CDK12 Antibody

Datasheet

Code	CSB-PA998940
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA998940(CDK12 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA998940(CDK12 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
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Product Details
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Target Background

Product Details

Uniprot No.

Q9NYV4

Target Names

CDK12

Alternative Names

arginine/serine-rich antibody; Cdc2 related kinase arginine/serine rich antibody; CDC2 related protein kinase 7 antibody; Cdc2-related kinase antibody; CDC2-related protein kinase 7 antibody; Cdk12 antibody; CDK12_HUMAN antibody; Cell division cycle 2 related protein kinase 7 antibody; Cell division cycle 2-related protein kinase 7 antibody; Cell division protein kinase 12 antibody; CRK7 antibody; CRKR antibody; CrkRS antibody; Cyclin-dependent kinase 12 antibody; EC 2.7.11.22 antibody; hCDK12 antibody; kiaa0904 antibody; Pksc antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide of Human CDK12

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:2000
IHC	1:25-1:100

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.

Gene References into Functions

Cyclin K expression positively correlates with cell proliferation; knockdown of cyclin K or its cognate kinase CDK12 prevents the assembly of prereplicative complex in the G1 cell cycle phase. PMID: 29760377
CDK12 regulates prereplicative complex assembly to promote mammalian cell proliferation. PMID: 29760377
CDK12 gene amplification can contribute to the pathogenesis of breast cancer. PMID: 28334900
BRCA1 downregulation combined with CHK1 inhibition induced excessive amounts of DNA damage, resulting in an inability to complete the S-phase. Therefore, we suggest CHK1 inhibition as a strategy for targeting BRCA1- or CDK12-deficient tumors. PMID: 29025359
In Ovarian Cancer patients CDK12 loss is consistently associated with a particular genomic instability pattern characterized by hundreds of tandem duplications PMID: 26787835
Structures of CDK12/CycK complexes solved in the presence of AMP-PNP. PMID: 26597175
Data show that most mutations prevent formation of the cyclin-dependent kinase 12 (Cdk12)/cyclin K (CycK) complex, rendering the kinase inactive. PMID: 25712099
CDK12 affects RNA processing events in two distinct ways: Indirectly through generating factor-binding phospho-epitopes on the CTD of elongating RNAPII and directly through binding to specific factors PMID: 25429106
CDK12 and CDK13 losses in HCT116 cells preferentially affect expression of DNA damage response. PMID: 25561469
CDK12 plays an important role in cotranscriptional processing of c-FOS transcripts PMID: 25384976
CDK12 has properties that should confirm interest in its use as a biomarker. PMID: 24240700
many CDK12 mutations are an unrecognized cause of HR defects in ovarian cancers PMID: 24554720
CDK12 interacts with cyclin K and is required to maintain embryonnic stem cell self-renewal. PMID: 22547058
Cyclin K1 is the primary cyclin partner for CDK12/CrkRS and it is required for activation of CDK12/CrkRS to phosphorylate the C-terminal domain of RNA Pol II. PMID: 22988298
through regulation of expression of DNA damage response genes, CycK/Cdk12 protects cells from genomic instability PMID: 22012619
Data show that siRNA knockdown of hCDK12 in HeLa cells results in alterations in the CTD phosphorylation state. PMID: 20952539
CRK7 modifies MAP kinases regulation and resistance to estrogen signaling inhibitors in breast cancers. PMID: 19651820

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Involvement in disease

Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.

Subcellular Location

Nucleus. Nucleus speckle. Note=Colocalized with nuclear speckles throughout interphase.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 24224

OMIM: 615514

KEGG: hsa:51755

STRING: 9606.ENSP00000398880

UniGene: Hs.345028

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Protein Service

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Related pathways

Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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