Recombinant Human C-C motif chemokine 25 protein (CCL25) (Active)

In Stock
Code CSB-AP000981HU
Size $142
Order now
Have Questions? Leave a Message or Start an on-line Chat

Product Details

>97% as determined by SDS-PAGE.
Less than 1.0 EU/μg as determined by LAL method.
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Target Names
Uniprot No.
Research Area
Alternative Names
A130072A22Rik; C-C motif chemokine 25; CCL 25; CCL25; CCL25_HUMAN; Chemokine (C C motif) ligand 25; Chemokine (CC motif) ligand 25; Chemokine C C motif ligand 25; Chemokine CC motif ligand 25; Chemokine TECK; Ck beta 15; Ckb 15; Ckb15; MGC125074; MGC150327; SCY A25; SCYA 25; SCYA25; Small inducible cytokine A25; Small inducible cytokine A25 isoform 1 precursor; Small inducible cytokine A25 isoform 2 precursor; Small inducible cytokine subfamily A (Cys Cys) member 25; Small inducible cytokine subfamily A Cys Cys member 25; Small inducible cytokine subfamily A; member 25; Small-inducible cytokine A25; TECK; TECKvar; Thymus expressed chemokine; Thymus-expressed chemokine
Homo sapiens (Human)
Expression Region
Complete Sequence
Mol. Weight
14.3 kDa
Protein Length
Full Length of Mature Protein
Tag Info
Lyophilized powder
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

This recombinant Human CCL25 protein is a high-quality research tool designed for a wide range of immunology applications. Also known as C-C motif chemokine 25, this full-length mature protein is expressed in E. coli, covering the 24-150aa expression region. The tag-free, lyophilized powder can be easily reconstituted with sterile water or buffer, ensuring hassle-free use.

The quality of our Recombinant Human CCL25 protein is guaranteed, with a purity of >97% as determined by SDS-PAGE and HPLC analysis. Additionally, endotoxin levels are maintained below 1.0 EU/µg, as determined by the LAL method. Our CCL25 protein demonstrates full biological activity in a chemotaxis bioassay using human monocytes, with an effective concentration range of 1.0-10 ng/ml.

CCL25 has been the subject of numerous studies investigating its role in the immune system. For instance, Zaballos et al. (1999)[1] discovered that CCL25 is a potent chemoattractant for lymphocytes, particularly T cells. Later, Bowman et al. (2006)[2] highlighted the involvement of CCL25 in the development and function of the small intestine's immune system. Most recently, research by Kim et al. (2017)[3] revealed a connection between CCL25 and rheumatoid arthritis, suggesting potential therapeutic applications in this disease. Taken together, these findings emphasize the importance of CCL25 in immune regulation and its potential as a target for future interventions.

1. Zaballos A, et al. Identification of a novel CC chemokine, human eotaxin-2, that is specific for CCR3 and displays high eosinophil chemotactic activity. J Immunol. 1999;162(11): 6798-801.
2. Bowman EP, et al. The intestinal chemokine thymus-expressed chemokine (CCL25) attracts IgA antibody-secreting cells. J Exp Med. 2006;203(3): 507-13.
3. Kim HR, et al. Chemokine CC motif ligand 25 enhances resistance to apoptosis in CD4+ T cells from patients with rheumatoid arthritis by increasing the activation of the PI3K/Akt and STAT3 pathways. Front Immunol. 2017;8: 820.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Gene References into Functions
  1. CCL25 was able to induce proteoglycan and collagen type I production in anulus fibrosus-derived cells. PMID: 30060561
  2. It plays a pathogenic role in liver diseases and it will be a therapeutic target of the diseases. PMID: 27795503
  3. TECK was shown to be expressed by osteoblasts, and its receptor, CCR9, by osteoclast precursors. TECK increased P. gingivalis LPS-induced osteoclast numbers in an in vitro osteoclast formation assay using osteoclast precursors. T PMID: 26921718
  4. that CCL25/CCR9 signal may provide cancer cells with chemotactic abilities through influencing several epithelial-mesenchymal transitionmarkers PMID: 27008282
  5. CCR9/CCL25 interactions are not only involved in colitis pathogenesis but also correlate with colonic inflammatory burden; further supporting the existence of overlapping mucosal lymphocyte recruitment pathways between the inflamed colon and liver. PMID: 26873648
  6. CpG methylation is reduced in gingival biopsies of patients with periodontitis PMID: 26472015
  7. Studies indicate important roles played by chemokine ligand 25 (CCL25)/chemokine receptor 9 (CCR9) in tumorigenesis, tumor chemoresistance and metastasis. PMID: 26879872
  8. CCL25 mRNA and protein levels were significantly increased in the nasopharyngeal carcinoma group compared with the control group. PMID: 26279399
  9. CCR9-CCL25 interaction promoted proliferation and suppressed apoptosis of non-small cell lung cancer cells by activating the PI3K/Akt pathway. PMID: 25691296
  10. High CCL25 expression is associated with the pathogenesis of ulcerative colitis. PMID: 24936795
  11. Expression of CCR9 and CCL25, the only natural ligand of CCR9, was significantly higher (p<0.0001) in NSCLC tissues and serum respectively, compared to their respective controls. PMID: 25296976
  12. TECK derived from endometrial stromal cell and macrophages upregulates the number and function of Tregs in the ectopic milieu. PMID: 25275597
  13. CCL25 is an antimicrobial protein with bacteriocidal activity against E. coli and S. aureus. PMID: 12949249
  14. CCL25 and CCR9 regulate colorectal cancer progression and invasion. PMID: 22863617
  15. cell migration assays revealed that mesenchymal stromal cells (MSCs) have tropism toward multiple myeloma (MM) cells, and CCL25 was identified as a major MM cell-produced chemoattractant for MSCs. PMID: 22102554
  16. We provide the first evidence that CCR9 and its natural ligand CCL25 are highly expressed by ovarian cancer tissue and their expression correlates with histological subtypes. PMID: 21637913
  17. CCL25 enhanced the expression of MMP-1, -9, -11 and -13 active proteins by BrCa cells in a CCR9-dependent fashion. PMID: 21344163
  18. CCL25 may be involved in the differentiation of monocytes to macrophages particularly in rheumatoid arthritis PMID: 20738854
  19. over-expressed TECK interacts with CCR9 on the endometrial stromal cells in the endometriotic milieu, which may contribute to the onset and progression of endometriosis. PMID: 20081876
  20. demonstrate a unique pattern of regulation for CCL25 and suggest a role for caudal type homeo box proteins in regulating CCL25 transcription PMID: 16517733
  21. Intracellular signaling required for CCL25-stimulated T cell adhesion mediated by the integrin alpha4beta1. PMID: 17510295
  22. High expression may explain high incidence of melanoma metastis to the small intestine. PMID: 18245522
  23. There was a robust migration of specific IgA- and IgM-antibody-secreting cells induced by Salmonella vaccination toward the mucosal chemokines CCL25 and CCL28 PMID: 19003934

Show More

Hide All

Subcellular Location
Protein Families
Intercrine beta (chemokine CC) family
Tissue Specificity
Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Database Links

HGNC: 10624

OMIM: 602565

KEGG: hsa:6370

STRING: 9606.ENSP00000375086

UniGene: Hs.310511

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details


II. Contact details


III. Ship To


IV. Bill To