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Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, Rat lung tissue, Rat brain tissue, Mouse lung tissue, Mouse brain tissue
All lanes: BCAP31 antibody at 4.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28, 35 kDa
Observed band size: 28 kDa
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IHC image of CSB-PA03425A0Rb diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA03425A0Rb diluted at 1:1200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA03425A0Rb at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating BCAP31 in Mouse liver tissue
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA03425A0Rb in Mouse liver tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA03425A0Rb (6µg) + Mouse liver tissue (500µg)
Lane 3: Mouse liver tissue (10µg)
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