Product Details

Uniprot No.

Target Names

ATG12

Alternative Names

Apg12 antibody; APG12-like antibody; APG12L antibody; ATG12 antibody; ATG12 autophagy related 12 homolog (S. cerevisiae) antibody; ATG12 autophagy related 12 homolog antibody; ATG12_HUMAN antibody; Autophagy 12 antibody; Autophagy-related protein 12 antibody; FBR93 antibody; HAPG12 antibody; Ubiquitin-like protein ATG12 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ATG12

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The ATG12 recombinant monoclonal antibody is produced using DNA recombinant technology and in vitro genetic manipulation. The process involves immunizing an animal with a synthesized peptide derived from human ATG12, isolating B cells, and selecting positive B cells. These selected B cells undergo screening and single clone identification. The genes encoding the light and heavy chains of the ATG12 antibody are amplified via PCR and inserted into a plasmid vector to create a recombinant vector. This vector is then transfected into a host cell line for antibody expression. The ATG12 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It exhibits specificity for human ATG12 protein and finds application in ELISA.

The ATG12 protein is a key component of the autophagy pathway in cells. In the autophagy pathway, ATG12 forms a conjugate with ATG5 and ATG16L to form the ATG12-ATG5-ATG16L complex, which acts as an E3 ligase, catalyzing the lipidation of LC3. The lipidated form of LC3, called LC3-II, is recruited to the autophagosomal membrane, where it helps to form the autophagosome. The autophagosome then fuses with lysosomes, where the contents are degraded and recycled.

Target Background

Function

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.; (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established.

Gene References into Functions

miR-23a downregulation modulates the inflammatory response by targeting ATG12-mediated autophagy. PMID: 29845275
the role of the autophagy elongation complex (ATG5-12/16L1) in Hepatitis C virus replication and membranous web formation, was examined. PMID: 28067309
Ambra1 plays an important role in regulating the sensitivity of breast cancer cells to epirubicin. Regulatory effect of Ambra1 on epirubicin sensitivity is achieved through the regulation of autophagy by targeting ATG12. PMID: 30027574
The finding that miR378 targets ATG12 indicated that miR378 may have a potential role in autophagy. These findings may provide novel insights into the mechanism of metastasis in cervical cancer and a novel therapeutic target for the treatment of cervical cancer. PMID: 29488616
Results show that ATG12 is a downstream effector in MALAT1-mediated autophagy in gastric cancer cells. PMID: 29162158
HBV gained access to Atg5-12/16L1 via interaction of its core protein with the Atg12 moiety of the complex. In contrast, subsequent autophagosome maturation and closure events were unnecessary for HBV replication, as evidenced by inhibition of Atg8/LC3 conjugation. Interfering with the HBV/Atg12 cross talk may be a tool for virus control. PMID: 29367244
transcriptional activity of ATG12 gene promoter was not significantly affected by other two DNA sequence variants identified in Parkinson's disease patient PMID: 28229934
STAT3 and ATG12 are targets of miR-454-3p. PMID: 28182000
above findings suggest that ATG5-ATG12 positively regulate anti-viral NF-kappaB and IRF3 signaling during FMDV infection, thereby limiting FMDV proliferation. FMDV has evolved mechanisms to counteract the antiviral function of ATG5-ATG12, via degradation of them by viral protein 3C(pro). PMID: 28102839
rs26538 was relevant to the lower risk of coal workers' pneumoconiosis and conferred to reduce the transcription activity of ATG12's promoter. PMID: 28844669
ATG5-ATG12 is increased in hepatitis B virus-associated hepatocellular carcinoma and has a role in apoptosis PMID: 27729742
Analysis of hepatitis B virus capsid maturation steps revealed that Rab33B and Atg5/12/16L1 are required for proper particle assembly and/or stability. PMID: 25439980
reveal a novel interconnection between autophagy, proteasome activity, and cell death mediated by the ubiquitin-like properties of ATG12 PMID: 25629932
The results revealed that the expression of Atg12 and LC3 II/LC3 I in the chondrocytes treated with TXC increased, compared to that in the untreated chondrocytes PMID: 24899049
Cav-1 competitively interacts with the ATG12-ATG5 system to suppress the formation and function of the latter in lung epithelial cells. PMID: 24727585
ATG12 is a novel determinant of breast cancer primary resistance to HER1/2-targeted therapeutics. PMID: 23307622
study to identify role of conjugation between ATG12 and ATG5 in LC3 lipidation; structural and mutational analyses of ATG12~ATG5-ATG16N revealed the conjugation generates a patch across ATG12 and ATG5 required for E3 activity PMID: 23202584
These data relate LC3B, ATG5 and ATG12 to mitochondrial quality control after oxidative damage, and to cellular longevity. PMID: 22170153
The Atg12 is as a positive mediator of mitochondrial apoptosis and show that Atg12 directly regulates the apoptotic pathway by binding and inactivating prosurvival Bcl-2 family members, including Bcl-2 and Mcl-1. PMID: 22152474
Calpain1 plays an important role in controlling the levels of autophagy in normal living cells by regulating the levels of a key signaling molecule, ATG12-ATG5 conjugate. PMID: 19901552
Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p PMID: 11825910
Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing PMID: 12207896
Mitochondrial DNA deletions and chloramphenicol treatment stimulate APG12. PMID: 17457038
Knockdown of p300 reduces acetylation of Atg5, Atg7, Atg8, and Atg12, although overexpressed p300 increases the acetylation of these same proteins. PMID: 19124466

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Subcellular Location

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.

Protein Families

ATG12 family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 588

OMIM: 609608

KEGG: hsa:9140

STRING: 9606.ENSP00000425107

UniGene: Hs.264482