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The production of the ATF2 recombinant monoclonal antibody involves the utilization of DNA recombinant technology and in vitro genetic manipulation. Initially, an animal is immunized with a synthesized peptide derived from human ATF2, and B cells are isolated for further screening. Positive B cells are selected, and individual clones are identified. The light and heavy chains of the ATF2 antibody are then amplified using PCR and inserted into a plasmid vector to create a recombinant vector. This vector is transfected into a host cell line to enable the expression of the antibody. The ATF2 recombinant monoclonal antibody is subsequently purified from the cell culture supernatant using affinity chromatography. This antibody specifically recognizes human ATF2 protein and is recommended for use in ELISA, WB, and IHC applications.
ATF2 is a transcription factor that regulates the expression of many genes in response to various cellular stress stimuli, including UV irradiation, osmotic shock, and inflammatory cytokines. In cells, ATF2 protein can bind to DNA and activate the transcription of genes involved in cell growth, differentiation, and apoptosis. ATF2 also plays a critical role in the cellular response to stress by activating the MAP kinase signaling pathway and promoting the expression of stress-responsive genes. It is involved in regulating cell proliferation, survival, and differentiation, and has been implicated in various diseases, including cancer and neurodegeneration.
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