Recombinant Mouse Protein SGT1 homolog (Ecd)

Code CSB-YP875134MO
MSDS
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Source Yeast
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Code CSB-EP875134MO
MSDS
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Source E.coli
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Code CSB-EP875134MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875134MO
MSDS
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Source Baculovirus
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Code CSB-MP875134MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ecd
Uniprot No.
Alternative Names
EcdProtein ecdysoneless homolog
Species
Mus musculus (Mouse)
Expression Region
1-641
Target Protein Sequence
MEGSGKLAMV EDAVEYHLFL IPDKARGTEE HREILQKYIE RIMTQFAPIL VPYIWQNQPF NLKYKPAKGG VPAHMYGMTK FGDNIEDEWF IVYVIKQITK EFPELVARVE DNDGEFLLIE AADFLPKWLD PDNSANRVFF HHGELCIIPV PRKSERIPWL PMTPPTIQQA LSIISAHPEA VLASESIQAA VDRRVSGYPE RVEASLHRAH CFLPAGIVAV LKQQPRLLSA AVQAFYLRDP IDLRACRVFK TFLPETRIMA SVTFTKCLYA QLVQQKFVPD RRSGYGLPPP SHPQYRAYEL GMKLAHGFEI LCSKCSPHFS DSRKSLVTAS PLWASFLESL KRNDYFKGLM DGSAQYQERL EMAKNYFQLS IHRPESSLAM SPGEEILTVL QTQPFDVAEL KTEEADLPPE DDDQWLDLSP DQLDQLLQDA AGRKESQPGP QKEELQNYDV AQVSDSMKAF ISKVSSHKGA ELPRDPSEAP ITFDADSFLN YFDKILGAKP QESDSEDDPG EEDVEGVDSD DDVGFEAQES ESLKGALGSL KSYMARMDQE LAHTSMGRSF TTRERLNKDP PSHTANDNSD EEDSGAGDCA VEAVDVDLNL ISNILESYSS QAGLAGPASN LLHSMGVRLP DNADHNPQVS Q
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.
Gene References into Functions
  1. The data demonstrate that ECD promotes survival upon endoplasmic reticulum stress by increasing GRP78 protein levels to enhance the adaptive folding protein in the endoplasmic reticulum to attenuate PERK signaling. PMID: 28652267
  2. Results demonstrate that mammalian Ecd plays a role in cell cycle progression via the Rb-E2F pathway. PMID: 19640839
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
ECD family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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