BCAP31 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA834263A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate All lanes: BCAP31 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28, 35 kDa Observed band size: 28-36 kDa IHC image of CSB-RA834263A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA834263A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing PC3 cells stained with CSB-RA834263A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P51572

Target Names

BCAP31

Alternative Names

B-cell receptor-associated protein 31 (BCR-associated protein 31) (Bap31) (6C6-AG tumor-associated antigen) (Protein CDM) (p28), BCAP31, BAP31 DXS1357E

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human BCAP31

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To create the BCAP31 recombinant monoclonal antibody, several intricate steps are taken. The first involves obtaining and sequencing the BCAP31 monoclonal antibody gene. A vector carrying the antibody gene is then constructed and introduced into a host cell line for culture. During BCAP31 monoclonal antibody production, a synthesized peptide based on human BCAP31 serves as the immunogen. The BCAP31 recombinant monoclonal antibody is then purified using affinity chromatography and tested for specificity through ELISA, WB, IHC, IF, and FC applications. It is only reactive with human BCAP31 protein.

BCAP31 is a protein that is located on the endoplasmic reticulum (ER) membrane and is involved in a variety of cellular processes, including protein trafficking, calcium homeostasis, and apoptosis. It functions as a chaperone protein, helping to correctly fold and transport newly synthesized proteins to their appropriate destinations within the cell. BCAP31 has also been shown to be involved in the regulation of calcium levels in the ER and in the activation of the unfolded protein response (UPR) pathway. Additionally, mutations in the BCAP31 gene have been associated with a rare X-linked disorder called X-linked chondrodysplasia punctata type 2 (CDPX2), which is characterized by skeletal abnormalities and other developmental defects.

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Target Background

Function

Functions as a chaperone protein. Is one of the most abundant endoplasmic reticulum (ER) proteins. Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). Also serves as a cargo receptor for the export of transmembrane proteins. Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40. In response to ER stress, delocalizes from the ER-mitochondria contact sites and binds BCL2. May be involved in CASP8-mediated apoptosis.

Gene References into Functions

that BAP31 is a potential prognostic marker for colorectal cancer patients after surgery PMID: 29653744
BAP31 expression is an independent prognostic factor for overall survival of patients with postoperative hepatocellular carcinoma (HCC), and low expression levels of BAP31 in HCC may indicate poor outcomes of HCC patient after surgical resection. PMID: 25980696
results suggest that enhanced expression of csBAP31 contributes to poor survival of NSCLC cells PMID: 29145450
This patient's clinical features, muscle histopathology, brain MRI features, and family history were suggestive of mitochondrial encephalopathy. Whole exome sequencing research study confirmed the diagnosis of BCAP31-associated encephalopathy, DDCH syndrome. PMID: 28332767
Data characterized the biochemical properties of plant orthologs of human B-cell receptor-associated protein BAP31 (HsBAP31) termed 'plant BAP-like proteins' (PBL proteins). PMID: 27770627
Data show that tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced endoplasmic reticulum (ER) stress was triggered by caspase-8-mediated cleavage of B cell receptor-associated protein 31 (BAP31). PMID: 26212606
The C-terminal domain of BAP31 is exposed on the cell surface of human embryonic stem cells. PMID: 26102500
both BCAP31 and ABCD1 were associated with hepatic cholestasis and death before 1 year. Remarkably, a patient with an isolated deletion at the 3'-end of SLC6A8 had a similar severe phenotype as seen in BCAP31 deficiency PMID: 24597975
Transfected human respiratory syncytial virus SH protein co-localizes with transfected BAP31 in cells, and pulls down endogenous BAP31. PMID: 25854864
In the titel. PMID: 25044748
These findings provide, for the first time, mechanistic insights into how BAP31 regulates human embryonic stem cell stemness and survival via control of EpCAM expression. PMID: 24898727
Hypomethylation in BCAP31 is associated with breast cancer. PMID: 24395279
the lack of BAP31 disturbs endoplasmic reticulum (ER) metabolism and impacts the Golgi apparatus, highlighting an important role for BAP31 in ER-to-Golgi crosstalk. PMID: 24011989
BAP31 and BiP are essential for dislocation of SV40 from the endoplasmic reticulum to the cytosol. PMID: 21947079
Membrane-Associated RING-CH proteins MARCH VIII and MARCH IV associate with Bap31 and target CD81 and CD44 to lysosomes PMID: 21151997
Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction. PMID: 21183955
Contiguous deletion of the X-linked adrenoleukodystrophy gene (ABCD1) and DXS1357E: a novel neonatal phenotype similar to peroxisomal biogenesis disorders. PMID: 11992258
CASP8 cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol. PMID: 12668660
Data describe a novel human member of the protein tyrosine phosphatase-like B (PTPLB) family, an integral protein of the endoplasmic reticulum membrane, as a BAP31-interacting protein. PMID: 15024066
Cleavage of BAP31 leads to the generation of ER- localized, proapoptotic BAP20, which may mediate mitochondrion-ER cross talk through a Ca(2+)-dependent mechanism PMID: 15254227
BAP31 may play a role in regulating intracellular trafficking of CD11b/CD18 in neutrophils PMID: 15294914
We have characterized a molecular mechanism by which calnexin regulates ER-stress-mediated apoptosis in a manner independent of its chaperone functions but dependent of its binding to Bap31. PMID: 16858427
Bap31 performs quality control on class I molecules in two distinct phases: by exporting peptide-loaded class I molecules to the ER/Golgi, and by retrieving class I molecules that have lost peptides in the acidic post-ER environment. PMID: 17056546
Bap31 is a component of the endoplasmic reticulum (ER) quality control compartment and that it moves between the peripheral ER and a juxtanuclear ER or ER-related compartment distinct from the conventional ER-Golgi intermediate compartment. PMID: 18287538
BAP31 associates with the N terminus of one of its newly synthesized client proteins, the DeltaF508 mutant of CFTR, and promotes its retrotranslocation from the endoplasmic reticulum and degradation by the cytoplasmic 26S proteasome system. PMID: 18555783
Results identify B-cell-associated protein 31 as a novel binding partner of the high-risk human papillomavirus 16 E5 proteins and provide insight into how the E5 proteins may modulate the life cycle in differentiating cells. PMID: 18684816

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Involvement in disease

Deafness, dystonia, and cerebral hypomyelination (DDCH)

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein.

Protein Families

BCAP29/BCAP31 family

Tissue Specificity

Ubiquitous. Highly expressed in neurons and discrete endocrine cells.

Database Links

HGNC: 16695

OMIM: 300398

KEGG: hsa:10134

STRING: 9606.ENSP00000392330

UniGene: Hs.522817

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The Overview of Organelles Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Protein processing in endoplasmic reticulum

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