CD99 Antibody

Datasheet

Code CSB-RA004973A0HU
Size US$350
Uniprot No. P14209
Image
  • Western Blot
    Positive WB detected in:Jurkat whole cell lysate
    All lanes:CD99 antibody at 0.8μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 28 KDa
    Observed band size: 28 KDa

  • IHC image of CSB-RA004973A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Overlay histogram showing Jurkat cells stained with CSB-RA004973A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunogen A synthesized peptide
Species Reactivity Human
Tested Applications ELISA, WB, IHC, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500
Relevance Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99, CD99, MIC2, MIC2X, MIC2Y
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Research Area Immunology
Gene Names CD99
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families CD99 family
Database Links

HGNC: 7082

OMIM: 313470

KEGG: hsa:4267

STRING: 9606.ENSP00000370588

UniGene: Hs.653349

Pathway Leukocyte transendothelial migration

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