Recombinant Human Forkhead box protein O3(FOXO3),partial

Code CSB-YP008836HU1
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Source Yeast
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Code CSB-EP008836HU1-B
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP008836HU1
Size Pls inquire
Source Baculovirus
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Product Details

Purity >85% (SDS-PAGE)
Target Names FOXO3
Uniprot No. O43524
Alternative Names AF6q21; AF6q21 protein; DKFZp781A0677; FKHR2; FKHRL 1; FKHRL1; FKHRL1P2; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1; Forkhead box O3; Forkhead box O3A; Forkhead box protein O3; Forkhead box protein O3A; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1; Forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma like 1; Forkhead in rhabdomyosarcoma-like 1; FOX O3A; FOXO2; foxo3; FOXO3_HUMAN; FOXO3A; MGC12739; MGC31925
Species Homo sapiens (Human)
Protein Length partial
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

(From Uniprot)
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC
Gene References into Functions
  1. role in autophagy activation and the maintenance of intracellular homeostasis in inflamed odontoblasts PMID: 29551204
  2. Low FOXO3A expression is associated with colorectal cancer. PMID: 30066886
  3. FoxO3a was overexpressed in 64.71% cases of hepatocellular carcinoma (HCC). FoxO3a overexpression was associated with aggressive phenotypes of HCC, such as histologic grade, stage, and small vessel invasion. FoxO3a overexpression was also correlated with poor disease-free survival. Downregulation of FoxO3a in a HepG2 cell line inhibited cell proliferation and migration. PMID: 29365018
  4. stable knockdown of FOXO3, NCOA3, and TCF7L2 restored growth in low glucose but reduced MEK/MAPK phosphorylation, reduced anchorage-independent growth, and modulated expressions of GLUT1 and Ras pathway related proteins. PMID: 29301589
  5. the inhibition of miR-9 could induce apoptosis in cervical cancer by targeting FOXO3. PMID: 29602130
  6. Study in three European populations present experimental evidence for a functional link between common intronic variants in FOXO3 and human longevity. PMID: 29234056
  7. circRNA-FOXO3 expression was decreased in NSCLC cells and tissue samples. It can inhibit the development of NSCLC cells as a ceRNA through sponging miR-155 and releasing FOXO3 level. PMID: 29620202
  8. The protein expression levels of several autophagy makers, such as LC3I, LC3II and Beclin-1, were higher in FOXO3 plasmid-transfected AGS cells cultured in an acidic microenvironment than in control cells, while P62 protein expression levels were clearly decreased in FOXO3 plasmid-transfected cells compared with control cells. PMID: 30138933
  9. Study suggests that miR-487a-3p might repress CTLA4 and FOXO3 by binding to their 3'UTRs and contribute to the development of T1D. PMID: 29859273
  10. Findings determined that the crucial regions corresponding to the SP1 binding sites located between 2,000 and 1,037 bp were essential for FoxO3a transcriptional activity. Furthermore, FoxO3a transcription was upregulated in response to hypoxic and oxidative stress in colorectal tumor cells (CRC), indicating that the interaction between SP1 and FoxO3a may have important implications in CRC progression. PMID: 29565456
  11. FOXO3a expression correlated with adverse clinicopathological features, such as lymph node metastasis, perineural invasion and higher Ki-67 proliferation index in triple-negative breast cancers. PMID: 29588373
  12. human FOXO3B locus encodes a bona fide human gene; unlike FOXO3A, FOXO3B is cytosolically localized in both the presence and absence of active Akt PMID: 29925039
  13. FoxO3a overexpression increased the transcription and protein expression of Bcl2like protein 11 and cyclindependent kinase inhibitor 1B, and inhibited cyclin D1 transcription and expression. PMID: 29257235
  14. miR-132 negatively regulates palmitate induced NLRP3 inflammasome activation through FOXO3 down-regulation in THP-1 cells. PMID: 29258239
  15. these data ascertain the existence of an H2O2-sensitive PRDX1-FOXO3 signaling axis that fine tunes FOXO3 activity toward the transcription of gene targets in response to oxidative stress. PMID: 28398822
  16. results suggested that SIRT1 deficiency in Bladder cancer cells could suppress cell viability by activating antioxidant response and inducing cell cycle arrest possibly via FOXO3a-related pathways. PMID: 29147649
  17. these results suggest that miR-30b plays important roles in kynurenine-induced increase of FOXO3 expression PMID: 28905195
  18. Authors found that miR-629 negatively regulated FOXO3 protein expression and decreased the activity of a luciferase reporter construct containing the FOXO3 3'-untranslated region. These results show that miR-629 regulates FOXO3 at the posttranscriptional level, resulting in enhanced cell proliferation and invasion of pancreatic carcinoma. PMID: 29072689
  19. auranofin could regulate the Her2/Akt/FOXO3 signaling pathway in SKOV3 cells and be used as a potential antitumor agent considering the expression of MUC4 in ovarian cancer patients. PMID: 28765909
  20. FNDC5 gene interactions with candidate genes FOXOA3 and APOE PMID: 29143599
  21. Results indicate a mechanism for beta-arrestin1 in the regulation of the prostate cancer procession through inhibiting FOXO3a. PMID: 29676828
  22. The findings of this study indicated that FoxO3a knockdown conferred neuroprotective effects after TBI through inhibiting the activation of neuronal autophagy. PMID: 28889023
  23. Low FOXO3A expression is associated with cancer. PMID: 29533771
  24. Data show that forkhead box O3 protein (FOXO3) silencing could inhibit mitophagy and mitochondrial dysfunction induced by manganese chloride (MnCl2). PMID: 28661534
  25. Report an inverse relationship of age with human serum FOXO3A and SIRT3 levels. PMID: 28526626
  26. FOXO3 longevity interactome on chromosome 6 has been described. PMID: 28722347
  27. miR-223-3p regulated cell chemo-sensitivity by targeting FOXO3 in prostatic cancer (PCa) both in vitro and in vivo, providing new potential therapeutic strategy for PCa treatment. PMID: 29518547
  28. H cordata promotes the activation of HIF-1A-FOXO3 and MEF2A pathways. PMID: 27698266
  29. A lower FOXO3 mRNA expression in granulosa cells leads to poor oocyte development in patients with unexplained infertility undergoing controlled ovarian stimulation for in vitro fertilization-embryo transfer. PMID: 28621049
  30. Negative expression of FoxO3/FoxO4 and lymph node metastasis were the risk factors for the poor prognosis of bladder cancer. PMID: 28554751
  31. miR-155-5p promotes fibroblast cell proliferation and inhibits FOXO signaling pathway by negative modulation of both FOXO3 and CDKN1B in vulvar lichen sclerosis PMID: 29339071
  32. Cytoplasmic retention of FOXO3a may represent a potential biomarker for response to combined treatment with inhibitors of PI3K and autophagy in PIK3CA-mutant cervical cancer cells. PMID: 28036259
  33. Results show that FOXO3-phosphorylation at threonine-32 (T32) and nuclear localization in neuroblastoma biopsies significantly correlated with stage IV disease. Data suggest that, depending on the mode and intensity of activation, cellular FOXO3 acts as a homeostasis regulator promoting tumor growth at hypoxic conditions and tumor angiogenesis in high-stage neuroblastoma. PMID: 27769056
  34. FoxO3a might be a key regulator in cetuximab resistance through up-regulating c-Myc in colorectal cancer targeted therapy. PMID: 27825133
  35. Atorvastatin strengthens Skp2 binding to FOXO1 or ICAM1, leading to ubiquitination and degradation. Skp2-dependent ubiquitination of major pathogenic molecules is the key mechanism for statin's protective effect on endothelial function in diabetes. PMID: 28802579
  36. Transcriptional factor PAX3 (PAX3) exerted its tumor suppressor function by inhibiting the activity of major signaling pathways and enhancing expression and activity of transcription factor forkhead box O3 protein (FOXO3a). PMID: 27458157
  37. The result suggests that FOXO3 rs12212067 polymorphism does not play an important role in susceptibility to T. cruzi infection and/or chronic Chagas cardiomyopathy. PMID: 27125259
  38. Overexpression of circ-Foxo3 decreased the interaction between Foxo3 and MDM2, and repressed the function of MDM2 in modulating poly-ubiquitination of Foxo3. PMID: 27886165
  39. silencing FOXO3 diminishes bepridil- and trifluoperazine-induced apoptosis in triple-negative breast cancer cells PMID: 27283899
  40. The authors found that transient TUBB3 activation, through ABCB1, in response to the stimulation of FOXO3a expression, significantly contributes to the cross-resistance of the paclitaxel-resistant cell population and consequently limits the efficacy of both agents where cancer cells have developed multiple resistance. PMID: 27284014
  41. findings provide further evidence for the involvement of FoxO3 during terminal erythropoiesis and confirm the modulation of the PI3K/AKT pathway as a potential therapeutic strategy for beta-thalassemia PMID: 29099866
  42. The data reveal a previously unexplored function of FOXO3a in gastric cancer invasion by regulating proteins involved in extracellular matrix degradation and Epithelial-Mesenchymal Transition. FOXO3a may be of prognostic value and a potential therapeutic target in blocking tumor metastasis. PMID: 27127880
  43. pro-apoptotic role of miR-34a in PA-induced cholangiocyte lipoapoptosis in culture and in the liver PMID: 28250026
  44. Ergosterol peroxide stimulated Foxo3 activity by inhibiting pAKT and c-Myc and activating pro-apoptotic protein Puma and Bax to induce HepG2 cancer cell death. PMID: 27058618
  45. These results show that significantly increased levels of FOXO3, IRF4, and xIAP mRNA in Chinese HIV-1-infected patients. PMID: 27841661
  46. Knockdown of MIEF2 reduces DOX-induced mitochondrial fission and apoptosis in cardiomyocytes and in vivo. Also, knockdown of MIEF2 protects heart from DOX-induced cardiotoxicity. Our study identifies a novel pathway composed of Foxo3a and MIEF2 that mediates DOX cardiotoxicity. PMID: 28137654
  47. data show that the GSK3B-FOXO3 pathway is activated after partial hepatectomy, and this may be one of the mechanisms that lead to upregulation of hepatic IGF1R after partial hepatectomy. PMID: 28952285
  48. Chromosome 6q deletion correlates with poor prognosis and low relative expression of FOXO3 in chronic lymphocytic leukemia patients. PMID: 28699185
  49. Diabetic Glc also promoted beta-catenin nuclear localization and the formation of a complex with FOXO3a that localized to the promoters of Sod2, p21(cip1), and potentially p27(kip1) PMID: 27411103
  50. first report of the association between rs13217795 and allergic rhinitis, and the first independent verification of the association between rs13217795 and asthma PMID: 29141605

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Involvement in disease A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with KMT2A/MLL1.
Subcellular Location Cytoplasm, cytosol, Nucleus
Tissue Specificity Ubiquitous.
Database Links

HGNC: 3821

OMIM: 602681

KEGG: hsa:2309

STRING: 9606.ENSP00000339527

UniGene: Hs.220950


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