Tel : 301-363-4651
All
  • All
  • Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Raw Material For IVD Kit

Phospho-EIF2AK2 (T446) Antibody

Datasheet

Code CSB-RA007511A446phHU
Size US$350
Uniprot No. P19525
Abbreviation Interferon-induced, double-stranded RNA-activated protein kinase
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image

  • Western Blot
    Positive WB detected in：Hela whole cell lysate(treated with Calyculin A or not)
    All lanes：Phospho-EIF2AK2 antibody at 1.25ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 62 KDa
    Observed band size: 62 KDa

  • IHC image of CSB-RA007511A446phHU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells(treated with 50mM Calyculin A for 30min) with CSB-RA007511A446phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunogen A synthesized peptide derived from human Phospho-EIF2AK2 (T446)
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Relevance IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, Protein kinase RNA-activated, PKR, Protein kinase R, EIF2AK2, PKR, PRKR
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Gene Names EIF2AK2
Still Have Questions? Leave a Message or Start an on-line Chat
Function IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.
Subcellular Location Cytoplasm, Nucleus, Cytoplasm, perinuclear region
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
Tissue Specificity Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcin
Database Links

HGNC: 9437

OMIM: 176871

KEGG: hsa:5610

STRING: 9606.ENSP00000233057

UniGene: Hs.131431
Pathway Necroptosis
Protein processing in endoplasmic reticulum

Related Products

EIF2AK2 Antibody
EIF2AK2 Protein
EIF2AK2 cDNA

EIF2AK2 Antibodies

EIF2AK2 Antibodies for Homo sapiens (Human)

Code Product Name Species Reactivity Application
CSB-PA007511GA01HU EIF2AK2 Antibody Human ELISA,WB,IHC,IF
CSB-PA572393 Phospho-EIF2AK2 (Thr446) Antibody Human,Mouse ELISA,WB,IHC,IF
CSB-PA035578 Phospho-EIF2AK2 (Thr451) Antibody Human,Mouse ELISA,IHC
CSB-PA168179 EIF2AK2 (Ab-446) Antibody Human ELISA,WB,IHC,IF
CSB-PA028534 EIF2AK2 (Ab-451) Antibody Human,Mouse ELISA,IHC,IF
CSB-PA003809 EIF2AK2 Antibody Human WB,IHC,ELISA
CSB-PA040003 Phospho-EIF2AK2 (T446) Antibody Human WB,ELISA
CSB-PA040004 Phospho-EIF2AK2 (T451) Antibody Human IHC,ELISA
CSB-PA040005 Phospho-EIF2AK2 (T258) Antibody Human WB,IHC,ELISA
CSB-PA040006 EIF2AK2 Antibody Human WB,IHC,ELISA
CSB-PA040007 EIF2AK2 Antibody Human IHC,ELISA
CSB-PA826977 EIF2AK2 Antibody Human ELISA,IHC

EIF2AK2 Proteins

EIF2AK2 Proteins for Mus musculus (Mouse)

Code Product Name Source
CSB-YP007511MO Recombinant Mouse Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2),Yeast Yeast
CSB-EP007511MO Recombinant Mouse Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2),Escherichia Coli E.coli
CSB-BP007511MO Recombinant Mouse Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2),Baculovirus Baculovirus
CSB-MP007511MO Recombinant Mouse Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2),Mammalian cell Mammalian cell

EIF2AK2 Proteins for Homo sapiens (Human)

Code Product Name Source
CSB-YP007511HU Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2) Yeast
CSB-EP007511HU Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2) E.coli
CSB-BP007511HU Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2),Baculovirus Baculovirus
CSB-MP007511HU Recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2),Mammalian cell Mammalian cell

EIF2AK2 Proteins for Rattus norvegicus (Rat)

Code Product Name Source
CSB-YP723560RA Recombinant Rat Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2),Yeast Yeast
CSB-EP723560RA Recombinant Rat Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2),Escherichia Coli E.coli
CSB-BP723560RA Recombinant Rat Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2),Baculovirus Baculovirus
CSB-MP723560RA Recombinant Rat Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2),Mammalian cell Mammalian cell

EIF2AK2 cDNA

EIF2AK2 cDNA for Rattus norvegicus (Rat)

Code Product Name Vector Accession NO.
CSB-CL723560RA Eif2ak2 pUC NM_019335.1

EIF2AK2 cDNA for Mus musculus (Mouse)

Code Product Name Vector Accession NO.
CSB-CL007511MO Eif2ak2 pUC NM_011163.4

Most popular with customers

Product Center

Kit

Protein

Antibody

Molecular Biology Product

Small Molecule

Promotions
Star Products
+ Show More

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.