Recombinant Mouse Guanine nucleotide-binding protein G (i) subunit alpha-2 (Gnai2)

In Stock Promotion
Code CSB-EP009589MO
Size $388
Order now
Promotional price as low as $99
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Greater than 90% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Signal Transduction
Alternative Names
Adenylate cyclase-inhibiting G alpha protein
Mus musculus (Mouse)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
44.4 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
Gene References into Functions
  1. In conclusion, Galphai2 deficiency in erythrocytes confers partial protection against suicidal cell death. PMID: 27499046
  2. This study reveals how RGS proteins modulate Galphai2 signaling to facilitate thymocyte egress and T cell trafficking. PMID: 28235863
  3. this study shows that Galphai2 controls arrest in response to chemokine stimulation of neutrophils PMID: 26976957
  4. In Galphai2-knockdown mice, they showed defects in social interaction, novelty recognition and active avoidance learning. PMID: 27787898
  5. Data show that inhibitory (Galphai2) and stimulatory (GalphasL) G-protein subunits produced minor atrophic and hypertrophic changes in muscle mass, and Galphai2 over-expression prevented AAV:beta2-adrenoceptor (beta2-AR) mediated hypertrophy. PMID: 26972746
  6. RGS proteins and Galphai2 play a significant role in regulating states of wakefulness, NREM sleep, and REM sleep PMID: 26564126
  7. Kinocilium is essential for proper localization of Lgn, as well as Gai and aPKC, suggesting that cilium function plays a role in positioning of apical proteins critical for hearing. PMID: 26662512
  8. Galphai2 deficiency combined with cardiac beta1-adrenoceptor overexpression strongly impaired survival and cardiac function, leading to dilated cardiomyopathy. PMID: 26464333
  9. An essential role for Galpha(i2) in Smoothened-stimulated epithelial cell proliferation in the mammary gland. PMID: 26373672
  10. Platelet Galphai2 not only controls hemostatic and thrombotic responses but also is critical for the development of ischemia/reperfusion injury in vivo. PMID: 25944935
  11. Data show that disrupting resistance to inhibitors of cholinesterase 8A (Ric-8A) expression in hematopoietic cells results in a loss of GTP-binding protein alpha subunits Galphai2, Galphai3, and Galphaq. PMID: 26232433
  12. Myocardial ischemia promptly regulated cardiac mRNA and with a slight delay protein levels of both Galphai2 and Galphai3, indicating important roles for both Galphai isoforms. PMID: 24858945
  13. RGS protein/Galphai2 interactions have an essential role in B lymphocyte-directed cell migration and trafficking. PMID: 25617475
  14. Data (including data from knockout mice) suggest that Gnai2 in islet beta-cells is necessary for up-regulation of insulin secretion in response to high glucose in presence of L-arginine or L-ornithine. PMID: 25205820
  15. The absence of Galphai2 and Galphai3 in B cells profoundly disturbs the architecture of lymphoid organs with loss of B cell compartments in the spleen, thymus, lymph nodes, and gastrointestinal tract. PMID: 23977324
  16. Galphai2(Q205L) regulates satellite cell differentiation into myotubes in a protein kinase C - and histone deacetylase -dependent manner PMID: 24298018
  17. Galphas- and Galphai2-mediated signaling in the sinoatrial node is important in the reciprocal regulation of heart rate through the autonomic nervous system. PMID: 23697798
  18. Galphai2-deficient mice spontaneously develop colitis and colonic neoplasms the severity of which are determined by the interplay between Th1 and Th2 cell pathways. PMID: 22962436
  19. Go2alpha protein subunit regulates axonal growth and branching and may increase Rap1GAP activity, thereby effecting the Rap1 activation/deactivation cycle. PMID: 23373526
  20. Galphai2 is required for endothelial neutrophil transmigration but not neutrophil chemotaxis and adhesion. PMID: 23225882
  21. These studies support our hypothesis that G-alpha(i2) proteins are novel negative regulators of Toll-like receptor activation. PMID: 22581266
  22. RGS proteins establish a threshold for Galpha(i2) activation, helping to coordinate desensitization mechanisms during inflammation. PMID: 22966200
  23. A quantitative study of the mechanisms behind thymic atrophy in Galphai2-deficient mice during colitis development. PMID: 22590596
  24. Galphai2 is an essential Galphai protein required for inflammation-induced migration of monocytes and macrophages in Galphai2- or Galphai3-deficient mice. PMID: 22706085
  25. analysis of Galphai2- and Galphai3-specific regulation of voltage-dependent L-type calcium channels in cardiomyocytes PMID: 21966394
  26. Demonstrate that signalling through Gai2 protects the heart from ischaemic injury and that this process is modulated by endogenous RGS proteins. PMID: 21349876
  27. Cecal ligation and puncture -induced plasma TNFalpha, IL-6, bacterial load in peritoneal fluid, blood and lung tissue and lung and liver MPO activity were significantly increased in Galphai2 (-/-) compared to WT mice. PMID: 21255617
  28. Variations in Gnai2 and Rgs1 expression affect chemokine receptor signaling and the organization of secondary lymphoid organs. PMID: 20508603
  29. Data suggest that Galphai-TNFAIP8-mediated rescue of pre-oncogenic cells enhances progression to oncogenic transformation, providing a selective target to inhibit cellular transformation. PMID: 20607800
  30. The absence of inhibitory G-protein signaling mediated through Galpha(i2) is a substrate for ventricular arrhythmias. PMID: 20495013
  31. G alpha i2(-/-) mice are deficient in B-1a B cells, splenic marginal zone B cells, and their immediate precursor, T2 B cells, with resultant in vitro functional deficiency in LPS-induced proliferation and IL-10 production. PMID: 12574334
  32. Gi2 has a role in regulating mitogenic activity in the neural progenitor cells of developing brain PMID: 15272018
  33. Alveolar macrophages use a Galphai2-dependent signal for modulating the two opposing Fc gamma receptors, FcgammaRIIB and FcgammaRIII, in the initiation of the inflammatory cascade in the lung Arthus reaction. PMID: 15728518
  34. Constitutively active Galphai2 occludes the transient suppression of synaptic transmission by mGluR2. PMID: 16606834
  35. Loss of Regulators of G protein signaling (RGS) actions at Galpha(i2) produces a dramatic and pleiotropic phenotype which is more evident than the phenotype seen for individual RGS protein knockouts. PMID: 16943428
  36. These data demonstrate that G(i2) and G(i1/3) proteins are both involved and differentially regulate splenocyte inflammatory cytokine and chemokine production in a highly Gi isoform specific manner in response to LPS and Gram-positive microbial stimuli. PMID: 16962188
  37. Galphai2 signaling events are not required for the development of the protective immune responses against S. stercoralis; however, Galphai2 is essential for the recruitment of neutrophils required for host-dependent killing of larvae. PMID: 17242370
  38. This study details distinct G alpha(i2) and G alpha(i3) effects on chemokine receptor CXCR3-mediated signaling. PMID: 17289675
  39. Gnai2 knockout mice show significantly reduced intestinal IL-11 production. Suggest MAP kinases, PGE2 and cAMP mediated signaling involved. PMID: 17332478
  40. specific Galpha(i2)-mediated signaling between endothelial cells and leukocytes is required for the extravasation of leukocytes and for tissue-specific accumulation PMID: 17360531
  41. Ghrelin attenuates glucose-induced insulin release via pertissigen-sensitive Galpha(i2)-mediated activation of potassium channels. PMID: 17575083
  42. Gnai2-deficient T cells exhibit significant defects in chemokine receptor signaling and illustrate how signals that modulate the level of Galphai2 present in lymphocytes directly affect the capacity of lymphocytes to respond to chemokines. PMID: 17579064
  43. Neutrophil Galpha(i2) is necessary for chemokine-induced arrest. PMID: 17699741
  44. Transgenic mice on a high fat diet which have this gene exhibit enhanced insulin sensitivity and increased glucose tolerance. PMID: 17928396
  45. results indicate that the upregulation of Gi2 in ischemic myocardium appears to be protective in inducing cell survival pathways to prevent myocyte death in response to ischemia/reperfusion injury PMID: 18316484
  46. Absence of either Galphai2 or Galphai3 significantly abrogated thymic homing, with an effect of Galphai3 being greater than that of Galphai2. PMID: 18501427
  47. These observations underscore interplay between Galphai2 and Galphai3 and potentially provide a novel strategy to prevent GVHD by blocking T cell homing at early stages and T effector cell trafficking at later time points. PMID: 18521956
  48. Report role of Gnai2 in the control of in vivo heart rate dynamics. PMID: 18832081
  49. In Galphai2(-/-) and interleukin-10(-/-) transgenic mice susceptible to immune-mediated colitis and inflammation-associated adenocarcinoma, similar levels of peripheral leukocyte and erythroblast genotoxicity were also observed. PMID: 19487293
  50. Young Gialpha2-/- colonic epithelium has normal MMR expression but selectively loses MLH1 and consequently PMS2 expression following inflammation. PMID: 19638594

Show More

Hide All

Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell membrane. Membrane; Lipid-anchor.
Protein Families
G-alpha family, G(i/o/t/z) subfamily
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join Us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Place an order now

I. Product details


II. Contact details


III. Ship To


IV. Bill To