Recombinant Mouse Transcriptional activator protein Pur-alpha (Pura)

Code CSB-YP019083MO
MSDS
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Source Yeast
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Code CSB-EP019083MO
MSDS
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Source E.coli
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Code CSB-EP019083MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019083MO
MSDS
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Source Baculovirus
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Code CSB-MP019083MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pura
Uniprot No.
Alternative Names
Pura; Transcriptional activator protein Pur-alpha; Purine-rich single-stranded DNA-binding protein alpha
Species
Mus musculus (Mouse)
Expression Region
1-321
Target Protein Sequence
MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGAP GGLQHETQEL ASKRVDIQNK RFYLDVKQNA KGRFLKIAEV GAGGNKSRLT LSMSVAVEFR DYLGDFIEHY AQLGPSQPPD LAQAQDEPRR ALKSEFLVRE NRKYYMDLKE NQRGRFLRIR QTVNRGPGLG STQGQTIALP AQGLIEFRDA LAKLIDDYGV EEEPAELPEG TSLTVDNKRF FFDVGSNKYG VFMRVSEVKP TYRNSITVPY KVWAKFGHTF CKYSEEMKKI QEKQREKRAA CEQLHQQQQQ QQEETTAATL LLQGEEEGEE D
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the c-Myc gene. May play a role in the initiation of DNA replication and in recombination.
Gene References into Functions
  1. Quantitative immunohistochemical assays of the vermal region of the cerebellum and CA1-3 regions of the hippocampus revealed reduced numbers of neurons in general, as well as reduced numbers of Pur-alpha+-immunopositive neurons and dendrites in heterozygous Pur-alpha mice, compared to wild-type littermates PMID: 27651147
  2. This study underlines the importance of Puralpha in the proliferation of neuronal precursor cells during postnatal brain development and suggest a role for Puralpha in the regulation of the expression and cellular distribution of dendritic and axonal proteins. PMID: 22010047
  3. Data suggest that Puralpha has a role in nucleotide excision repair and the repair of UVC-induced DNA damage. PMID: 20948313
  4. Overexpression of Purbeta, but not Puralpha, significantly downregulated GnRH1 mRNA Levels in transiently transfected GT1-7 cells, suggesting that Purbeta acts as a repressor of GnRH1 gene transcription. PMID: 19996387
  5. Identification of mrna/protein (mRNA) complexes and their association with rough endoplasmic reticulum equipped with a kinesin motor PMID: 12147688
  6. Pura is essential for postnatal brain development and developmentally coupled cellular proliferation as revealed by genetic inactivation. PMID: 12972605
  7. we also determined a region in Pur alpha which is required for the association with the purine rich element binding protein by using deletion mutants of Pur alpha PMID: 15234119
  8. Puralpha has been shown to colocalize with cyclin A/Cdk2 and to coimmunoprecipitate with cyclin A during S-phase and we show that this interaction is mediated by a specific affinity of Puralpha for Cdk2. PMID: 15707957
  9. analysis of of DNA binding and localized strand separation by Pur alpha and comparison with Pur beta PMID: 15777841
  10. Pur alpha and Pur beta repress smooth muscle alpha-actin gene transcription by means of DNA strand-selective cis-element binding and cell type-dependent protein-protein interactions PMID: 16436378
  11. E2F-1 and Puralpha interplay appears to be involved in the regulation of Puralpha expression and the cell cycle. PMID: 16741925
  12. These data demonstrate that Pur proteins collaborate with Sp3 to regulate a transcriptional program that enables muscle cells to remodel their phenotype. PMID: 17145772
  13. Activation of the normally silent SM alpha A gene in reprogrammed adult cardiomyocytes is linked to elevated interaction of serum response factor with Pur-alpha/Pur-beta. PMID: 18344281
  14. Expression patterns suggest an accessory role of C9orf10 for Puralpha in a limited brain region in addition to other possible functions that have not yet been determined. PMID: 18413649
  15. Tat stimulates the Rad51 promoter involving both Puralpha-dependent and Puralpha-independent mechanisms. Interaction between Puralpha and Tat may have opposing effects on Rad51 expression. PMID: 18630497
  16. analysis of how a bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Pur alpha) PMID: 19720825

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Subcellular Location
Nucleus.
Protein Families
PUR DNA-binding protein family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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