoid. PMID: 28273946
  4. these data suggest a possible mechanism underlying the regulatory function of CD46 on T cells. PMID: 29066539
  5. study demonstratede that MMP-9-mediated shedding of CD46 is an integral part of autocrine Th1 regulation and that this pathway is dysregulated in T cells from patients with systemic lupus erythematosus PMID: 28444759
  6. the cell surface expression level of CD46 was markedly higher in patient myeloma cells with 1q gain than in those with normal 1q copy number. PMID: 27841764
  7. Our findings reveal a novel role of CD46 in accelerating inflammatory responses upon meningococcal infection or LPS stimulation by regulating the functional polarization and survival of macrophages. PMID: 27794168
  8. study reveals common CD46 and IFI44L SNPs associated with measles-specific humoral immunity, and highlights the importance of alternative splicing/virus cellular receptor isoform usage as a mechanism explaining inter-individual variation in immune response after live measles vaccine PMID: 28289848
  9. study showed no genotype differences in CD46 IVS1-1724 C>G Single nucleotide polymorphis (SNP) between Recurrent Spontaneous Abortion and fertile women PMID: 27921411
  10. Isoforms of CD46 impact on HHV-6B infection and thereby in part explain the distinct tropism of HHV-6AGS and HHV-6BPL1. PMID: 28056415
  11. This study suggests that the promoter polymorphism (rs2796267, -496 A/G) CD46 gene may be related to susceptibility of acute renal allograft rejection in Korean kidney transplantation recipients. PMID: 27234742
  12. provides insight into CD46 alternative splicing regulation with implications for its function in the immune system and for genetic disease PMID: 27226545
  13. CD46 controls both Th1 activation and regulation via a miR-150-dependent mechanism. PMID: 26746193
  14. Data show that CD28 antigen costimulation modulates CD46 antigen surface expression on activated T cells. PMID: 25787182
  15. This review summarizes our current knowledge of disease-associated mutations in this complement inhibitor(CD46). PMID: 26054645
  16. It was not discover any association between A304V or other CD46 SNPs and preeclampsia. PMID: 25710174
  17. gene deficiency results in defective Th1 cell induction due to failure in metabolic reprogramming PMID: 26084023
  18. Each mutant C3b bound MCP at reduced affinity, providing a molecular basis for its reduced cofactor activity in atypical hemolytic uremic syndrome. PMID: 25879158
  19. The mutations in the regulators CFH, CFI and MCP involve loss-of-function, whereas those for C3 involve gain-of-function. PMID: 25188723
  20. Results suggest a possible role for multidrug resistance genes ABCA10 and ABCA6 in follicular lymphoma survival and add to the evidence that genetic variation in CD46 and IL8 may have prognostic implications follicular lymphoma. PMID: 25294155
  21. Expression of membrane complement regulators, CD46, CD55 and CD59, in mesothelial cells of patients on peritoneal dialysis therapy. PMID: 25725314
  22. Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients. PMID: 25733390
  23. Transgenic hCD46/HLA-E expression reduced humoral xenoresponses since all were downregulated during ex vivo xenoperfusion of hCD46/HLA-E double transgenic pig forelimbs with human blood. PMID: 24635052
  24. The expression levels of CD46, CD55, and CD59 were significantly higher in colon cancer tissues compared with the normal adjacent colon tissues. PMID: 24978917
  25. The present study suggests that CD46 plays an important role in hepatocellular carcinoma carcinogenesis by regulating let-7b and miR-17. PMID: 24297460
  26. Data show that calcitriol affects the CD46 pathway, and that it promotes anti-inflammatory responses mediated by CD46. PMID: 23144765
  27. We found that CD46 and CD59 were decreased in retinal pigment epithelium cells in part, by their release in exosomes and apoptotic particles. PMID: 24664706
  28. C3aR and CD46 activation via intrinsic generation of their respective ligands is an integral part of human Th1 (but not Th2) immunity. PMID: 24321396
  29. patients with MCP mutations only have a good long-term prognosis with 15% to 20% disease recurrence after KTx PMID: 24247905
  30. Case Report: patient with atypical haemolytic uremic syndrome with combined membrane cofactor protein CD46 and complement factor I mutations undergoing successful kidney transplantation. PMID: 23519521
  31. Mutations in membrane cofactor protein is associated with end-stage renal disease in a patient with hemolytic uremic syndrome caused by infections by Escherichia coli strains producing Shiga-like toxins. PMID: 23731345
  32. Data show that increased CD35, CD46, CD55 and CD59 on neutrophils and monocytes present potent markers of bacterial infection and viral infection. PMID: 23376460
  33. Thus, while nectin-4 and CD46 interact functionally with the measles virus H protein beta4-beta5 hydrophobic groove, SLAM merely covers it. PMID: 23760251
  34. HCV incorporates selectively CD59, but not CD46 or CD55, in its envelope to gain resistance to CML in serum of infected individuals PMID: 23049856
  35. Hepatitis B virus X protein protects hepatoma and hepatic cells from complement-dependent cytotoxicity by up-regulation of CD46. PMID: 23391762
  36. The alternative pathway of complement may play a role in the pathogenesis of HELLP syndrome. PMID: 22594569
  37. The aim of this study was to determine the transcript and protein levels of complement decay-accelerating factor (DAF) and membrane cofactor protein (MCP) in the placentas of women with acquired and inherited thrombophilia. PMID: 23042280
  38. we found that CD46 regulated the expression of Notch receptors and ligands during T cell activation and that disturbance of the CD46-Notch crosstalk impeded induction of IFN-alpha and switching to IL-10. PMID: 23086448
  39. Data show that mutations in CD46 and CFH did not predispose to systemic lupus erythematosus (SLE) or nephritis but was associated with earlier onset of nephritis. PMID: 22171659
  40. mumps virus (MuV) and vesicular stomatitis virus (VSV) assemble to include CD46 and CD55, two host cell regulators which inhibit propagation of complement pathways through distinct mechanisms. PMID: 22761385
  41. Prostaglandin (PG)E2 has a novel role in CD46 functions, which might at least partly explain the diverse roles of PGE2 in T cell functions. PMID: 22544928
  42. Three CD46 variants (rs7144, rs11118580, and rs2724384) were significantly associated with measles virus-specific IgG levels (P = 0.008, P = 0.026, and P = 0.018, respectively). PMID: 22357652
  43. in systemic lupus erythematosus, the expression of leukocyte MCP at the mRNA level is closely related to disease activity PMID: 22247341
  44. Data suggest that HBXIP upregulates CD46, CD55 and CD59 through p-ERK1/2/NF-kappaB signaling to protect breast cancer from complement-dependent cytotoxicity. PMID: 22293503
  45. The strongest associations replicated in both cohorts were found for four SNPs within CD46 (p-value<10(-3)) and five SNPs within CFH (p-value<5x10(-3)). PMID: 22153652
  46. Subjects previously immunized with measles-mumps-rubella vaccine were genotyped for 66 candidate single nucleotide polymorphisms in the CD46, SLAM and CD209 genes. PMID: 22086389
  47. Mutations in the MCP gene seems to be a common etiology of atypical hemolytic uremic syndrome in Czech patients PMID: 21706448
  48. protein expression is an unfavorable prognostic factor in breast cancer cases PMID: 21617523
  49. Data sshow that variants in SFTPD, CD46 and IL1R1 are associated with IPD in both EA and AA. PMID: 21858107
  50. the A304V mutation in the MCP gene is not strongly associated with RM or FL. PMID: 21840606
  51. The lipopolysaccharide of Treponema denticola and Tannerella forsythia were the most potent for increasing the gene expression of CD55 and CD59, and to a lesser extent CD46, after a 48-h stimulation. PMID: 21545652
  52. CD46 could be associated with the response to interferon-beta therapy in multiple sclerosis. PMID: 21177319
  53. CD46 acts as a molecular rheostat to control human T cell activation through the regulation of processing of its cytoplasmic tails. PMID: 21283821
  54. The simultaneous interaction of Measles virus glycoprotein-pseudotyped Lentivirus with both CD46 and SLAM is required to achieve efficient and stable transduction of B and T cells. PMID: 21450813
  55. Fusobacterium nucleatum induced IL-6, IL-8, and MMP-9 gene expression and protein secretion by epithelial cells through interactions with cell surface CD46. PMID: 20843232
  56. Adenovirus (Ad) 35 vectors are better than Ad5F35 vectors at inducing innate immune responses in murine dendritic cells expressing human CD46 and CD46 transgenic mice, even though both Ad vectors utilize the same receptor. PMID: 20800630
  57. Studies indicate that discovery of genetic abnormalities in complement regulatory genes provide new insights into the influence of each abnormality on the disease outcome and opened new perspectives for disease management. PMID: 20865641
  58. In non-Hodgkin lymphoma patients treated with rituximab plus chemotherapy, expression of CD46 was higher in patients with complete remission than in non-responders. CD59 expression may be related to resistance to rituximab. PMID: 19662541
  59. The absence of CD46H*1 homozygosity was more pronounced in women with recurrent spontaneous abortion in the Indian population. PMID: 20925204
  60. We show here that Adenovirus 11p virions downregulate CD46 on the surface of K562 cells as early as 5min p.i. Specific binding to CD46 by the Ad11p fiber knob was required to mediate downregulation. PMID: 20638094
  61. The induction of expression of CD46 by endothelial cells in dilated cardiomyopathy suggests that viruses targeting these receptors could more easily gain entry to heart cells after intravascular administration. PMID: 19957088
  62. MV-H glycoprotein from an Edmonston MV variant & MV-binding fragment of CD46 receptor were overproduced in mammalian cells & used to crystallize MV-H-CD46 complex. Well diffracting crystals containing 2 complexes in the asymmetric unit were obtained PMID: 20057080
  63. Increased expression levels of these isoforms and special CD46 expression pattern of the human otic capsule might produce modified or pathological intracellular signalization that could create the possibility of persistent measles virus infection. PMID: 19597833
  64. The transplantation of islets from hCD46 pigs resulted in graft survival and insulin-independent normoglycemia in four of five monkeys for the 3 months follow-up of the experiment PMID: 19845582
  65. the lack of cTreg function in optimizing B-cell responses could explain why some CD46-deficient patients develop common variable immunodeficiency PMID: 19784949
  66. Neisseria gonorrhoeae and Neisseria meningitidis, stimulate presenilin/gammaS processing of CD46-cyt1 and CD46-cyt2, a mechanism by which signals are transduced via the cyt1 and cyt2 tails to regulate CD46-dependent cellular responses. PMID: 20018629
  67. Association of factor H autoantibodies with deletions of CFHR1, CFHR3, CFHR4, and with mutations in CFH, CFI, CD46, and C3 in patients with atypical hemolytic uremic syndrome. PMID: 19861685
  68. DAF and MCP regulate cell survival of human cervical cancer cells. PMID: 19878546
  69. CD46 costimulation induces morphological changes in T cells and activates the MAP Kinase Signaling System PMID: 11739493
  70. peripheral blood monocytes treated for 5--8 days with GM-CSF (i.e. mature Mphi) acquired the capacity to assemble CD9, alpha3-beta1 integrin and the tyrosine phosphatase SHP-1 with their CD46 PMID: 11858824
  71. The findings reported here strongly suggest that pilus binding to CD46 is not a simple static process. Rather, they support a model in which pilus interaction with CD46 promotes signaling cascades important for Neisseria infectivity. PMID: 11901164
  72. Production and characterization of a pig line transgenic for human membrane cofactor protein. PMID: 11983016
  73. required for HHV-6A induced fusion from without PMID: 12050388
  74. For the classical pathway, MCP is the cofactor for C4b cleavage (and factor H for C3b cleavage); however, if the alternative pathway mediates C3b deposition, then MCP's cofactor activity is sufficient to restrict complement activation. PMID: 12055245
  75. CD46 plays a role in regulating T cell-induced inflammatory reaction and in fine-tuning the cellular immune response by bridging innate and acquired immunity. PMID: 12055630
  76. CD46 isoforms function as receptors for human herpesvirus 6 and measles virus PMID: 12171934
  77. Co-engagement of CD3 and the complement regulator CD46 in the presence of IL-2 induces a Tr1-specific cytokine phenotype in human CD4+ T cells PMID: 12540904
  78. Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex is a viral ligand for human CD46 PMID: 12663806
  79. CD46 has a role as a receptor for fusion and entry of human herpesvirus 6 into target cells PMID: 12724329
  80. role in meningococcal disease; results suggest that CD46 facilitates pilus-dependent interactions at the epithelial mucosa PMID: 12869763
  81. Adenovirus type 11 uses CD46 as a cellular receptor. PMID: 12915534
  82. elucidation of two pathways for CD46 internalization, which are regulated by the valence of cross-linking of CD46 and which utilize either clathrin-coated pits or pseudopodial extension PMID: 12958316
  83. Mutations predispose to development of familial hemolytic uremic syndrome. PMID: 14566051
  84. Data show that CD46 affords protection from antibody-mediated rejection when mouse anti-pig serum was administered to scid mice bearing grafts from either CD46 transgenic or normal pigs. PMID: 14708531
  85. CD46 is localized to the plasma membrane and may have a role in the development of breast cancer epithelial cells PMID: 14712499
  86. Highly efficient CD46 function, controlling both classical and alternative pathway complement activation, which suggests it might be the complement regulator of choice to protect kidney xenografts from pig to papio. PMID: 14962279
  87. In pigs transgenic for human MCP, the transgene is expressed at similar levels to that in human embryonic neural tissue but at an insufficient level to prevent activation of the complement cascade. PMID: 14962294
  88. serves as an attachment receptor for adenovirus-37 PMID: 15047806
  89. all of the major splice forms of CD46 are predominant and functional binding sites of species B Adenovirus 3 on CD46-expressing rodent and human cells but may not be the sole receptor of species B Ads on human cells PMID: 15078926
  90. Review discussing role of CD46 mutations in predisposition to hemolytic uremic syndrome. PMID: 15121049
  91. Significant difference in intensity of the staining of CD55 and CD46 among cells in various layers of normal esophageal mucosa and esophageal carcinoma cells, but not in the staining of CD59. PMID: 15151618
  92. CD46 costimulation promotes a Th1-biased response in human CD4+ T lymphocytes. CD46 enhanced TCR/CD3-induced tyrosine phosphorylation of CD3zeta and ZAP-70. PMID: 15307176
  93. functional activity of CD46 is not restricted to the tumor cell membranes but can be liberated in vesicles and by a metalloproteinase. PMID: 15307194
  94. binding site with measles virus hemagglutinin: attachment sites for MV receptors SLAM and CD46 overlap on the globular head PMID: 15308701
  95. Expression of CD46 was not downregulated by the infection of measles virus strain S191 both on mRNA level and cellular surface protein level. PMID: 15336534
  96. High levels of CD46 is associated with breast cancer PMID: 15378282
  97. Does not provide additional bbenefit in preventing graft rejection in transgenic pig-baboon heart transplantation. PMID: 15385816
  98. there are naturally occurring susceptibility factors in CFH and MCP for the development of atypical haemolytic-uraemic syndrome PMID: 15784724
  99. In transgenic pigs, has no effect on endogenous retrovirus. PMID: 15808690
  100. co-purification of sCD46 and HHV-6 DNA from multiple sclerosis sera indicates that HHV-6 is tightly connected to its receptor, CD46, in the serum of multiple sclerosis patients PMID: 15845255

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Involvement in disease Hemolytic uremic syndrome atypical 2 (AHUS2)
Subcellular Location Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane, Single-pass type I membrane protein
Tissue Specificity Expressed by all cells except erythrocytes.
Database Links

HGNC: 6953

OMIM: 120920

KEGG: hsa:4179

STRING: 9606.ENSP00000313875

UniGene: Hs.510402

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