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| Code | CSB-MP822274HU |
| Size | $428 |
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| Description |
Recombinant human NECTIN4 protein is a mammalian cell-expressed partial length protein. Molecularly, this NECTIN4 protein is characterized by an N-terminal linker, internal human NECTIN4 DNA fragment (Gly32-Ser349), and a 10xHis tag at the C-terminus. Its purity is greater than 95% measured by SDS-PAGE. It has an apparent molecular weight of approximately 40 kDa on the gel. This slightly higher molecular mass results from glycosylation. This NECTIN4 protein contains low endotoxin, less than 1.0 EU/ug of the protein determined by the LAL method. Through a functional ELISA, this NECTIN4 protein has been validated to be active. It can bind to the anti-NECTIN4 antibody with an EC50 of 0.6029-0.7837 ng/mL. NECTIN4 participates in the intercellular connection and controls cell activities including cellular polarization, differentiation, motility, proliferation, and survival. Evidence has shown that NECTIN4 is overexpressed in multiple tumor cells but with a low expression in adult healthy tissues. NECTIN4, therefore, becomes a promising target for the diagnosis, prognosis, and treatment of many cancers. So far, a drug that combined the NECTIN4 antibody with MMAE has been developed and approved by FDA in December 2019 for the treatment of urothelial carcinoma. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized NECTIN4 at 2 μg/mL can bind anti-NECTIN4 antibody（CSB-RA822274A0HU）（enfortumab vedotin-like）, the EC50 is 0.6029-0.7837 ng/mL. |
| Target Names | NECTIN4 |
| Uniprot No. | Q96NY8 |
| Research Area | Cell Biology |
| Alternative Names | (Ig superfamily receptor LNIR)(Nectin cell adhesion molecule 4)(Poliovirus receptor-related protein 4) |
| Molecular Characterization |  |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 32-349aa |
| Target Protein Sequence | GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSAS |
| Mol. Weight | 36.9 kDa |
| Protein Length | Partial |
| Tag Info |
C-terminal 10xHis-tagged |
| Form |
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
There are currently no reviews for this product.
| Function |
Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.; (Microbial infection) Acts as a receptor for measles virus.
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| Gene References into Functions |
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| Involvement in disease | Ectodermal dysplasia-syndactyly syndrome 1 (EDSS1) |
| Subcellular Location | Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; [Processed poliovirus receptor-related protein 4]: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625). |
| Protein Families | Nectin family |
| Tissue Specificity | Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. |
| Database Links |
HGNC: 19688 OMIM: 609607 KEGG: hsa:81607 STRING: 9606.ENSP00000356991 UniGene: Hs.492490 |
