Product Details

Target Name

chemokine (C-C motif) ligand 25

Alternative Names

A130072A22Rik ELISA Kit; C-C motif chemokine 25 ELISA Kit; CCL 25 ELISA Kit; CCL25 ELISA Kit; CCL25_HUMAN ELISA Kit; Chemokine (C C motif) ligand 25 ELISA Kit; Chemokine (CC motif) ligand 25 ELISA Kit; Chemokine C C motif ligand 25 ELISA Kit; Chemokine CC motif ligand 25 ELISA Kit; Chemokine TECK ELISA Kit; Ck beta 15 ELISA Kit; Ckb 15 ELISA Kit; Ckb15 ELISA Kit; MGC125074 ELISA Kit; MGC150327 ELISA Kit; SCY A25 ELISA Kit; SCYA 25 ELISA Kit; SCYA25 ELISA Kit; Small inducible cytokine A25 ELISA Kit; Small inducible cytokine A25 isoform 1 precursor ELISA Kit; Small inducible cytokine A25 isoform 2 precursor ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 25 ELISA Kit; Small inducible cytokine subfamily A Cys Cys member 25 ELISA Kit; Small inducible cytokine subfamily A; member 25 ELISA Kit; Small-inducible cytokine A25 ELISA Kit; TECK ELISA Kit; TECKvar ELISA Kit; Thymus expressed chemokine ELISA Kit; Thymus-expressed chemokine ELISA Kit

Abbreviation

CCL25

Uniprot No.

O15444

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

25 pg/mL-1600 pg/mL

Sensitivity

6.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TECK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-98
1:2	Average %	100
	Range %	96-104
1:4	Average %	89
	Range %	84-94
1:8	Average %	95
	Range %	89-100

Recovery

The recovery of human TECK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-97
EDTA plasma (n=4)	104	97-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.684	2.564	2.624	2.498
800	2.011	1.937	1.974	1.848
400	1.374	1.339	1.357	1.231
200	0.945	0.908	0.927	0.801
100	0.528	0.516	0.522	0.396
50	0.320	0.307	0.314	0.188
25	0.238	0.231	0.235	0.109
0	0.129	0.123	0.126

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human CCL25 ELISA Kit was designed for the quantitative measurement of Human CCL25 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

Code	CSB-E09189h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human thymus expressed chemokine,TECK ELISA Kit

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