Code | CSB-RA152430A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The production of the BRD4 recombinant monoclonal antibody involves DNA recombinant technology and in vitro genetic manipulation. Immunizing an animal with a synthesized peptide derived from human BRD4 to isolate B cells, from which positive B cells are selected. And then positive B cell screening and single clone identification are performed. Amplifying the light and heavy chains of the BRD4 antibody through PCR and then these genes into a plasmid vector to get a recombinant vector, which is transfected into a host cell line for antibody expression. Collecting and purifying the BRD4 recombinant monoclonal antibody from the cell culture supernatant through affinity chromatography. This antibody can react with human BRD4 protein and is recommended for ELISA, WB, and IHC applications.
BRD4, a chromatin-associated protein, is a key player in regulating the expression of genes that are critical for cell cycle progression. BRD4 binds to acetylated lysine residues on histone tails, and promotes the recruitment of transcriptional machinery to specific genes, leading to the activation of gene expression. BRD4 also interacts with RNA polymerase II and is thought to help facilitate transcriptional elongation. It has been shown to be involved in regulating cell growth and differentiation. It is also implicated in a variety of diseases, including cancer and inflammation.
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