Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized NECTIN4 at 2 μg/mL can bind anti-NECTIN4 antibody（CSB-RA822274A0HU）（enfortumab vedotin-like）, the EC50 is 0.6029-0.7837 ng/mL.
Research Area
Cell Biology
Alternative Names
(Ig superfamily receptor LNIR)(Nectin cell adhesion molecule 4)(Poliovirus receptor-related protein 4)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
32-349aa
Target Protein Sequence
GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSAS
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant human NECTIN4 protein is a mammalian cell-expressed partial length protein. Molecularly, this NECTIN4 protein is characterized by an N-terminal linker, internal human NECTIN4 DNA fragment (Gly32-Ser349), and a 10xHis tag at the C-terminus. Its purity is greater than 95% measured by SDS-PAGE. It has an apparent molecular weight of approximately 40 kDa on the gel. This slightly higher molecular mass results from glycosylation. This NECTIN4 protein contains low endotoxin, less than 1.0 EU/ug of the protein determined by the LAL method. Through a functional ELISA, this NECTIN4 protein has been validated to be active. It can bind to the anti-NECTIN4 antibody with an EC50 of 0.6029-0.7837 ng/mL.
NECTIN4 participates in the intercellular connection and controls cell activities including cellular polarization, differentiation, motility, proliferation, and survival. Evidence has shown that NECTIN4 is overexpressed in multiple tumor cells but with a low expression in adult healthy tissues. NECTIN4, therefore, becomes a promising target for the diagnosis, prognosis, and treatment of many cancers. So far, a drug that combined the NECTIN4 antibody with MMAE has been developed and approved by FDA in December 2019 for the treatment of urothelial carcinoma.
