Name: Recombinant Human Nectin-4(NECTIN4),partial (Active)
Brand: CUSABIO
SKU: CSB-MP822274HU

Product Details

Description	Recombinant human NECTIN4 protein is a mammalian cell-expressed partial length protein. Molecularly, this NECTIN4 protein is characterized by an N-terminal linker, internal human NECTIN4 DNA fragment (Gly32-Ser349), and a 10xHis tag at the C-terminus. Its purity is greater than 95% measured by SDS-PAGE. It has an apparent molecular weight of approximately 40 kDa on the gel. This slightly higher molecular mass results from glycosylation. This NECTIN4 protein contains low endotoxin, less than 1.0 EU/ug of the protein determined by the LAL method. Through a functional ELISA, this NECTIN4 protein has been validated to be active. It can bind to the anti-NECTIN4 antibody with an EC₅₀ of 0.6029-0.7837 ng/mL. NECTIN4 participates in the intercellular connection and controls cell activities including cellular polarization, differentiation, motility, proliferation, and survival. Evidence has shown that NECTIN4 is overexpressed in multiple tumor cells but with a low expression in adult healthy tissues. NECTIN4, therefore, becomes a promising target for the diagnosis, prognosis, and treatment of many cancers. So far, a drug that combined the NECTIN4 antibody with MMAE has been developed and approved by FDA in December 2019 for the treatment of urothelial carcinoma.
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized NECTIN4 at 2 μg/mL can bind anti-NECTIN4 antibody（CSB-RA822274A0HU）（enfortumab vedotin-like）, the EC₅₀ is 0.6029-0.7837 ng/mL.
Target Names	NECTIN4
Uniprot No.	Q96NY8
Research Area	Cell Biology
Alternative Names	(Ig superfamily receptor LNIR)(Nectin cell adhesion molecule 4)(Poliovirus receptor-related protein 4)
Molecular Characterization
Species	Homo sapiens (Human)
Source	Mammalian cell
Expression Region	32-349aa
Target Protein Sequence	GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSAS
Mol. Weight	36.9 kDa
Protein Length	Partial
Tag Info	C-terminal 10xHis-tagged
Form	Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA	Please contact us to get it.

Target Background

Function (From Uniprot)	Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.; FUNCTION
Gene References into Functions	We suggest that Nectin-4 is a relevant prognostic factor and a therapeutic target in luminalB (HER2 negative) breast cancer. PMID: 28778498 Nectin-4 promotes cell-cell adhesion/aggregation, migration, and proliferation of ovarian tumor cells. PMID: 28038455 Nectin-4 is not only a BCSC marker but also a breast cancer metastasis marker PMID: 28600142 This study suggests that PVRL4 is post-transcriptionally regulated by miR-128 and miR-31. PMID: 27507538 High Nectin-4 expression is associated with neoplasms. PMID: 27013195 We show that Measles virus gains entry into MCF7, DLD-1, and HTB-20 cancer cells through a PVRL4-mediated macropinocytosis pathway and identified the typical cellular GTPase and kinase involved. PMID: 28250131 nectin-4 serves as a stimulatory co-receptor for the prolactin receptor by regulating the feedback inhibition of SOCS1 in the JAK2-STAT5a signaling pathway PMID: 28258213 Nectin-4 is both a new promising prognostic biomarker and specific therapeutic target for Triple-negative breast cancers PMID: 27998973 ADAM17 and ADAM10 cleave Nectin-4 and release soluble Nectin-4 (sN4). PMID: 28232483 The data presented in this study suggested that nectin-4 may be a therapeutic target for systemic lupus erythematous through affecting the cell apoptosis PMID: 26617807 Nectin-4 is critical for gallbladder cancer cell progression via PI3K/AKT pathway activation of Rac1. PMID: 26949052 Nectin-4 expression, when compared with control group, was higher in endometriotic lesions of patients having ovarian endometriosis and peritoneal endometriosis. This difference was significant in the endometrium of patients having endometriosis. PMID: 27328518 Transformation of breast cancer cells is dependent on PVRL4. PMID: 23682311 Nectin-4 is a significant prognostic predictor, and may play a critical role in pancreatic cancer. Nectin-4 may be novel therapeutic target for pancreatic cancer. PMID: 25888293 In airway epithelial cells, measles virus spread requires the nectin-4/afadin complex and is based on cytoplasm transfer between columnar cells. PMID: 25926640 The present study described clinical investigation of the EDSS1 identified in a large consanguineous family; DNA sequence analysis revealed a novel homozygous nonsense mutation (181C>T, p.Asp61*) in the PVRL4 gene. PMID: 25529316 Ovarian tissue expression and serum nectin 4 appear to be potential markers in ovarian cancer. PMID: 25019423 Authors show that the same key residues in the BC and FG loops of nectin-4 govern binding to the measles virus attachment protein hemagglutinin (H) and cell entry, nectin-4 homodimerization, and heterodimerization with nectin-1. PMID: 25275122 data outline a synergistic action of nectin-1 and -4 in the early steps of AJ formation and implicate this interaction in modulating the Rac1 signaling pathway PMID: 24577405 Ablation of nectin4 binding compromises CD46 usage by a hybrid vesicular stomatitis virus/measles virus. PMID: 24335299 Thus, while nectin-4 and CD46 interact functionally with the measles virus H protein beta4-beta5 hydrophobic groove, SLAM merely covers it. PMID: 23760251 Data suggest that expression of nectin-4 is up-regulated in eutopic endometrium of patients with endometriosis compared with control subjects. PMID: 22926846 We show that human nectin4 is fully functional as a canince distemper virus receptor. PMID: 23174504 structure of the membrane-distal domain of nectin-4 in complex with measles virus hemagglutinin (MV-H; structure shows nectin-4 binds the MV-H beta4-beta5 groove exclusively via its N-terminal IgV domain; the contact interface is dominated by hydrophobic interactions PMID: 23202587 crystals of nectin-4 diffracted to 1.8 A resolution and belonged to space group P2(1), with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 A, beta = 94.7 degrees PMID: 22869128 measles virus targets nectin-4 to emerge in the airways PMID: 22048310 Measles virus infection reduced PVRL4 surface expression in MCF7 cells, a property that is characteristic of receptor-associated viral infections. PMID: 21901103 The findings of study suggest that increased expression of Nectin-4 may indicate a worse prognosis in breast cancer patients. PMID: 21526486 Sequence analysis revealed a homozygous missense mutation (c.635C>G; p.Pro212Arg) in the recently reported PVRL4 gene causing EDSS1 PMID: 21346770 Ectoderma dysplasia-syndactyly syndrome. Second example of "nectinopathy". PMID: 21333831 nectin 4 may serve as a potential biomarker that helps discriminate benign gynecologic diseases from ovarian cancer in a panel with CA125. PMID: 20959669 Ectodermal dysplasia-syndactyly syndrome is the second known "nectinopathy" caused by mutations in a nectin adhesion molecule. PMID: 20691405 the active form of TACE is overexpressed in breast tumors and may indicate that TACE is responsible for Nectin-4 shedding not only in vitro but also in vivo PMID: 15784625 Nectin-4 was not detected in normal breast epithelium; by contrast Nectin-4 was expressed in ductal breast carcinoma. PMID: 17474988 Nectin-4 might play a significant role in lung carcinogenesis, and it should be a new candidate serum and tissue biomarker, as well as a therapeutic target. PMID: 19679554 Show More Hide All
Involvement in disease	Ectodermal dysplasia-syndactyly syndrome 1 (EDSS1)
Subcellular Location	Cell membrane, Single-pass type I membrane protein, Cell junction, adherens junction, Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254), SUBCELLULAR LOCATION: Processed poliovirus receptor-related protein 4: Secreted
Protein Families	Nectin family
Tissue Specificity	Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
Database Links	HGNC: 19688 OMIM: 609607 KEGG: hsa:81607 STRING: 9606.ENSP00000356991 UniGene: Hs.492490

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Code	CSB-MP822274HU
Size	$238
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized NECTIN4 at 2 μg/ml can bind anti-NECTIN4 antibody（CSB-RA822274A0HU）（enfortumab vedotin-like）, the EC₅₀ is 0.6029-0.7837 ng/mL. Biological Activity Assay
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Recombinant Human Nectin-4(NECTIN4),partial (Active)

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