Name: CDK12 Antibody
Brand: CUSABIO
SKU: CSB-PA882147LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CDK12 Polyclonal antibody

Uniprot No.

Q9NYV4

Target Names

CDK12

Alternative Names

arginine/serine-rich antibody; Cdc2 related kinase arginine/serine rich antibody; CDC2 related protein kinase 7 antibody; Cdc2-related kinase antibody; CDC2-related protein kinase 7 antibody; Cdk12 antibody; CDK12_HUMAN antibody; Cell division cycle 2 related protein kinase 7 antibody; Cell division cycle 2-related protein kinase 7 antibody; Cell division protein kinase 12 antibody; CRK7 antibody; CRKR antibody; CrkRS antibody; Cyclin-dependent kinase 12 antibody; EC 2.7.11.22 antibody; hCDK12 antibody; kiaa0904 antibody; Pksc antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Cyclin-dependent kinase 12 protein (1309-1490AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CDK12 Antibody (Product code: CSB-PA882147LA01HU) is Non-conjugated. For CDK12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA882147LB01HU	CDK12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA882147LC01HU	CDK12 Antibody, FITC conjugated
Biotin	CSB-PA882147LD01HU	CDK12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The CDK12 antibody is a polyclonal IgG antibody developed in a rabbit. The immunogen is the recombinant human CDK12 protein (1309-1490aa). The purity of this CDK12 antibody exceeds 95% using protein G purification. This CDK12 antibody is specifically reactive with human species. The reactivity of this OCLN antibody with the CDK12 protein has been confirmed by ELISA and WB assays.

CDK12 is a kinase enzyme that regulates the expression of genes involved in various cellular processes, including transcription, DNA damage response and repair, and cell cycle regulation. Mutations in the CDK12 gene have been identified in a subset of tumors, including ovarian, breast, and prostate cancers.

Target Background

Function

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.

Gene References into Functions

Cyclin K expression positively correlates with cell proliferation; knockdown of cyclin K or its cognate kinase CDK12 prevents the assembly of prereplicative complex in the G1 cell cycle phase. PMID: 29760377
CDK12 regulates prereplicative complex assembly to promote mammalian cell proliferation. PMID: 29760377
CDK12 gene amplification can contribute to the pathogenesis of breast cancer. PMID: 28334900
BRCA1 downregulation combined with CHK1 inhibition induced excessive amounts of DNA damage, resulting in an inability to complete the S-phase. Therefore, we suggest CHK1 inhibition as a strategy for targeting BRCA1- or CDK12-deficient tumors. PMID: 29025359
In Ovarian Cancer patients CDK12 loss is consistently associated with a particular genomic instability pattern characterized by hundreds of tandem duplications PMID: 26787835
Structures of CDK12/CycK complexes solved in the presence of AMP-PNP. PMID: 26597175
Data show that most mutations prevent formation of the cyclin-dependent kinase 12 (Cdk12)/cyclin K (CycK) complex, rendering the kinase inactive. PMID: 25712099
CDK12 affects RNA processing events in two distinct ways: Indirectly through generating factor-binding phospho-epitopes on the CTD of elongating RNAPII and directly through binding to specific factors PMID: 25429106
CDK12 and CDK13 losses in HCT116 cells preferentially affect expression of DNA damage response. PMID: 25561469
CDK12 plays an important role in cotranscriptional processing of c-FOS transcripts PMID: 25384976
CDK12 has properties that should confirm interest in its use as a biomarker. PMID: 24240700
many CDK12 mutations are an unrecognized cause of HR defects in ovarian cancers PMID: 24554720
CDK12 interacts with cyclin K and is required to maintain embryonnic stem cell self-renewal. PMID: 22547058
Cyclin K1 is the primary cyclin partner for CDK12/CrkRS and it is required for activation of CDK12/CrkRS to phosphorylate the C-terminal domain of RNA Pol II. PMID: 22988298
through regulation of expression of DNA damage response genes, CycK/Cdk12 protects cells from genomic instability PMID: 22012619
Data show that siRNA knockdown of hCDK12 in HeLa cells results in alterations in the CTD phosphorylation state. PMID: 20952539
CRK7 modifies MAP kinases regulation and resistance to estrogen signaling inhibitors in breast cancers. PMID: 19651820

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Involvement in disease

Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.

Subcellular Location

Nucleus. Nucleus speckle. Note=Colocalized with nuclear speckles throughout interphase.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 24224

OMIM: 615514

KEGG: hsa:51755

STRING: 9606.ENSP00000398880

UniGene: Hs.345028

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Code	CSB-PA882147LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: CDK12 antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 165, 164, 142 kDa Observed band size: 165 kDa
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CDK12 Antibody

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