MCM3 Antibody

Datasheet
Code CSB-PA110553
Former Code(s) CSB-PA212238
Size US$299
Uniprot No. P25205
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA110553(MCM3 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: K562 cells, Jurkat cells, hela cells, 293T cells, Primary antibody: CSB-PA110553(MCM3 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Immunogen Fusion protein of Human MCM3
Raised in Rabbit
Species Reactivity Human,Mouse
Tested Applications ELISA,WB,IHC;ELISA:1:1000-1:5000,WB:1:500-1:2000,IHC:1:25-1:100
Relevance The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.
Form Liquid
Conjugate Non-conjugated
Storage Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method Antigen affinity purification
Isotype IgG
Alias minichromosome maintenance complex component 3
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names MCM3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Subcellular Location Nucleus
Protein Families MCM family
Database Links

HGNC: 6945

OMIM: 602693

KEGG: hsa:4172

STRING: 9606.ENSP00000229854

UniGene: Hs.179565

Pathway Cell cycle

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