Phospho-FOXO3 (S253) Antibody

Datasheet

Code CSB-RA008836A253phHU
Size US$350
Uniprot No. O43524
Abbreviation Forkhead box protein O3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Western Blot
    Positive WB detected in:Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate
    All lanes:Phospho-FOXO3 antibody at 0.96ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 97 KDa
    Observed band size: 97 KDa

  • IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen A synthesized peptide derived from human Phospho-FOXO3 (S253)
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200
Relevance Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Forkhead box protein O3, AF6q21 protein, FOXO3
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Epigenetics and Nuclear Signaling
Gene Names FOXO3
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Function Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC
Involvement in disease A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with KMT2A/MLL1.
Subcellular Location Cytoplasm, cytosol, Nucleus
Tissue Specificity Ubiquitous.
Database Links

HGNC: 3821

OMIM: 602681

KEGG: hsa:2309

STRING: 9606.ENSP00000339527

UniGene: Hs.220950

Pathway Chemokine signaling pathway
PI3K-Akt signaling pathway
Cellular senescence
MAPK Erk pathway
AMPK signaling
FoxO signaling pathway
Neurotrophin signaling pathway

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