Recombinant Mouse Ephrin type-B receptor 4(Ephb4),partial

Code CSB-YP007732MO
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Source Yeast
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Code CSB-EP007732MO
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Source E.coli
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Code CSB-EP007732MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007732MO
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Source Baculovirus
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Code CSB-MP007732MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ephb4
Uniprot No. P54761
Alternative Names Ephb4; Htk; Mdk2; Myk1Ephrin type-B receptor 4; EC 2.7.10.1; Developmental kinase 2; mDK-2; Hepatoma transmembrane kinase; Tyrosine kinase MYK-1
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.
Gene References into Functions
  1. These findings implicate a molecular disinhibitory mechanism driving the establishment of perisomatic inhibition whereby visual experience enhances Pten signalling, resulting in the suppression of EphB4 expression; this relieves a native synaptic repulsion between PV cells and pyramidal neurons, thereby promoting the assembly of perisomatic inhibition. PMID: 27611660
  2. EPHB4 is a critical regulator of early lymphatic vascular development; mutations in the gene can cause an autosomal dominant form of LRHF that is associated with a high mortality rate PMID: 27400125
  3. These results identify EPHB4/ephrin B2 signaling as critical to Hematopoietic stem and progenitor cells mobilization from bone marrow PMID: 27820703
  4. Eph-B4 stimulates eNOS phosphorylation in vitro and may mediate vein graft adaptation by regulation of eNOS activity in vivo. PMID: 26817610
  5. Its overexpression causes cisplatin resistant in melanomas. PMID: 26390965
  6. EphB4 promotes endochondral ossification while inhibiting osteoclast development during callus formation. PMID: 23165754
  7. Selective inhibition of EphB4 using a functional blocking antibody results in defective lymphatic valve development. PMID: 25865237
  8. Overexpression of bone-specific Ephb4 clearly demonstrated prevention of the development and/or progression of fibrosis in OA synovial membrane PMID: 25453723
  9. EphB4-forward signaling plays a crucial role in the development of cardiac progenitor cells. PMID: 25359705
  10. EPHB4 plays an essential role in regulating small artery contractility and blood pressure PMID: 25903126
  11. the luminal epithelial EphB4 signaling contributes, most likely via wnt signaling, to the regulation of migration and cell fate of early progenitors and is involved in the determination of branching points along the mammary ductal tree PMID: 24635767
  12. thymocytes develop normally in the absence of EPHB4, and T cells derived from EPHB4-deleted thymic epithelian cells have normal function. PMID: 24246266
  13. These results are the first to identify EphB4 and its cross-talk with PDGFRbeta as unexpected vital determinants of tumor cell survival in alveolar rhabdomyosarcoma. PMID: 24733895
  14. Identify EphB4 signaling as a pathway allowing podocytes to survive transient capillary collapse during glomerular disease. PMID: 22398409
  15. negative inhibitor of some VEGF-A functions in adult venous endothelial cells PMID: 23394931
  16. These findings suggest that EphB4 remains active in adult venous endothelial cells and that loss of EphB4 plays a role in vein graft adaptation. PMID: 23269240
  17. Bone-specific EphB4 overexpression exerts a protective effect on osteoarthritis joint structural changes. PMID: 22833446
  18. MAb47 targets fibronectin-like domain 2 of both human and murine EphB4 and does not alter EphB4 receptor levels, but inhibits angiogenesis and growth of both EphB4-positive and EphB4-negative tumors in a mouse s.c. xenograft model PMID: 20133814
  19. Ephrin-B2-specific ephrin B4 receptor stimulation of HUAEC activates a kinase that phosphorylates murine GST-ephrin-B1 cytoplasmic domain fusion proteins. PMID: 11983165
  20. EphB4 reverse signaling differentially affects cell adhesion and migration between arterial and venous endothelial cells. PMID: 12588758
  21. EphB4 receptor tyrosine kinase may have a role in the development of glomerulopathies PMID: 12676328
  22. modifies the rate and magnitude of embryonic stem cells acquiring genotypic and phenotypic characteristics of mesodermal tissues PMID: 12958066
  23. These results suggest that activation of EphB4 participates in adhesive but not repulsive signals independently of its tyrosine kinase activity in hematopoietic cells. PMID: 15358160
  24. EphB4 acts as a negative regulator of blood vessel branching and vascular network formation, switching the vascularization program from sprouting angiogenesis to circumferential vessel growth. PMID: 16424904
  25. activation of EphB4 enhances proangiogenic capacity through induction of PSGL-1 expression and adhesion to E selectin and P selectin PMID: 17510705
  26. EphB4 overexpression in B16 tumor cells suppresses the survival of arterial ECs in tumors by a reverse signaling via ephrinB2. PMID: 17942910
  27. EphB4 is a critical component of the CYP2C9- and 11,12-EET-activated signaling cascade that promotes angiogenesis in vitro as well as in vivo. PMID: 18340006

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily
Tissue Specificity Expressed in various organ systems, including lung, liver, kidney, intestine, muscle and heart (PubMed:7478528). Expressed in myogenic progenitor cells (PubMed:27446912).
Database Links

KEGG: mmu:13846

STRING: 10090.ENSMUSP00000106684

UniGene: Mm.34533

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