Human membrane cofactor protein,MCP ELISA Kit

Instructions
Code CSB-E09216h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name CD46 molecule, complement regulatory protein
Alternative Names AHUS2, MCP, MGC26544, MIC10, TLX, TRA2.10, CD46 antigen, complement regulatory protein|antigen identified by monoclonal antibody TRA-2-10|complement membrane cofactor protein|measles virus receptor|
Abbreviation CD46
Uniprot No. P15529
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %93-102
1:2Average %90
Range %87-94
1:4Average %89
Range %85-93
1:8Average %92
Range %90-95
Recovery
The recovery of human MCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-99
EDTA plasma (n=4)9994-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.143 2.253 2.198 2.027
1001.544 1.563 1.554 1.383
501.077 1.170 1.124 0.953
250.774 0.796 0.785 0.614
12.50.513 0.526 0.520 0.349
6.250.368 0.391 0.380 0.209
3.120.273 0.293 0.283 0.112
00.170 0.172 0.171  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
Involvement in disease Hemolytic uremic syndrome atypical 2 (AHUS2)
Subcellular Location Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane, Single-pass type I membrane protein
Tissue Specificity Expressed by all cells except erythrocytes.
Database Links

HGNC: 6953

OMIM: 120920

KEGG: hsa:4179

STRING: 9606.ENSP00000313875

UniGene: Hs.510402

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.