Human Muscle Skeletal Receptor Tyrosine Kinase (MUSK) ELISA Kit

Instructions
Code CSB-E15804h
Size 96T,5×96T,10×96T

Product Details

Target Name muscle, skeletal, receptor tyrosine kinase
Alternative Names RP11-104M22.1, MGC126323, MGC126324, skeletal muscle receptor tyrosine kinase
Abbreviation MUSK
Uniprot No. O15146
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MUSK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %97
Range %91-103
1:200Average %89
Range %84-95
1:400Average %95
Range %90-99
1:800Average %103
Range %99-109
Recovery
The recovery of human MUSK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-99
EDTA plasma (n=4)9894-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1002.675 2.552 2.614 2.510
502.226 2.376 2.301 2.197
251.672 1.594 1.633 1.529
12.50.792 0.816 0.804 0.700
6.250.446 0.427 0.437 0.333
3.120.236 0.231 0.234 0.130
1.560.180 0.189 0.185 0.081
00.106 0.102 0.104
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle
Involvement in disease Myasthenic syndrome, congenital, 9, associated with acetylcholine receptor deficiency (CMS9); Fetal akinesia deformation sequence (FADS)
Subcellular Location Cell junction, synapse, postsynaptic cell membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Tyr protein kinase family
Database Links

HGNC: 7525

OMIM: 208150

KEGG: hsa:4593

STRING: 9606.ENSP00000363571

UniGene: Hs.521653

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