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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%
|Three samples of known concentration were tested twenty times on one plate to assess.
|Inter-assay Precision (Precision between assays): CV%<10%
|Three samples of known concentration were tested in twenty assays to assess.
|To assess the linearity of the assay, samples were spiked with high concentrations of human TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
|The recovery of human TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
|Average % Recovery
|EDTA plasma (n=4)
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
This human TFPI ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human TFPI in serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the TFPI levels in the sample and is measured at 450 nm through a microplate reader.
TFPI is an anticoagulant protein produced primarily by the endothelium and megakaryocytes. It is the main inhibitor of the TF-dependent pathway of blood coagulation. TFPI mediates its anticoagulant activity by impeding early proteolytic events of the blood coagulation cascade, including those mediated by the TF-FVIIa catalytic complex, FXa, and early forms of the prothrombinase complex (FVa-FXa). TFPI can also enhance the effect of heparins. Studies have shown that TFPI exerts anti-angiogenic and anti-metastatic effects in vitro and in vivo. TFPI plays a significant role in the pathophysiology of many diseases, including cancer and inflammation.
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