Recombinant Human Muscle, skeletal receptor tyrosine-protein kinase(MUSK) is a partial-length active protein expressed in vitro E.coli expression system. This protein contains the extracellular domain (24-495aa) of human MUSK and carries an N-terminal 6xHis-tag. Its purity is greater than 90% as determined by SDS-PAGE. And its bio-activity was measured and confirmed through the ADP-Glo Kinase detection kit. The EC50 of MUSK is 2.65-3.58 μg/ml. The recombinant MUSK protein can be used for specific antibody production. Besides, it may find uses in the studies of MUSK-related signal transduction.
MUSK is a receptor tyrosine kinase (RTK) that exerts a critical role in the formation and maintenance of neuromuscular synapses through the agrin/LRP4-dependent MuSK signaling pathway. Mutations in genes including agrin, MuSK, Dok7, rapsyn, or AChR, fail to form proper neuromuscular junctions (NMJs) or maintain them, ultimately cause congenital myasthenia, resulting in muscle weakness and fatigue. Autoantibodies against the AChR, MUSK, or Lrp4 are responsible for Myasthenia gravis (MG).
||Greater than 90% as determined by SDS-PAGE.
||Measured by ADP-Glo Kinase detection kit. The EC50 of MUSK is 2.65-3.58 μg/ml.
||CMS9; FADS; MDK 4; MDK4; MGC126323; MGC126324; Muscle; Muscle associated receptor tyrosine kinase; Muscle skeletal receptor tyrosine kinase; Muscle skeletal receptor tyrosine protein kinase; Muscle specific kinase receptor; Muscle specific tyrosine kinase receptor; Muscle specific tyrosine protein kinase receptor; Muscle-specific kinase receptor; Muscle-specific tyrosine-protein kinase receptor; MuSK; MUSK_HUMAN; Neural fold somite kinase 1; Neural fold somite kinase 2; Neural fold somite kinase 3; Neural fold somite kinase1; Neural fold somite kinase2; Neural fold somite kinase3; Nsk 1; Nsk 2; Nsk 3; Nsk1; Nsk2; Nsk3; Receptor tyrosine kinase MuSK; Skeletal muscle receptor tyrosine kinase; skeletal receptor tyrosine-protein kinase
||Homo sapiens (Human)
||in vitro E.coli expression system
|Target Protein Sequence
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.