Recombinant Human Cyclic AMP-dependent transcription factor ATF-6 alpha(ATF6),partial

Code CSB-YP002277HU
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Source Yeast
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Code CSB-EP002277HU
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Source E.coli
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Code CSB-EP002277HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002277HU
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Source Baculovirus
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Code CSB-MP002277HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ATF6
Uniprot No. P18850
Alternative Names Activating transcription factor 6 alpha; Activating transcription factor 6; ATF 6; ATF6 alpha; ATF6; ATF6-alpha; ATF6A; ATF6A_HUMAN; cAMP dependent transcription factor ATF 6 alpha; cAMP-dependent transcription factor ATF-6 alpha; Cyclic AMP dependent transcription factor ATF 6 alpha; DKFZp686P2194; ESTM49; FLJ21663; Processed cyclic AMP dependent transcription factor ATF 6 alpha; Processed cyclic AMP-dependent transcription factor ATF-6 alpha
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
Gene References into Functions
  1. 147 is a pro-drug that preferentially activates ATF6 signaling through a mechanism involving localized metabolic activation and selective covalent modification of endoplasmic reticulumresident proteins that regulate ATF6 activity. PMID: 30084354
  2. sustained intestinal activation of ATF6 in the colon to promote dysbiosis and microbiota-dependent tumorigenesis. PMID: 30063920
  3. results support a critical role of ATF6alpha in the establishment and maintenance of cellular senescence in normal human fibroblasts via the up-regulation of a COX2/PGE2 intracrine pathway. PMID: 28803844
  4. The results demonstrated that high expression of activated ATF6 aggravates ER stressinduced VEC apoptosis through the mitochondrial apoptotic pathway. Furthermore, in response to ER stress, ATF6 upregulates the expression of caspase3, caspase9, CHOP, cytochrome c and Bax/Bcl2. PMID: 29512699
  5. Reporters constructed to monitor each mechanism show that phenobarbital-induced endoplasmic reticulum membrane expansion depends on transmembrane domain-induced ATF6 PMID: 30086303
  6. ATF6 plays a distinct role in viral protein stability and the host uses different cleavage strategies, rather than conventional cleavage by generating p50ATF6, to combat viral infection. PMID: 29386036
  7. Three branches of the Unfolded Protein Response (UPR) have been described, including the activation of the inositol-requiring enzyme 1 (IRE1), the pancreatic ER kinase (PKR)-like ER kinase (PERK), and the activating transcription factor 6 (ATF6). PMID: 28105371
  8. The expression of ASNS was significantly elevated when ATF6 was over expressed. The expressions of these 2 genes were both decreased in hepatocellular carcinoma (HCC)patients, and it was more significantly with ASNS. The mRNA levels of ASNS and ATF6 were positively correlated with each other. rs34050735 was associated with HCC in the case-control study and also an independent predictor of overall survival of HCC patients. PMID: 28629319
  9. Human ATF6 mutations interrupt distinct sequential steps of the ATF6 activation mechanism. PMID: 28028229
  10. Findings indicate a central role for activating transcription factor 6 (ATF6alpha) in the establishment of morphological features of senescence in normal primary fibroblasts. PMID: 27563820
  11. A novel homozygous c.1691A>G (p.(Asp564Gly)) ATF6 mutation was identified in two siblings with autosomal recessive cone-rod dystrophy. PMID: 28812650
  12. This review shown that the achromatopsia arising from genetic mutations in Activating Transcription Factor 6 (ATF6). PMID: 27117871
  13. In summary, our study revealed a negative regulation of the UPR transducer ATF6 through post-translational SUMOylation. The information from this study will not only increase our understanding of the fine-tuning regulation of the UPR signaling but will also be informative to the modulation of the UPR for therapeutic benefits. PMID: 29061306
  14. Low ATF6 expression is associated with cancer. PMID: 28157699
  15. Activating transcription factor 6 (ATF6alpha) pathway and ER-associated protein degradation (ERAD) are elevated in salivary glands of Sjogren's syndrome patients (SS). PMID: 27461470
  16. A transcription factor complex consisting of ATF6 (an endoplasmic reticulum-resident factor) and C/EBP-beta is required for the IFN-gamma-induced expression of DAPK1 IFN-gamma-induced proteolytic processing of ATF6 and phosphorylation of C/EBP-beta are obligatory for the formation of this transcriptional complex PMID: 27590344
  17. Authors observed the cleavage of ATF6, the phosphorylation of MRLC, and the expression of death-associated protein kinase (DAPK1) by western blotting; the transcription of DAPK1 by RT-PCR; and the subcellular localization of ATF6 and mAtg9 by immunofluorescence. PMID: 27085326
  18. Genetic Variations in ATF6 (rs2070150) is not Associated with Hepatocellular Carcinoma in Thai Patients with Hepatitis B Virus Infection. PMID: 26925648
  19. We found that HCS identified compounds which inhibited ATF6 nuclear translocation with high specificity, as confirmed by the luciferase reporter assay and western blot analysis PMID: 26707144
  20. results identify a role for DREAM silencing in the activation of ATF6 signaling, which promotes early neuroprotection in HD. PMID: 26752648
  21. Endoplasmic reticulum stress related factor ATF6 and caspase-12 trigger apoptosis in neonatal hypoxic-ischemic encephalopathy. PMID: 26261584
  22. These results confirm that HIV infection activates stress-response components and that antiretroviral therapy contributes to changes in the unfolded protein response activation profile. PMID: 25976933
  23. Defective podocyte insulin signaling through p85-XBP1 promotes ATF6-dependent maladaptive ER-stress response in diabetic nephropathy. PMID: 25754093
  24. protein expression were significantly higher in the placentas of women with early and late SPE than in the control women, whereas there were no differences in ATF6 and Ire1 mRNA and protein. PMID: 25675914
  25. autosomal recessive achromatopsia is caused by a frameshift mutation in ATF6 in this Pakistani family PMID: 26063662
  26. A crucial and unexpected role for ATF6A in human foveal development and cone function. PMID: 26029869
  27. ATF6 preferentially reduces the secretion and extracellular aggregation of destabilized, aggregation-prone variants of the amyloidogenic protein TTR, as compared to stable WT TTR and nonamyloidogenic TTR variants. PMID: 25444553
  28. The genetic variation in ATF6 is associated with pre-diabetes and has interactive effects with BMI on pre-diabetes in the Chinese Han population. PMID: 25302688
  29. Data show that silver nanoparticles induce activating transcription factor-6 (ATF-6) degradation, leading to activation of the NLRP-3 inflammasome and pyroptosis. PMID: 25593314
  30. our data demonstrate that CiC expression is activated during ER stress through the binding of ATF6alpha and XBP1 to an UPRE element located in the proximal promoter of Cic gene. PMID: 25450523
  31. Data shows that mutation of putative p38 MAPK phosphorylation sites in ATF6 suppresses its transcriptional induction of DAPK1. PMID: 25135476
  32. activating transcription factor 6 single polymorphism has a role in increased body mass index after kidney transplantation PMID: 22099811
  33. Activation of the ATF6 pathways of the UPR is sustained along with PERK signaling in melanoma cells subjected to pharmacological ER stress, and that this plays an important role in protecting melanoma cells from ER stressinduced apoptosis. PMID: 24240056
  34. ATF6 binds to the promoter of XBP1 and enhances the XBP1S expression in OA cartilage. PMID: 24269637
  35. activation of the ATF6 pathway of the UPR limits ATZ-dependent cell toxicity by selectively promoting ER-associated degradation of ATZ PMID: 21976666
  36. These studies indicate that activation of either IRE1, ATF6, or PERK prevents mutant rhodopsin from accumulating in the cells. PMID: 24664756
  37. Tick-borne encephalitis virus infection activates the IRE1 pathway and triggers expression of cleaved transcription factor 6 (ATF6) which suggest activation of ATF6 pathway. PMID: 24177270
  38. This study showed that the two compounds, polyP and isoquercitrin, have a co-enhancing effect on bone mineral formation and in turn might be of potential therapeutic value for prevention/treatment of osteoporosis. PMID: 24726443
  39. ATF6 mRNA expression was significantly decreased as the disease progressed PMID: 24302549
  40. these results indicate that ATF6alpha can regulate de novo cholesterol synthesis through stimulation of cholesterogenic gene expression. PMID: 23924739
  41. Mediator subunit MED25 plays a critical role in this process and identify a MED25 domain that serves as a docking site on Mediator for the ATF6alpha transcription activation domain. PMID: 23864652
  42. ATF6 represents a novel type of ERAD-Lm substrate requiring SEL1L for degradation despite its transmembrane nature. PMID: 24043630
  43. results demonstrate that ER stress-related proteins, particularly ATF6 and its downstream molecule CHOP, are involved in ox-LDL-induced cholesterol accumulation and apoptosis in macrophages PMID: 23037953
  44. ATF6 activation is significantly higher for the CASPR2-D1129H compared with the wild-type protein. PMID: 22872700
  45. one of three endoplasmic reticulum transmembrane protein sensors that signals the unfolded protein response [review] PMID: 22917505
  46. Data show that transfection of ORMDL3 in bronchial epithelial cells induced expression of MMP-9, ADAM-8, CCL-20, IL-8, CXCL-10, CXCL-11, oligoadenylate synthetases (OAS) genes, and selectively activated activating transcription factor 6 (ATF6). PMID: 23011799
  47. This study indicates that selectively activating ATF6 or PERK prevents mutant rhodopsin from accumulating in retinal cells. PMID: 22956602
  48. ATF6alpha functions at least in part by recruiting to the endoplasmic reticulum stress response enhancer elements of ER stress response genes a collection of RNA polymerase II coregulatory complexes. PMID: 22577136
  49. Increased expression of Endoplasmic Reticulum (ER) stress markers, GRP78, ATF6 and CHOP, in endometrioid endometrial carcinomas suggests a role for ER stress in endometrial cancer. PMID: 22146569
  50. BMP2 induces osteoblast differentiation through Runx2-dependent ATF6 expression, which directly regulates Oc transcription. PMID: 22102412

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Involvement in disease Achromatopsia 7 (ACHM7)
Subcellular Location Endoplasmic reticulum membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Nucleus
Protein Families BZIP family, ATF subfamily
Tissue Specificity Ubiquitous.
Database Links

HGNC: 791

OMIM: 605537

KEGG: hsa:22926

STRING: 9606.ENSP00000356919

UniGene: Hs.492740

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