EPHB3 Antibody

Datasheet

Code	CSB-PA007731LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: EPHB3 antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 111 kDa Observed band size: 111 kDa Immunofluorescent analysis of Hela cells using CSB-PA007731LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EPHB3 Polyclonal antibody

Uniprot No.

P54753

Target Names

EPHB3

Alternative Names

Cek10 antibody; EK2 antibody; Embryonic kinase 2 antibody; EPH Like Tyrosine Kinase 2 antibody; EPH receptor B3 antibody; EPH-like kinase 2 antibody; ephb3 antibody; EPHB3_HUMAN antibody; Ephrin receptor EphB3 antibody; Ephrin type B receptor 3 antibody; Ephrin type-B receptor 3 antibody; ETK2 antibody; hEK2 antibody; Human Embryo Kinase 2 antibody; Mdk5 antibody; Sek4 antibody; TYRO6 antibody; Tyrosine protein kinase receptor HEK2 antibody; Tyrosine protein kinase TYRO6 antibody; Tyrosine-protein kinase TYRO6 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Ephrin type-B receptor 3 protein (261-390AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EPHB3 Antibody (Product code: CSB-PA007731LA01HU) is Non-conjugated. For EPHB3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA007731LB01HU	EPHB3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA007731LC01HU	EPHB3 Antibody, FITC conjugated
Biotin	CSB-PA007731LD01HU	EPHB3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Gene References into Functions

These results indicated the suppressive effect of Form on colon carcinoma cell proliferation and invasion, possibly via miR149induced EphB3 downregulation and the inhibition of the PI3K/AKT and STAT3 signaling pathways. PMID: 29620230
work suggested that EphB3 acted as a tumor promoter in Papillary Thyroid Cancer by increasing the in vitro migration as well as the in vivo metastasis of Papillary Thyroid Cancer cells through regulating the activities of Vav2 and Rho GTPases in a kinase-dependent manner. PMID: 27986811
These results show that EphB3 protein is lost in ovarian serous carcinoma and is associated with tumor grade and FIGO stage, which indicate that EphB3 protein may play a role in carcinogenesis of ovarian serous carcinoma and may be used as a molecular marker for prognosis. PMID: 28120491
results identify EPHB3 as a novel target of SNAIL1 and suggest that disabling EPHB3 signaling is an important aspect to eliminate a roadblock at the onset of EMT processes. PMID: 25277775
These results uncover enhancer decommissioning as a mechanism for transcriptional silencing of the EPHB3 tumor suppressor. PMID: 24707046
EphB3 suppresses non-small-cell lung cancer metastasis via a PP2A/RACK1/Akt signalling complex PMID: 22314363
Our work shows that EphB3 is consistently expressed by malignant T lymphocytes, most frequently in combination with EphB6, and that stimulation with their common ligands strongly suppresses Fas-induced apoptosis in these cells. PMID: 22039307
EphB3 provides critical support to the development and progression of NSCLC by stimulating cell growth, migration, and survival. PMID: 21266352
Ephrin B3 receptor regulates the synthesis and release of D-serine in astrocytes, which may have important implications on synaptic transmission and plasticity. PMID: 21106840
while catalytic activity of EphB3 is required for inhibition of integrin-mediated cell adhesion, a distinct signaling pathway to Rho GTPases shared by WT- and KD-EphB3 receptor mediates inhibition of directional cell migration PMID: 15536074
EphB3-ephrin-B interaction promotes mesenchymal-to-epithelial transition (MET) by re-establishing epithelial cell-cell junctions and such an MET-promoting effect contributes to EphB3-mediated tumor suppression. PMID: 19483190
EPHB3, MASP1 and SST map to 3q26.2-q29 and may have roles in squamous cell carcinoma of the lung PMID: 19607727

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell projection, dendrite.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 3394

OMIM: 601839

KEGG: hsa:2049

STRING: 9606.ENSP00000332118

UniGene: Hs.2913

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Protein Service

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The Overview of One Category of Important Cell Receptors: Receptor Tyrosine Kinases Protein Phosphorylation: The Dynamic Language of Cellular Regulation Protein Kinases Overview: Definition, Types, Function, Activation Mechanism, etc. EphA3-A Regulator of Cell Adhesion and Migration Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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