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| Code | CSB-YP007511HU |
| Product Type | Recombinant Protein |
| Size | US$2010 |
| Uniprot No. | P19525 |
| Relevance | IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin. |
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| Storage Buffer | Tris-based buffer,50% glycerol |
| Alias | Eukaryotic translation initiation factor 2-alpha kinase 2 Short name: eIF-2A protein kinase 2 Interferon-inducible RNA-dependent protein kinase P1/eIF-2A protein kinase Protein kinase RNA-activated Short name: PKR Short name: Protein kinase R1 Publication Tyrosine-protein kinase EIF2AK2 (EC:2.7.10.2) p68 kinase |
| Species | Homo sapiens (Human) |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Sequence | AGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC |
| Research Area | Signal Transduction |
| Source | Yeast |
| Gene Names | EIF2AK2 |
| Protein Names | Recommended name: Interferon-induced, double-stranded RNA-activated protein kinase EC= 2.7.11.1 Alternative name(s): Eukaryotic translation initiation factor 2-alpha kinase 2 Short name= eIF-2A protein kinase 2 Interferon-inducib |
| Expression Region | 2-551aa |
| Tag Info | N-terminal 6xHis-tagged |
| Mol. Weight | 64.0kDa |
| Protein Description | Full Length of Mature Protein |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
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| Function | IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin. |
| Subcellular Location | Cytoplasm, Nucleus, Cytoplasm, perinuclear region |
| Protein Families | Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily |
| Tissue Specificity | Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcin |
| Database Links |
HGNC: 9437 OMIM: 176871 KEGG: hsa:5610 STRING: 9606.ENSP00000233057 UniGene: Hs.131431 |
| Pathway |
Necroptosis
Protein processing in endoplasmic reticulum |
EIF2AK2 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA007511LD01HU | EIF2AK2 Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA007511LC01HU | EIF2AK2 Antibody, FITC conjugated | Human | ELISA |
| CSB-PA007511LB01HU | EIF2AK2 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA007511GA01HU | EIF2AK2 Antibody | Human | ELISA,WB,IHC,IF |
| CSB-PA572393 | Phospho-EIF2AK2 (Thr446) Antibody | Human,Mouse | ELISA,WB,IHC,IF |
| CSB-PA035578 | Phospho-EIF2AK2 (Thr451) Antibody | Human,Mouse | ELISA,IHC |
| CSB-PA168179 | EIF2AK2 (Ab-446) Antibody | Human | ELISA,WB,IHC,IF |
| CSB-PA028534 | EIF2AK2 (Ab-451) Antibody | Human,Mouse | ELISA,IHC,IF |
| CSB-PA003809 | EIF2AK2 Antibody | Human | WB,IHC,ELISA |
| CSB-PA040003 | Phospho-EIF2AK2 (T446) Antibody | Human | WB,ELISA |
| CSB-PA040004 | Phospho-EIF2AK2 (T451) Antibody | Human | IHC,ELISA |
| CSB-PA040005 | Phospho-EIF2AK2 (T258) Antibody | Human | WB,IHC,ELISA |
| CSB-PA040006 | EIF2AK2 Antibody | Human | WB,IHC,ELISA |
| CSB-PA040007 | EIF2AK2 Antibody | Human | IHC,ELISA |
| CSB-PA826977 | EIF2AK2 Antibody | Human | ELISA,IHC |
| CSB-RA007511A446phHU | Phospho-EIF2AK2 (T446) Antibody | Human | ELISA, WB, IHC, IF |
| CSB-PA007511LA01HU | EIF2AK2 Antibody | Human | ELISA, IHC, IF |
EIF2AK2 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-EP007511HU | Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2) | E.coli |
| CSB-BP007511HU CSB-MP007511HU |
Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2) | Baculovirus Mammalian cell |
Recombinant Saccharomyces cerevisiae Nicotinamidase(PNC1)
Express system: E.coli
Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Recombinant Human High affinity immunoglobulin epsilon receptor subunit alpha(FCER1A),partial
Express system: Yeast
Species: Homo sapiens (Human)
Recombinant Human Receptor-type tyrosine-protein phosphatase beta(PTPRB),partial
Express system: Yeast
Species: Homo sapiens (Human)
Recombinant Human Bisphosphoglycerate mutase(BPGM)
Express system: E.coli
Species: Homo sapiens (Human)
Recombinant Human T-cell surface glycoprotein CD8 alpha chain(CD8A),partial
Express system: Yeast
Species: Homo sapiens (Human)
Recombinant Mouse Thy-1 membrane glycoprotein(Thy1)
Express system: E.coli
Species: Mus musculus (Mouse)
