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The generation of the TFPI recombinant monoclonal antibody follows a meticulous process to guarantee its exceptional quality and specificity. It begins by isolating B cells from an immunized animal, with the recombinant human TFPI protein used as the immunogen. Total RNA is then extracted from these B cells and converted into cDNA through reverse transcription. The TFPI antibody genes are amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is transfected into host cells, enabling the production of the TFPI recombinant monoclonal antibody. After cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography, resulting in a highly purified form ready for various applications. To ensure its reliability, ELISA is performed to validate the antibody's specificity and functionality in recognizing human and rabbit TFPI protein. This rigorous production process ensures the generation of a robust and effective TFPI recombinant monoclonal antibody, indispensable for a wide range of TFPI-related research.
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