Recombinant Human C-C chemokine receptor type 4(CCR4)

Code CSB-CF004843HU
Size US$3481
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names CCR4
Uniprot No. P51679
Research Area Others
Alternative Names C C chemokine receptor type 4; C C CKR 4; C-C chemokine receptor type 4; C-C CKR-4; CC CKR 4; CC-CKR-4; CCR 4; CCR-4; CCR4; CCR4_HUMAN; CD194; Chemokine (CC motif) receptor 4; chemokine C C motif receptor 4; ChemR13; CKR4; CMKBR 4; CMKBR4; HGCN 14099; K5 5; K5-5; MGC88293
Species Homo sapiens (Human)
Source in vitro E.coli expression system
Expression Region 1-360aa
Target Protein Sequence MNPTDIADTTLDESIYSNYYLYESIPKPCTKEGIKAFGELFLPPLYSLVFVFGLLGNSVVVLVLFKYKRLRSMTDVYLLNLAISDLLFVFSLPFWGYYAADQWVFGLGLCKMISWMYLVGFYSGIFFVMLMSIDRYLAIVHAVFSLRARTLTYGVITSLATWSVAVFASLPGFLFSTCYTERNHTYCKTKYSLNSTTWKVLSSLEINILGLVIPLGIMLFCYSMIIRTLQHCKNEKKNKAVKMIFAVVVLFLGFWTPYNIVLFLETLVELEVLQDCTFERYLDYAIQATETLAFVHCCLNPIIYFFLGEKFRKYILQLFKTCRGLFVLCQYCGLLQIYSADTPSSSYTQSTMDHDLHDAL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 44.9 kDa
Protein Length Full Length
Tag Info N-terminal 10xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
Gene References into Functions
  1. the expression of chemokine receptors in different peripheral blood T-cell subsets in patients with polymyositis (PM) and dermatomyositis, was examined. PMID: 28869080
  2. This review presents key clinical studies of Multiple sclerosis together with experimental studies in animals that have demonstrated functional roles of CCR4, CCL17, and CCL22 in experimental autoimmune encephalomyelitis pathogenesis. [review] PMID: 29099057
  3. Taken together, our data showed a likely positive feedback mechanism of enhanced IL-6 production in ectopic milieu. The high levels of IL-6 in the peritoneal cavity stimulate the expression of CCL17 in endometrial stromal cells by activating JNK signal pathway, and then, CCL17 further induces CCR4 expression on macrophages, which eventually leads to an increase in IL-6 production via NF-kappaB activation. PMID: 28240436
  4. Investigations showed that the level of CCR4 could be induced by TNF-alpha dependent of NF-kappaB activation in CRC cells. CCR4 might be implicated in TNF-alpha-regulated cancer cells metastasis. PMID: 27356745
  5. Tumor-associated macrophages promote prostate cancer migration through activation of the CCL22-CCR4 signaling axis. PMID: 28039457
  6. monocyte chemoattractant protein-1 (MCP-1), an endogenous CCR4-binding ligand, was specifically upregulated in the head and neck squamous cell carcinomamicroenvironment compared to the other four CCR4-binding ligands PMID: 27177223
  7. A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer PMID: 28134623
  8. The percentage of CXCR3(+) CD4(+) TEM cells negatively correlated with the severity of the cutaneous disease in psoriasis patients. Importantly CLA(+) CD4(+) TCM cells expressing CCR6(+) or CCR4(+)CXCR3(+) negatively correlated with psoriasis severity suggesting recruitment to the skin compartment. PMID: 28392462
  9. Case Report: CCR4+ T-cell depletion by mogamulizumab treatment of adult T-cell leukaemia/lymphoma results in CD8-positive T-cell mediated dermatitis. PMID: 27786349
  10. CCL22-specific antibodies reveal that engagement of two distinct binding domains on CCL22 is required for CCR4-mediated function. PMID: 26683175
  11. CCR4 mutation is an important determinant of the clinical course in ATLL cases, and that NS and FS mutations of CCR4 behave differently with respect to ATLL pathophysiology. PMID: 26847489
  12. Distinctive Treg associated CCR4-CCL22 expression profile with altered frequency of Th17/Treg cell in the immunopathogenesis of Pemphigus Vulgaris. PMID: 26093920
  13. Data show that CCR4 was over-expressed in lymph node-negative human gastric cancer tissues and suggest its involvement in promoting tumor invasion probably, through up-regulation of MMP9. PMID: 25790118
  14. CCR4 C1014T and CCL22 C16A genetic variations were neither associated with the risk, nor with the progression of colorectal cancer in Iranian population PMID: 25148803
  15. Higher percentages of CCR4+ CD4 TEM cells in acute RSV infection were accompanied with higher percentages of CXCR3+ CD8 TEM cells, whereas the development of long-lived memory CXCR3+ CD4 and CD8 TCM cells seems to be compromised PMID: 25013801
  16. These findings implicate somatic gain-of-function CCR4 mutations in the pathogenesis of adult T cell leukemia/lymphoma. PMID: 25488980
  17. CCR4 has a role in tumor aggressive behavior in renal cell carcinoma PMID: 24554520
  18. Data show that CCR4 exists in at least two distinct conformations, which are differentially activated by ligand. PMID: 24563252
  19. CCR4 mediates CCL17 induced cell migration via increased total RhoA and active RhoA protein levels in colon cancer cells. PMID: 23649168
  20. Anti-CCR4 monoclonal antibody treatment is instrumental for evoking and augmenting antitumor immunity in cancer patients by selectively depleting eTreg cells. PMID: 24127572
  21. Authors identify an evolutionarily conserved C-terminal motif in human TTP that directly binds a central domain of CNOT1, a core subunit of the CCR4-NOT complex. PMID: 23644599
  22. lymph node metastasis of CCR4(+) HNSCC is promoted by CCL22 in an autocrine or M2-like macrophage-dependent paracrine manner. PMID: 23180648
  23. CCR4+ memory T-cell frequency is increased in patients with Wegener's granulomatosis with polyangiitis. PMID: 22490506
  24. CCR4-, CCR5-, CXCR3-, and selectin ligand-expressing CD4+ T cells preferentially accumulate in the joints of children with juvenile idiopathic arthritis. PMID: 21739422
  25. A high amino acid charge of the envelope glycoprotein 120 V3 region is important for binding to host CXCR4 receptor. PMID: 21525208
  26. aberrant expression of CCR4 in human gastric cancer could contribute to tumor-induced immunosuppression. PMID: 21443538
  27. Lymphocyte CCR4 expression is closely associated with induction of human allergen-induced late nasal responses. PMID: 20148806
  28. high-level lineage-independent induction of CCR4 can occur following T-cell activation without accessibility-associated changes in histone H3, but that without such changes expression is transient rather than persistent. PMID: 20963786
  29. We suggest that CCR4 and CCR6 expression on CD4(+) T cells should be considered as markers of disease activity in systemic lupus erythematosus. PMID: 20334681
  30. CD4+CD25high Treg cells of Wegener granulomatosis-patients exhibited decreased numbers of cells co-expressing FoxP3 and CCR4 PMID: 20412707
  31. CCR4 might play a role in allergen-driven T helper type (Th)2 cell accumulation in asthmatic airways. PMID: 20237293
  32. CCR4 ligands secreted by dendritic cells recruit regulatory T cells (Tregs) to sites of inflammation in patients with autoimmune and chronic hepatitis. PMID: 20164417
  33. Data show that MDC/CCL22 is present in the synovial membrane of rheumatoid arthritis (RA) and psoriatic arthritis (PsA) patients and in synovial fluid of patients with RA and PsA, which would enable migration of CCR4 expressing memory cells. PMID: 19942450
  34. Data show that TH treatment promoted rapid and sustained hCCR4 recruitment to the TH-responsive deiodinase 1 promoter and TR co-localizes with hCCR4 in the nucleus and interacts with hCCR4 in 2-hybrid and pull-down assays. PMID: 19903885
  35. CCR4 is expressed with high frequency in adult T-cell leukemia and human T-cell leukemia virus type 1-transformed T cells and in ATL skin lesions. PMID: 11861261
  36. CCR4 is mainly expressed by a high cytokine (interleukin-4/interleukin-2)-producing (CD4) subset of natural killer t-cells. PMID: 12070001
  37. The skin-homing TH compartment is itself divided into distinct subpopulations, the smaller of which expresses both CCR4 and CCR10, and the larger of which expresses only CCR4. PMID: 12406880
  38. CCR4+ cells were present predominantly in the lesional skin of atopic dermatitis patients, but not in the non-lesional skin PMID: 12456591
  39. In the blood of cutaneous T cell lymphoma patients with peripheral blood involvement we found significantly increased percentages of T cells displaying the skin-homing phenotype (CLA+CCR4+) compared with healthy individuals. PMID: 12485447
  40. Increased expression of CCR4, which is proposed to guide CD25(+) Ts cells to DC, is an intrinsic feature of CD25(+) Ts cells. PMID: 12778466
  41. airway allergen-specific T(H)2 cells are CCR4(+), but in the atopic child CCR4 does not distinguish between recall antigen and allergen specificity PMID: 14657875
  42. although there is PI(3,4,5)P(3) accumulation downstream of CCR4, phosphoinositide 3-kinase activity is a dispensable signal for CCR4-stimulated chemotaxis of Th2 cells and the CEM T cell line. PMID: 15187160
  43. CXCR3 and CCR4 were heterogeneously expressed in peripheral T-cell lymphomas. PMID: 15328188
  44. CC chemokine receptor 4 has a role in adult T-Cell leukemia/lymphoma PMID: 15569983
  45. review of possible relationship of CCR4 role in T cell migration and skin infiltration in ATL, and of selective expression of CCR4 by Th2 and regulatory T cells and possible origin of ATL in Th2 or regulatory T cells. PMID: 15621800
  46. CCR4 and TARC/CCL17 play role in pathophysiology of cutaneous lupus erythematosus(CLE). Cytotoxic CD8+ T cells expressing CCR4 appear to be involved in scarring subtypes of CLE. PMID: 15955100
  47. CCR4 and CCR10 may play an important role in ATLL invasion into the skin PMID: 17071491
  48. Bexarotene reduces CCR4-positive lymphocytes. PMID: 17546636
  49. aberrantly expressed Fra-2 in association with JunD may play a major role in CCR4 expression and oncogenesis in adult T-cell leukemia. PMID: 18071306
  50. CCR4 and CCR10 are expressed on epidermal keratinocytes and that both are functional in terms of skin cytokine production and/or migration to their ligand CCL17 and CCL27, respectively. PMID: 18782672
  51. ratio of CCR4+/CXCR3+ cells was 4.45 in chemotherapy and 0.72 in immunotherapy PMID: 19106589
  52. provide further dynamic evidence, in line with the multistep cascade paradigm for leukocyte transendothelial migration, to support a critical role for CCR4 in cutaneous T-cell lymphoma migration PMID: 19239991
  53. Regulatory T cells recruited through CCL22/CCR4 are selectively activated in lymphoid infiltrates surrounding primary breast tumors and lead to an adverse clinical outcome. PMID: 19244125
  54. CCR4 was expressed on a part of the memory cell population and CCR4+CD8+ memory T cells had the ability to produce multiple cytokines including IL-4 and to migrate in response to CC chemokine receptor ligand (CCL)17/TARC and CCL22/MDC. PMID: 19261691
  55. CCR4, a chemokine receptor known to be selectively expressed by T cell subsets such as Th2 cells, skin-homing memory/effector T cells, and regulatory T cells. (review) PMID: 19374191
  56. The human CCR4 gene is localized at Chr 3p24. PMID: 8884276

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Tissue Specificity Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cell
Database Links

HGNC: 1605

OMIM: 604836

KEGG: hsa:1233

STRING: 9606.ENSP00000332659

UniGene: Hs.184926

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