Very nice to receive your inquiry.
Recombinant Human Muscle, skeletal receptor tyrosine-protein kinase(MUSK), partial
CSB-EP015241HU >> E.coli
Expression Region: 24-495aa; Partial, provide the complete extracellular domain of the Isoform 2.
Tag information:N-terminal GST-tagged.
Sequence:
LPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDSPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEEESEPEQDTKVFARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATISIAEWREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHLLSVPECSKLPSMHWDPTACARLPHLAFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSMTVIISIMSSFAIFVLLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDI
In addition, we also have successfully expressed this protein with our cell free expression system, which has already been validated the activity.
Recombinant Human Muscle, skeletal receptor tyrosine-protein kinase(MUSK),partial
CSB-CF015241HU
Expression Region: 24-495aa; Partial, provide the complete extracellular domain of the Isoform 2.
Tag information:N-terminal 6xHis-tagged
Sequence:
LPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDSPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEEESEPEQDTKVFARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATISIAEWREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHLLSVPECSKLPSMHWDPTACARLPHLAFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSMTVIISIMSSFAIFVLLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDI
The protein CSB-EP015241HU (1mg, Lot# 03633) you ordered was shipped with N-terminal GST-tag. Mw: 79.4kDa
According to your description, we guess you want the the protein with a much smaller tag to exclude the background interfernce, in order to observe the specific reactivity.
(1) If you only want the smaller tag, probably you can consider to order the protein expressed by another expression sytem, such as the protein expressed from Yeast expression system.
(2) If you still want the E.coli expressed protein but need a smaller tag, then we have to re-design and change another vector with smaller tag to do subclone, the price will be higher and lead time will be longer.
(So what's your requirement for the tag, as long as the tag is smaller than N-terminal GST-tagged ? Or do you have some specific tag?)
If you want to order the protein with N-terminal His-tag, the expected Mw of EP, CF, MP is 56.5kDa, and YP, BP protein is 54.5kDa.
