Purity
Greater than 90% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054 ng/ml.
Alternative Names
CD46; MCP; MIC10; Membrane cofactor protein; TLX; Trophoblast leukocyte common antigen; CD antigen CD46
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
35-328aa
Target Protein Sequence
CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY
Tag Info
C-terminal hFc-tagged
Form
Liquid or Lyophilized powder
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3
working days after receiving your orders. Delivery time may differ from different purchasing way or
location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with
normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The DNA fragment encoding the human CD46 (Cys35-Tyr328) was expressed with a C-terminal hFc-tag in the mammalian cells. The expressed protein is the recombinant human CD46 protein with low endotoxin (< 1.0 EU/µg protein, LAL method) and high purity (> 90%, SDS-PAGE). This CD46 protein displayed a molecular weight of approximately 90 kDa on the gel due to glycosylation. It is biologically active. In the functional ELISA, this CD46 protein can bind to the anti-CD46 antibody with an EC50 of 0.8333-1.054 ng/ml. This recombinant CD46 protein is in stock now.
CD46 is ubiquitously expressed, alternatively spliced complement regulatory protein. It acts as a cofactor for the serine protease factor I to mediate the interaction of C3b and C4b deposited on host cells, thus protecting host cells from injury. CD46 also modulates adaptive immunity and participates in human infectious and Th1 biology. Increased expression of CD46 has been found in many types of cancer.