Thanks for your inquiry. Proteins will be supplied soluble in solution.
It is not certain for the protein to be expressed in the supernatant or precipitation, but our proteins are provided with folding correctly.
If you need it supplied in an amine free buffer (so not Tris). We can do this. Please mention this requirement in your official order. We can try to provide PBS buffer, this is not charged.
Recombinant Human Cyclin-dependent kinase 12(CDK12),partial
CSB-YP882147HU >> Yeast
CSB-EP882147HU >> E.coli
CSB-BP882147HU >> Baculovirus
CSB-MP882147HU >> Mammalian cell
Expression Region:727-1020aa;Partial,Protein kinase domain.
Tag information:EP,YP,BP,MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP:N-terminal 6xHis-tagged;EP,BP,MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:
FDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFL