ATF2 Antibody

Code CSB-RA002270A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate
    All lanes: ATF2 antibody at 1.2μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 55, 36, 24, 49, 53, 14, 25, 16 KDa
    Observed band size: 70 KDa

  • IHC image of CSB-RA002270A0HU diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Uniprot No. P15336
Target Names ATF2
Alternative Names Activating transcription factor 2 antibody; Activating transcription factor 2 splice variant ATF2 var2 antibody; ATF 2 antibody; Atf-2 antibody; Atf2 antibody; ATF2 protein antibody; ATF2_HUMAN antibody; cAMP Response Element Binding Protein 2 antibody; cAMP response element binding protein CRE BP1 antibody; cAMP response element-binding protein CRE-BP1 antibody; cAMP responsive element binding protein 2; formerly antibody; cAMP-dependent transcription factor ATF-2 antibody; cAMP-responsive element-binding protein 2 antibody; CRE BP1 antibody; CRE-BP antibody; CREB 2 antibody; CREB-2 antibody; CREB2 antibody; CREBP1 antibody; Cyclic AMP dependent transcription factor ATF 2 antibody; Cyclic AMP-dependent transcription factor ATF-2 antibody; Cyclic AMP-responsive element-binding protein 2 antibody; D130078H02Rik antibody; D18875 antibody; HB 16 antibody; HB16 antibody; Histone acetyltransferase ATF2 antibody; MGC105211 antibody; MGC105222 antibody; MGC111558 antibody; MGC142504 antibody; mXBP antibody; MXBP protein antibody; Tg(Gzma-Klra1)7Wum antibody; TREB 7 antibody; TREB7 antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from human ATF2
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
Gene References into Functions
  1. Our study found that miR-451 regulates the drug resistance of renal cell carcinoma by targeting ATF-2 PMID: 28429654
  2. deregulation of the miR-144-5p/ATF2 axis plays an important role in non-small-cell lung cancer cell radiosensitivity. PMID: 29850528
  3. p38alpha and ATF2 expression play a crucial role in the malignant phenotypes of ovarian tumor cells and are a markers of poor prognosis in patients with ovarian serous adenocarcinomas. PMID: 28916425
  4. activation of JNK was found to be dependent on muscarinic acid receptor induced Ca(2+)/CAMKII as well as ROS. JNK dependent phosphorylation of ATF2/c-Jun transcription factors resulted in TGF-beta transcription and its signaling. PMID: 27708346
  5. ATF2 regulated by miR-204 might also play an important role in the regulation of malignant behavior of glioblastoma. PMID: 27588402
  6. We further demonstrated the suppressive function of lncRNA#32 in hepatitis B virus and hepatitis C virus infection. lncRNA#32 bound to activating transcription factor 2 (ATF2) and regulated ISG expression. Our results reveal a role for lncRNA#32 in host antiviral responses. PMID: 27582466
  7. Results show that ATF2 is highly expressed in renal cell carcinoma (RCC) tissues and promotes RCC cell proliferation, migration and invasion. The study suggests that ATF2 exerts an oncogenic role in RCC. PMID: 27377902
  8. These findings point to an oncogenic function for ATF2 in melanoma development that appears to be independent of its transcriptional activity. PMID: 27210757
  9. this study demonstrates that CPEB2 alternative splicing is a major regulator of key cellular pathways linked to anoikis resistance and metastasis. PMID: 28904175
  10. Noxin facilitated the expression of Cyclin D1 and Cyclin E1 through activating P38-activating transcription factor 2 signaling pathway, thus enhanced cell growth of breast cancer PMID: 28618963
  11. these observations suggest that CD99 is involved in the regulation of CD1a transcription and expression by increasing ATF-2. PMID: 27094031
  12. This review provides an overview of the currently known upstream regulators and downstream targets of ATF2. [review] PMID: 28212892
  13. TNF induces the binding of ATF2 to the TNF-responsive element. PMID: 27821620
  14. miR-204 may act as a tumor suppressor by directly targeting ATF2 in non-small cell lung cancer PMID: 26935060
  15. the variant alleles of TSG101 rs2292179 and ATF2 rs3845744 were associated with a reduced risk of breast cancer, particularly for subjects with BMI <24 (kg/m(2)) and postmenopausal women, respectively PMID: 26729199
  16. Results reveal that mitochondrial ATF2 is associated with the induction of apoptosis and BRAF inhibitor resistance through Bim activation. PMID: 26462148
  17. Neisseria meningitidis caused a high level of E-selectin expression elicited by the activity of phosphorylated ATF2 transcription factor on the E-selectin promoter. PMID: 26153406
  18. increased expression of the gene encoding PKCepsilon and abundance of phosphorylated, transcriptionally active ATF2 were observed in advanced-stage melanomas and correlated with decreased FUK expression PMID: 26645581
  19. CARMA1- and MyD88-dependent activation of Jun/ATF-type AP-1 complexes is a hallmark of ABC diffuse large B-cell lymphomas. PMID: 26747248
  20. More terminally differentiated human odontoblasts was ATF-2 positive, as compared to pulpal fibroblasts at various stages of differentiation: ATF-2 is more associated with cell survival rather than cell proliferation. PMID: 25417007
  21. Study identified a potential target of miR-451, ATF2, and revealed a novel role of miR-451 in the inhibition of the migratory ability of hepatoma cell lines. PMID: 24968707
  22. ATF-2 knockdown blocked VEGF-A-stimulated VCAM-1 expression and endothelial-leukocyte interactions. ATF-2 was also required for other endothelial cell outputs, such as cell migration and tubulogenesis. PMID: 24966171
  23. Study demonstrates the role of miR-622 in suppressing glioma invasion and migration mediated by ATF2, and shows that miR-622 expression inversely correlates with ATF2 in glioma patients PMID: 25258251
  24. suppression of tumorigenesis by JNK requires ATF2. PMID: 25456131
  25. study revealed that, autocrine soluble factors regulate dual but differential role of ATF-2 as a transcription factor or DNA repair protein, which collectively culminate in radioresistance of A549 cells. PMID: 25041846
  26. While expression of ATF-2 is not associated with outcome. PMID: 25141981
  27. The expression of ATF2 in chondrocytes is involved in apoptosis in Kashin-Beck disease. PMID: 23866832
  28. in human HCC tissues, SPTBN1 expression correlated negatively with expression levels of STAT3, ATF3, and CREB2; SMAD3 expression correlated negatively with STAT3 expression PMID: 25096061
  29. zymosan-induced il23a mRNA expression is best explained through coordinated kappaB- and ATF2-dependent transcription; and (iii) il23a expression relies on complementary phosphorylation of ATF2 on Thr-69 and Thr-71 dependent on PKC and MAPK activities. PMID: 24982422
  30. Data show that salvianolic acid B protects endothelial progenitor cells against oxidative stress by modulating Akt/mTOR/4EBP1, p38 MAPK/ATF2, and ERK1/2 signaling pathways. PMID: 24780446
  31. There is synergism between developmental stage-specific recruitments of the ATF2 protein complex and expression of gamma-globin during erythropoiesis. PMID: 24223142
  32. An association between ATF2 polymorphisms and heavy alcohol consumption is only weakly supported. PMID: 24338393
  33. ATF2 knockdown revealed ATF2-triggered p21(WAF1) protein expression, suggesting p21(WAF1) transactivation through ATF2. PMID: 23800081
  34. results therefore suggest that c-MYC induces stress-mediated activation of ATF2 and ATF7 and that these transcription factors regulate apoptosis in response to oncogenic transformation of B cells PMID: 23416976
  35. we establish that ATF2 family members physically and functionally interact with TCF1/LEF1 factors to promote target gene expression and hematopoietic tumor cell growth PMID: 23966864
  36. Cytoplasmic ATF2 expression was less frequently seen than nuclear expression in malignant mesenchymal tumors. Benign mesenchymal tumors mostly showed much lower nuclear and cytoplasmic ATF2 expression. PMID: 24289970
  37. Data indicate that small molecules that block the oncogenic addiction to PKCepsilon signaling by promoting ATF2 nuclear export, resulting in mitochondrial membrane leakage and melanoma cell death. PMID: 23589174
  38. Increasing of ATF2 expression is mediated via oxidative stress induced by arsenic in SV-HUC-1 cells, and MAPK pathways are involved. PMID: 23591579
  39. these studies show that the IL-1beta-induced increase in intestinal tight junction permeability was regulated by p38 kinase activation of ATF-2 and by ATF-2 regulation of MLCK gene activity PMID: 23656735
  40. Phosphorylation of ATF2 by PKCepsilon is the master switch that controls its subcellular localization and function. PMID: 22685333
  41. ATF2-Jun heterodimers bind IFNb in both orientations alone and in association with IRF3 and HMGI PMID: 22843696
  42. we report the kinetic mechanism for JNK1beta1 with transcription factors ATF2 and c-Jun along with interaction kinetics for these substrates. PMID: 22351776
  43. ATF2 subcellular localization is probably modulated by multiple mechanisms PMID: 22275354
  44. Data concluded that IR-induced up-regulation of ATF2 was coordinately enhanced by suppression of miR-26b in lung cancer cells, which may enhance the effect of IR in the MAPK signaling pathway. PMID: 21901137
  45. The ability of ATF2 to reach the mitochondria is determined by PKCepsilon, which directs ATF2 nuclear localization. Genotoxic stress attenuates PKCepsilon effect on ATF2; enables ATF2 nuclear export and localization at the mitochondria. PMID: 22304920
  46. Data show that ATF7-4 is an important cytoplasmic negative regulator of ATF7 and ATF2 transcription factors. PMID: 21858082
  47. Our data suggest regulatory roles for ATF2 in TNF-related mechanisms of Head and Neck Squamous Cell Carcinoma. Its perturbation and nuclear activation are associated with significant effects on survival and cytokine production. PMID: 21990224
  48. Data suggest that competition between GSTpi and active JNK for the substrate ATF2 may be responsible for the inhibition of JNK catalysis by GSTpi. PMID: 21384452
  49. ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
  50. MITF is downregulated by ATF2 in the skin of Atf2-/- mice, in primary human melanocytes, and in melanoma cell lines. PMID: 21203491
  51. hyperosmotic stress can activate the Plk3 signaling pathway that subsequently regulates the AP-1 complex by directly phosphorylating ATF-2 independent from the effects of JNK and p38 activation. PMID: 21098032
  52. calorimetric studies of atf-2 and c-jun transcription factors PMID: 20619956
  53. Results suggest that Fra-2 protein may be more effective than ATF-2 protein in cyst formation originated from epithelial cells of dental follicles. PMID: 20675274
  54. rapid phosphorylation of p38 mitogen-activated protein kinase and activating transcription factor 2 also takes place in IM-9 lymphoblastoid cells, which naturally express GPR55, after stimulation with LPI PMID: 20051382
  55. Overexpression of ATF2 resulted in significant increase in ATF3 promoter activity, and electrophoretic mobility shift assay identified this region as a core sequence to which ATF2 binds PMID: 20581861
  56. Growth factor-independent signaling pathways converge in the formation of an active c-Jun.AFT2 dimer, which induces the expression of the anti-apoptotic factor Bcl-X(L) that mediates a pro-survival response. PMID: 20507983
  57. JNK/ATF2 pathway is involved in iodinated contrast media-induced apoptosis PMID: 19923798
  58. JNK-ATF-2 inhibits thrombomodulin (TM) expression by recruiting histone deacetylase4 (HDAC4) and forming a transcriptional repression complex in the TM promoter. PMID: 20116378
  59. Stability and DNA-binding ability of the bZIP dimers formed by the ATF-2 and c-Jun. PMID: 19944700
  60. Infrequent mutations of the activating transcription factor-2 gene in human lung cancer, neuroblastoma and breast cancer PMID: 11836564
  61. activation by growth factors via phosphorylation of Thr71 through the Ras-MEK-ERK pathway and of Thr69 through RalGDS-Src-p38 PMID: 12110590
  62. Phosphorylation of one of the downstream transcriptional factors of MAPK cascade, ATF2, was 3.2- and 2.0-fold induced by TPA and Saikosaponin a, respectively. PMID: 12592382
  63. JNK-dependent phosphorylation of ATF2 plays an important role in the drug resistance phenotype likely by mediating enhanced DNA repair by a p53-independent mechanism. PMID: 12663670
  64. ATF-2 and ATF3 seem to play an important role in the protective response of human cells to ionizing radiation PMID: 12833146
  65. ATF4 and ATF2 have roles in regulating CHOP expression PMID: 14630918
  66. In melanoma strong cytoplasmic ATF2 expression was associated with primary specimens rather than metastases and with better survival. Strong nuclear ATF2 expression was associated with metastatic specimens and with poor survival. PMID: 14678960
  67. differentiation-dependent expression and phosphorylation of ATF2 protein physically and functionally interacts with C/EBPalpha and coativator ASC-2 and synergizes to induce target gene transcription during granulocytic differentiation PMID: 14734562
  68. ATF2 and HO-1 are regulated and induced by biliverdin reductase PMID: 14988408
  69. genes affected by ATF2 and ATF2-sm appear to belong to discrete groups PMID: 15691875
  70. ATF2 expression in the neuron of normal human brain. But downregulation in the Neurodegenerative Disease( Alzheimer disease, Huntington disease and Parkinson disease). PMID: 15878807
  71. Data demonstrate that the protein kinase ATM phosphorylates ATF2 on serines 490 and 498 following ionizing radiation (IR). PMID: 15916964
  72. activity of the AP-1 components c-Jun, ATF2, and c-Fos is altered in renal cystic tissue of patients with autosomal dominant polycystic kidney disease PMID: 16049073
  73. Resistin induces PTEN expression by activating stress signaling p38 pathway, which may activate target transcription factor ATF-2, which in turn induces PTEN expression PMID: 16418168
  74. These studies establish p38 MAP kinase-mediated activation of ATF-2 as a significant mechanism in amylin-evoked beta-cell death, which may serve as a target for pharmaceutical intervention and effective suppression of beta-cell failure in type-2 diabetes. PMID: 16869889
  75. Sin1 may contribute to ATF-2 signaling specificity by acting as a nuclear scaffold. PMID: 17054722
  76. Binding of ATF2 to the CD1A promoter in human monocytes suggests a role for ATF/cAMP response element binding protein family members in regulation of CD1A expression. PMID: 17082618
  77. Shear stress and KLF2 inhibit nuclear activity of ATF2, providing a potential mechanism by which endothelial cells exposed to laminar flow are protected from basal proinflammatory, atherogenic gene expression. PMID: 17244683
  78. This study demonstrates that ATF2 mediates the TGF-beta-induced MMP-2 transcriptional activation, elucidating a molecular mechanism for the malignant progression of human breast epithelial cells exerted by TGF-beta. PMID: 17258390
  79. ATF-2 may be a key regulator of the human insulin promoter possibly stimulating activity in response to extracellular signals. PMID: 17337306
  80. ATF2, but not CREB, was a target for the TAK1-JNK pathway, and p38 negatively regulated TAK1 activity through TAB1 phosphorylation. PMID: 17626013
  81. The transcription factor ATF2, which is phosphorylated and activated by JNK, is a critical mediator for inducible expression of DUSP1 and DUSP10 in this signaling pathway. PMID: 17681939
  82. ATF2/STAT3 signaling pathways are activated and may play a role in development of eccrine porocarcinoma and eccrine poroma. PMID: 17869487
  83. review of signaling pathways that activate ATF-2, as well as its downstream targets [review] PMID: 18348191
  84. analysis of regulation of ATM activation by ATF2-dependent control of TIP60 stability and activity PMID: 18397884
  85. Overexpression of phosphorylated-ATF2 and STAT3 in cutaneous squamous cell carcinoma, Bowen's disease and basal cell carcinoma. PMID: 18547788
  86. IRF2-BP1 is a JDP2-binding protein enhancing the polyubiquitination of JDP2 and represses ATF2-mediated transcriptional activation from a CRE-containing promoter. PMID: 18671972
  87. This review summarizes the current understanding of ATF2 regulation and function. PMID: 18677098
  88. overexpression of p-ATF2, p-STAT3 and possibly p53, but not p63 or p73, may contribute to the tumorigenesis of cutaneous vascular tumors. PMID: 18700251
  89. p53 and ATF-2 partly mediate the overexpression of COX-2 in H(2)O (2)-induced premature senescence of human fibroblasts. PMID: 19082758
  90. the phosphorylation of ATF-2 at Ser-121 plays a key role in the c-Jun-mediated activation of transcription that occurs in response to TPA. PMID: 19176525
  91. Data show that individuals who are homozygous for the short AP-2 beta allele display higher depression scores when psychosocial adversity is taken into account. PMID: 19184334
  92. P-ATF2 and p-STAT3 are concordantly overexpressed in extramammary Paget's disease and their expressions may possibly be associated with the tumor stage. PMID: 19278424
  93. Breast cancer patients with high ATF2 expression as detected by WB had a significantly shorter overall survival. PMID: 19331149
  94. Phospholipase D1 acts as an important regulator in house dust mite allergen Der f 2-induced expression and production of IL-13 through activation of activating transcription factor-2 activation in human bronchial epithelial cells. PMID: 19487697
  95. show that Activating transcription factor 2 induction enhances hypoxia-inducible factor 1alpha protein stability via direct protein interaction. PMID: 19712049
  96. Data reveal a new signaling pathway activated by amino acid starvation leading to ATF2 phosphorylation and subsequently positively affecting the transcription of amino acid-regulated genes. PMID: 19822663

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Subcellular Location Nucleus, Cytoplasm, Mitochondrion outer membrane
Protein Families BZIP family, ATF subfamily
Tissue Specificity Ubiquitously expressed, with more abundant expression in the brain.
Database Links

HGNC: 784

OMIM: 123811

KEGG: hsa:1386

STRING: 9606.ENSP00000264110

UniGene: Hs.592510

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