Name: Recombinant Human Membrane cofactor protein(CD46) (Active)
Brand: CUSABIO
SKU: CSB-MP004939HU

Product Details

Description	The DNA fragment encoding the human CD46 (Cys35-Tyr328) was expressed with a C-terminal hFc-tag in the mammalian cells. The expressed protein is the recombinant human CD46 protein with low endotoxin (< 1.0 EU/µg protein, LAL method) and high purity (> 92%, SDS-PAGE). This CD46 protein displayed a molecular weight of approximately 90 kDa on the gel due to glycosylation. It is biologically active. In the functional ELISA, this CD46 protein can bind to the anti-CD46 antibody with an EC₅₀ of 0.8333-1.054 ng/ml. This recombinant CD46 protein is in stock now. CD46 is ubiquitously expressed, alternatively spliced complement regulatory protein. It acts as a cofactor for the serine protease factor I to mediate the interaction of C3b and C4b deposited on host cells, thus protecting host cells from injury. CD46 also modulates adaptive immunity and participates in human infectious and Th1 biology. Increased expression of CD46 has been found in many types of cancer.
Purity	Greater than 92% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054 ng/ml.
Target Names	CD46
Uniprot No.	P15529
Research Area	Cancer
Alternative Names	AHUS2; Antigen defined by monoclonal TRA 2 10; Antigen identified by monoclonal TRA 2 10; CD46; CD46 antigen; CD46 antigen complement regulatory protein; CD46 molecule; CD46 molecule complement regulatory protein; Complement membrane cofactor protein; MCP; MCP_HUMAN; Measles virus receptor ; membrane cofactor protein (CD46; trophoblast-lymphocyte cross-reactive antigen); Membrane cofactor protein; MGC26544; MIC10; TLX; TRA2.10; Trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; Trophoblast lymphocyte cross reactive antigen
Molecular Characterization
Species	Homo sapiens (Human)
Source	Mammalian cell
Expression Region	35-328aa
Target Protein Sequence	CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY
Mol. Weight	61.6 kDa
Protein Length	Partial
Tag Info	C-terminal hFc-tagged
Form	Liquid or Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Background

Function (From Uniprot)	Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
Gene References into Functions	Only type-I interferon restricts viral replication of attenuated Measles virus (MV) Schwarz strain in mice, independently of the presence of hCD46 receptor. PMID: 30199752 CD46 levels were positive in 3.3% (n = 3) and negative in 96.7% (n = 90). Conclusion This study does not support the theory of measles virus as the cause of otosclerosis. PMID: 28971731 Findings demonstrate that CD46 is a key inhibitor of complement activation whose downregulation may be involved in the pathogenesis of bullous pemphigoid. PMID: 28273946 these data suggest a possible mechanism underlying the regulatory function of CD46 on T cells. PMID: 29066539 study demonstratede that MMP-9-mediated shedding of CD46 is an integral part of autocrine Th1 regulation and that this pathway is dysregulated in T cells from patients with systemic lupus erythematosus PMID: 28444759 the cell surface expression level of CD46 was markedly higher in patient myeloma cells with 1q gain than in those with normal 1q copy number. PMID: 27841764 Our findings reveal a novel role of CD46 in accelerating inflammatory responses upon meningococcal infection or LPS stimulation by regulating the functional polarization and survival of macrophages. PMID: 27794168 study reveals common CD46 and IFI44L SNPs associated with measles-specific humoral immunity, and highlights the importance of alternative splicing/virus cellular receptor isoform usage as a mechanism explaining inter-individual variation in immune response after live measles vaccine PMID: 28289848 study showed no genotype differences in CD46 IVS1-1724 C>G Single nucleotide polymorphis (SNP) between Recurrent Spontaneous Abortion and fertile women PMID: 27921411 Isoforms of CD46 impact on HHV-6B infection and thereby in part explain the distinct tropism of HHV-6AGS and HHV-6BPL1. PMID: 28056415 This study suggests that the promoter polymorphism (rs2796267, -496 A/G) CD46 gene may be related to susceptibility of acute renal allograft rejection in Korean kidney transplantation recipients. PMID: 27234742 provides insight into CD46 alternative splicing regulation with implications for its function in the immune system and for genetic disease PMID: 27226545 CD46 controls both Th1 activation and regulation via a miR-150-dependent mechanism. PMID: 26746193 Data show that CD28 antigen costimulation modulates CD46 antigen surface expression on activated T cells. PMID: 25787182 This review summarizes our current knowledge of disease-associated mutations in this complement inhibitor(CD46). PMID: 26054645 It was not discover any association between A304V or other CD46 SNPs and preeclampsia. PMID: 25710174 gene deficiency results in defective Th1 cell induction due to failure in metabolic reprogramming PMID: 26084023 Each mutant C3b bound MCP at reduced affinity, providing a molecular basis for its reduced cofactor activity in atypical hemolytic uremic syndrome. PMID: 25879158 The mutations in the regulators CFH, CFI and MCP involve loss-of-function, whereas those for C3 involve gain-of-function. PMID: 25188723 Results suggest a possible role for multidrug resistance genes ABCA10 and ABCA6 in follicular lymphoma survival and add to the evidence that genetic variation in CD46 and IL8 may have prognostic implications follicular lymphoma. PMID: 25294155 Expression of membrane complement regulators, CD46, CD55 and CD59, in mesothelial cells of patients on peritoneal dialysis therapy. PMID: 25725314 Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients. PMID: 25733390 Transgenic hCD46/HLA-E expression reduced humoral xenoresponses since all were downregulated during ex vivo xenoperfusion of hCD46/HLA-E double transgenic pig forelimbs with human blood. PMID: 24635052 The expression levels of CD46, CD55, and CD59 were significantly higher in colon cancer tissues compared with the normal adjacent colon tissues. PMID: 24978917 The present study suggests that CD46 plays an important role in hepatocellular carcinoma carcinogenesis by regulating let-7b and miR-17. PMID: 24297460 Data show that calcitriol affects the CD46 pathway, and that it promotes anti-inflammatory responses mediated by CD46. PMID: 23144765 We found that CD46 and CD59 were decreased in retinal pigment epithelium cells in part, by their release in exosomes and apoptotic particles. PMID: 24664706 C3aR and CD46 activation via intrinsic generation of their respective ligands is an integral part of human Th1 (but not Th2) immunity. PMID: 24321396 patients with MCP mutations only have a good long-term prognosis with 15% to 20% disease recurrence after KTx PMID: 24247905 Case Report: patient with atypical haemolytic uremic syndrome with combined membrane cofactor protein CD46 and complement factor I mutations undergoing successful kidney transplantation. PMID: 23519521 Mutations in membrane cofactor protein is associated with end-stage renal disease in a patient with hemolytic uremic syndrome caused by infections by Escherichia coli strains producing Shiga-like toxins. PMID: 23731345 Data show that increased CD35, CD46, CD55 and CD59 on neutrophils and monocytes present potent markers of bacterial infection and viral infection. PMID: 23376460 Thus, while nectin-4 and CD46 interact functionally with the measles virus H protein beta4-beta5 hydrophobic groove, SLAM merely covers it. PMID: 23760251 HCV incorporates selectively CD59, but not CD46 or CD55, in its envelope to gain resistance to CML in serum of infected individuals PMID: 23049856 Hepatitis B virus X protein protects hepatoma and hepatic cells from complement-dependent cytotoxicity by up-regulation of CD46. PMID: 23391762 The alternative pathway of complement may play a role in the pathogenesis of HELLP syndrome. PMID: 22594569 The aim of this study was to determine the transcript and protein levels of complement decay-accelerating factor (DAF) and membrane cofactor protein (MCP) in the placentas of women with acquired and inherited thrombophilia. PMID: 23042280 we found that CD46 regulated the expression of Notch receptors and ligands during T cell activation and that disturbance of the CD46-Notch crosstalk impeded induction of IFN-alpha and switching to IL-10. PMID: 23086448 Data show that mutations in CD46 and CFH did not predispose to systemic lupus erythematosus (SLE) or nephritis but was associated with earlier onset of nephritis. PMID: 22171659 mumps virus (MuV) and vesicular stomatitis virus (VSV) assemble to include CD46 and CD55, two host cell regulators which inhibit propagation of complement pathways through distinct mechanisms. PMID: 22761385 Prostaglandin (PG)E2 has a novel role in CD46 functions, which might at least partly explain the diverse roles of PGE2 in T cell functions. PMID: 22544928 Three CD46 variants (rs7144, rs11118580, and rs2724384) were significantly associated with measles virus-specific IgG levels (P = 0.008, P = 0.026, and P = 0.018, respectively). PMID: 22357652 in systemic lupus erythematosus, the expression of leukocyte MCP at the mRNA level is closely related to disease activity PMID: 22247341 Data suggest that HBXIP upregulates CD46, CD55 and CD59 through p-ERK1/2/NF-kappaB signaling to protect breast cancer from complement-dependent cytotoxicity. PMID: 22293503 The strongest associations replicated in both cohorts were found for four SNPs within CD46 (p-value<10(-3)) and five SNPs within CFH (p-value<5x10(-3)). PMID: 22153652 Subjects previously immunized with measles-mumps-rubella vaccine were genotyped for 66 candidate single nucleotide polymorphisms in the CD46, SLAM and CD209 genes. PMID: 22086389 Mutations in the MCP gene seems to be a common etiology of atypical hemolytic uremic syndrome in Czech patients PMID: 21706448 protein expression is an unfavorable prognostic factor in breast cancer cases PMID: 21617523 Data sshow that variants in SFTPD, CD46 and IL1R1 are associated with IPD in both EA and AA. PMID: 21858107 the A304V mutation in the MCP gene is not strongly associated with RM or FL. PMID: 21840606 Show More Hide All
Involvement in disease	Hemolytic uremic syndrome atypical 2 (AHUS2)
Subcellular Location	Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane, Single-pass type I membrane protein
Tissue Specificity	Expressed by all cells except erythrocytes.
Database Links	HGNC: 6953 OMIM: 120920 KEGG: hsa:4179 STRING: 9606.ENSP00000313875 UniGene: Hs.510402

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Code	CSB-MP004939HU
Size	US$348Purchase it in Cusabio online store (only available for customers from the US)
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC₅₀ of human CD46 protein is 0.8333-1.054 ng/ml. Biological Activity Assay
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Recombinant Human Membrane cofactor protein(CD46) (Active)

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