Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
| Code | CSB-EL002802HU |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human BRD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 80-93 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 83-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| The recovery of human BRD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-107 | ||||
| EDTA plasma (n=4) | 85 | 80-90 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
This Human BRD4 ELISA Kit was designed for the quantitative measurement of Human BRD4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.
There are currently no reviews for this product.
Customer need to detect the changes in BRD4 protein in mammalian cell lines treated with compound.
1. Can we check the BRD4 epitope that is targeted by the rat mAb and detection Ab, is it close to the N or C terminus?
2. Also, could we get the antibodies separately? If yes, kindly let me know the product number.
Per customers request, please provide the following information regarding Human Bromodomain-containing protein 4, BRD4 ELISA Kit CSB-EL002802HU
1) What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
2) Is the capture antibody a monoclonal or polyclonal?
3) What is the host of the capture antibody?
4) What’s the immunogen or immunogen range of the capture antibody?
5) What’s is the species of the immunogen of the capture antibody, human or something else?
6) Is the detection antibody a monoclonal or polyclonal?
7) What is the host of the detection antibody?
8) What is the immunogen or immunogen range of the detection antibody?
9) What is the species of the immunogen of the detection antibody? Human or something else?
10) What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
11) If recombinant protein, is it full length or partial?
12) What is the species of the standard– human or something else?
13) What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.
