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| Code | CSB-RA007511A446phHU |
| Size | US$350 |
| Uniprot No. | P19525 |
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| Immunogen | A synthesized peptide derived from Human Phospho-EIF2AK2 (T446) |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| Relevance | IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin. |
| Form | Liquid |
| Conjugate | Non-conjugated |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method | Affinity-chromatography |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Alias | Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, Protein kinase RNA-activated, PKR, Protein kinase R, EIF2AK2, PKR, PRKR |
| Immunogen Species | Homo sapiens (Human) |
| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol |
| Research Area | Signal Transduction |
| Gene Names | EIF2AK2 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
| Function | IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin. |
| Subcellular Location | Cytoplasm, Nucleus, Cytoplasm, perinuclear region |
| Protein Families | Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily |
| Tissue Specificity | Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcin |
| Database Links |
HGNC: 9437 OMIM: 176871 KEGG: hsa:5610 STRING: 9606.ENSP00000233057 UniGene: Hs.131431 |
| Pathway |
Necroptosis
Protein processing in endoplasmic reticulum |
EIF2AK2 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA007511LD01HU | EIF2AK2 Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA007511LC01HU | EIF2AK2 Antibody, FITC conjugated | Human | ELISA |
| CSB-PA007511LB01HU | EIF2AK2 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA007511GA01HU | EIF2AK2 Antibody | Human | ELISA,WB,IHC,IF |
| CSB-PA572393 | Phospho-EIF2AK2 (Thr446) Antibody | Human,Mouse | ELISA,WB,IHC,IF |
| CSB-PA035578 | Phospho-EIF2AK2 (Thr451) Antibody | Human,Mouse | ELISA,IHC |
| CSB-PA168179 | EIF2AK2 (Ab-446) Antibody | Human | ELISA,WB,IHC,IF |
| CSB-PA028534 | EIF2AK2 (Ab-451) Antibody | Human,Mouse | ELISA,IHC,IF |
| CSB-PA003809 | EIF2AK2 Antibody | Human | WB,IHC,ELISA |
| CSB-PA040003 | Phospho-EIF2AK2 (T446) Antibody | Human | WB,ELISA |
| CSB-PA040004 | Phospho-EIF2AK2 (T451) Antibody | Human | IHC,ELISA |
| CSB-PA040005 | Phospho-EIF2AK2 (T258) Antibody | Human | WB,IHC,ELISA |
| CSB-PA040006 | EIF2AK2 Antibody | Human | WB,IHC,ELISA |
| CSB-PA040007 | EIF2AK2 Antibody | Human | IHC,ELISA |
| CSB-PA826977 | EIF2AK2 Antibody | Human | ELISA,IHC |
| CSB-PA007511LA01HU | EIF2AK2 Antibody | Human | ELISA, IHC, IF |
EIF2AK2 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP007511HU | Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2) | Yeast |
| CSB-EP007511HU | Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2) | E.coli |
| CSB-BP007511HU CSB-MP007511HU |
Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2) | Baculovirus Mammalian cell |
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