Recombinant Human DNA replication licensing factor MCM3(MCM3) ,partial

Code CSB-YP013591HU
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Source Yeast
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Code CSB-EP013591HU
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Source E.coli
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Code CSB-EP013591HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013591HU
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Source Baculovirus
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Code CSB-MP013591HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names MCM3
Uniprot No. P25205
Alternative Names Cervical cancer proto oncogene 5; DNA polymerase alpha holoenzyme associated P1; DNA polymerase alpha holoenzyme associated protein P1; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor mcm3; HCC 5; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MCM 3; mcm3; MCM3 minichromosome maintenance deficient 3; MCM3_HUMAN; MGC1157; Minichromosome maintenance complex component 3; Minichromosome maintenance deficient 3; Minichromosome maintenance protein 3; P1 h; P1 MCM3; P1 Protein; P1-MCM3; P1.h; p102; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta; RLFB
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Gene References into Functions
  1. this study shows that MCM3 is a novel proliferation marker in oral squamous cell carcinoma PMID: 27258565
  2. High MCM3 expression is associated with bone metastasis and advanced human prostate cancer. PMID: 28424404
  3. reveal the Minichromosome Maintenance Complex to be a critical and directly regulated node within the miR-183 signaling network in MYCN-amplified neuroblastoma cells. Randomly selected MCMs 3 and 5 were experimentally confirmed as direct targets of miR-183. PMID: 27239679
  4. The results of our preliminary study emphasize the need for future research on MCM-3 as a sensitive proliferation marker, providing an alternative to Ki-67, in cases of various major salivary gland epithelial tumors in children and adolescents. PMID: 28547962
  5. These data establish new functions for KEAP1 within the nucleus and identify MCM3 as a novel substrate of the KEAP1-CUL3-RBX1 E3 ligase. PMID: 27621311
  6. The aim of this study is to analyze the immunoexpression of Ki67, p53, MCM3 and PCNA markers in epithelial remnants of dental follicles of impacted teeth and to identify a possible correlation between the immunoexpression of these markers in dentigerous cysts and keratocystic odontogenic tumors. PMID: 27516012
  7. High MCM-3 expression correlated with Cutaneous T-cell Lymphomas. PMID: 26504025
  8. normal DNA replication and replication checkpoint activation is regulated through the novel phosphorylation of MCM3 by Chk1 PMID: 25809478
  9. The relatively lower MCM3 protein expression in FVPTC. PMID: 23821456
  10. Expression of KB cell MCM3 was not affected by doxorubicin. PMID: 24344040
  11. present study aimed to investigate the relationship between expression of minichromosome maintenance proteins (MCM-3, MCM-7), metallothioneins (MT-I/II, MT-III), and Ki-67 in 103 ovarian cancer cases, mostly of the serous histological type PMID: 24324072
  12. High MCM3 expression is associated with mucoepidermoid carcinomas and adenoid cystic carcinomas in salivary gland tumors. PMID: 23886132
  13. Relatively lower MCM3 protein expression in follicular variant of papillary thyroid carcinoma comparing to classical type could be due to a different tumorigenic pathway favored in this type of tissue. PMID: 23821456
  14. These findings provide validation of the utility of MCM3 expression as an independent biomarker for prognostication of patients with primary melanoma PMID: 22805320
  15. excess of MCM3 up-regulates the phosphorylation of CHK1 Ser-345 and CDK2 Thr-14. PMID: 21965652
  16. MCM3 gene expression is decreased in follicular variant of papillary thyroid carcinoma, contrary to expectation. PMID: 21509594
  17. Using U373MG cells we found that HCMV IE86 protein was bound to Mcm3, but did not inhibit the cellular DNA synthesis. PMID: 20545442
  18. MCM3 may be a more reliable proliferation marker than Ki-67 in accessing the growth of tumor and evaluating tumor aggressiveness of papillary thyroid carcinoma. PMID: 19818763
  19. The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with this protein PMID: 12226073
  20. Elevated MCM3 protein expression is associated with neoplasms PMID: 15623617
  21. MCM3 is overexpressed in human astrocytic tumors and elicits a cancer-restricted humoral immune response in some patients with brain neoplasms PMID: 15671553
  22. ORC1 and Mcm3 proteins are required for DNA replication in protein extracts from human cells PMID: 16537544
  23. analysis of carboxyl-terminal MCM3 phosphorylation sites and their motifs PMID: 17244605
  24. These data reveal that CDK-dependent MCM3 phosphorylation contributes to the regulated formation of the MCM2-7 complex. PMID: 18524952

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Subcellular Location Nucleus
Protein Families MCM family
Database Links

HGNC: 6945

OMIM: 602693

KEGG: hsa:4172

STRING: 9606.ENSP00000229854

UniGene: Hs.179565

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