Product Details

Uniprot No.

P14209

Target Names

CD99

Alternative Names

CD99; MIC2; MIC2X; MIC2Y; CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD antigen CD99

Species Reactivity

Human

Immunogen

A synthesized peptide

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9A5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CD99 is a heavily glycosylated transmembrane protein that plays essential roles in T-cell adhesion, leukocyte migration, and apoptosis regulation. Its distinctive expression pattern makes it a valuable marker in immunology research, particularly in studies of T-cell biology and immune cell trafficking. CD99 has also gained significant attention as a diagnostic marker, given its characteristic expression in certain hematological malignancies and small round cell tumors.

This recombinant monoclonal antibody, clone 9A5, offers the reproducibility and consistency that demanding research applications require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports reliable experimental comparisons over time. The rabbit host origin provides high affinity binding, while affinity chromatography purification ensures minimal background in sensitive detection systems.

Validation studies demonstrate robust performance across multiple experimental platforms. In western blot analysis of Jurkat whole cell lysates, this antibody detects a band at the predicted molecular weight of 28 kDa, confirming specific target recognition in this well-characterized T-cell line. Immunohistochemical staining of paraffin-embedded human pancreatic tissue shows clear signal using standard citrate buffer antigen retrieval, with recommended dilutions ranging from 1:50 to 1:500. Flow cytometry validation in fixed and permeabilized Jurkat cells reveals distinct positive staining compared to control antibody, supporting intracellular detection workflows.

The flexibility to move between western blotting, immunohistochemistry, and flow cytometry applications makes this antibody particularly useful for researchers investigating CD99 biology across different experimental contexts, from protein expression analysis to tissue localization and single-cell phenotyping studies.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.

Gene References into Functions

Besides confirming the importance of CD99, findings indicate that polymorphic variations in this gene may affect either development or progression of EWS. PMID: 27792997
CD99 is a marker of acute myeloid leukemia and myelodysplastic syndrome stem cells PMID: 28123069
CD99 expression is increased in patients with active Inflammatory bowel disease, and positively correlated with disease activity. PMID: 27866429
CD99 counteracts EWS-FLI1 in controlling NF-kappaB signaling through the miR-34a, which is increased and secreted into exosomes released by CD99-silenced Ewing sarcoma cells. PMID: 26616853
these observations suggest that CD99 is involved in the regulation of CD1a transcription and expression by increasing ATF-2. PMID: 27094031
CD99 was also paranuclear positive in 4 of 11 (36%) small cell lung carcinomas PMID: 26782152
High expression of EMMPRIN plays a crucial role in breast cancer cell proliferation, matrigel invasion and tumor formation. PMID: 27002769
Data show that CD99 antigen expression increased during in vitro differentiation of germinal center B cells and was highest in plasma cells (PCs). PMID: 26522646
Our results suggest that the CD34/CD25/CD123/CD99(+) LAIP is strictly associated with FLT3-ITD-positive cells. PMID: 25957287
The results highlight an important role of CD99 in the differentiation and activity of human osteoblasts in physiological and pathological conditions. PMID: 26000312
CD99 can suppress CD98-mediated assembly of pro-tumorigenic signaling complexes through its ability to dephosphorylate focal adhesion kinase. PMID: 26172215
CD99 expression in astrocytomas of different malignant grades might contribute to the infiltrative ability and support the importance of CD99 as a potential target to reduce infiltrative astrocytoma capacity in migration and invasion. PMID: 24797829
The dAbd C7 induced rapid and massive EWS cell death through Mdm2 degradation and p53 reactivation. Mdm2 overexpression as well as silencing of p53 in p53wt EWS cells decreased CD99-induced EWS cell death PMID: 25501132
CD99 expression in nasal lobular capillary haemangioma. PMID: 24702676
dermatofibromastrongly expresses CD99 in a diffuse pattern that may serve as evidence in distinction from dermatofibrosarcoma PMID: 24247571
CD99 induces ERK activation, increasing its membrane-bound/cytoplasmic form rather than affecting its nuclear localization. PMID: 24677094
The new antibody scFvC7 recognizes the CD99 extracellular domain. PMID: 22335486
Report purification of human antibody scFv anti-CD99 for blocking monocyte transendothelial migration. PMID: 24798881
Solid pseudopapillary neoplasm of the pancreas exhibits a very unique immunostaining pattern for CD99 that is present in all cases. PMID: 24094957
we have shown that forced expression of wild-type CD99 in osteosarcoma cells induces downregulation of genes such as ROCK2 PMID: 23644663
CXCL16, inversely correlated with CD99 expression in Hodgkin Reed-Sternberg (H/RS) cells. PMID: 23743627
It is assumed that the expression of CD99 plays an important role in the pathogenesis of psoriasis, in the processes of emigration of leukocytes, and in their tropism toward to the epidermis PMID: 24341229
we show that miR-30a-5p provides an essential link between EWS-FLI1 and CD99, two major biomarkers in Ewing sarcoma PMID: 22986530
CD99 is highly expressed in anaplastic large-cell lymphoma, and showed high rate of co-expression with ALK. PMID: 22781601
PVR resides in the recently identified lateral border recycling compartment, similar to PECAM and CD99. PMID: 23333754
We report an unusual pattern of paranuclear dot-like expression of CD99 in 14 cases of Merkel cell carcinoma, two of which did not express CK20. PMID: 23145531
CD99-induced HSP70 expression in B and T lymphocytes. PMID: 23152061
Data suggest that CD99 expression in lymph node/germinal center biopsies at time of diagnosis is not prognostic marker for survival in patients with diffuse large B-cell lymphoma treated with rituximab-CHOP immunochemotherapy. PMID: 22864685
the membrane protein CD99 was identified as a novel stromal factor with clinical relevance. The results support the concept that stromal properties have an important impact on tumour progression. PMID: 22392539
upregulation of CD99 in H/RS cells induces terminal B-cell differentiation, which may provide a novel therapeutic strategies for cHL PMID: 22020966
Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) and CD99 are critical in lymphatic transmigration of human dendritic cells. PMID: 22189791
NF-kappaB2 exhibits the major inhibitory role in the transcription at the CD99 promoter PMID: 22083306
High CD99 is associated with central primitive neuroectodermal tumors and Ewing's sarcoma PMID: 21267687
Data show that CD99 combined with E-cadherin/beta-catenin and CD10 can be used as a relatively specific expression profile of SPTs. PMID: 21775056
Our study has identified for the first time that pancreatic solid-pseudopapillary neoplasm exhibits a unique dot-like staining pattern for CD99. PMID: 21566515
CD99 positivity was significantly associated with advanced stage (p < 0.01), higher risk group according to the International Prognostic Index risk score (p < 0.01) and non-germinal center B cell-like type (p = 0.01). PMID: 21196719
Protein expression and gene promoter hypermethylation of CD99 in transitional cell carcinoma of urinary bladder PMID: 20217126
CD99 showed no preferential staining of Atypical fibroxanthoma, spindle cell malignant melanoma or sarcomatoid carcinoma/spindle cell squamous cell carcinoma PMID: 20184665
CD99 may play a physiologic role in the clonal deletion processes necessary for B-lymphoid selection PMID: 20453109
Analysis of a panel of human EWS cells revealed an inverse correlation between CD99 and H-neurofilament expression, as well as an inverse correlation between neural differentiation and oncogenic transformation. PMID: 20197622
Results suggest that type I is the major isoform of CD99 expressed in non-neoplastic gastric mucosa and gastric adenocarcinomas. PMID: 12172043
Functional involvement of src and focal adhesion kinase in a CD99 splice variant-induced motility of human breast cancer cells PMID: 12216109
engagement triggers the exocytic transport of ganglioside GM1 and the reorganization of actin cytoskeleton PMID: 12681511
inhibition by MHC class I engagement or apoptosis and upregulation of T cell receptor and MHC molecules in human thymocytes and T cell line induced by CD99 PMID: 12832073
CD99 epitopes plays a distinct role in T cell biology, especially in T cell apoptosis. PMID: 14623115
CD99 type II acts as a negative regulator in the neural differentiation of precursor cells that might occur during nerve system development PMID: 14646598
Solitary sclerotic fibroma is a fibrotic lesion with cells positive for CD34 and O13. O13 expression in SF has not been previously described. PMID: 14744088
our findings indicate that CD99 functions occur through reorganization of cytoskeleton and identify actin and zyxin as the early signaling events driven by CD99 engagement. PMID: 15184883
solution structure of cytoplasmic domain of human CD99 type I shows that it has a hairpin shape anchored by two flexible loops PMID: 15359120
cyclophilin A binds CD99 and may be either a signaling mediator or a signaling regulator for CD99 PMID: 15388255

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

CD99 family

Database Links

HGNC: 7082

OMIM: 313470

KEGG: hsa:4267

STRING: 9606.ENSP00000370588

UniGene: Hs.653349

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Code	CSB-RA004973A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in Jurkat whole cell lysate All lanes CD99 antibody at 0.8μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 KDa Observed band size: 28 KDa IHC image of CSB-RA004973A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Overlay histogram showing Jurkat cells stained with CSB-RA004973A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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CD99 Recombinant Monoclonal Antibody

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