Human thymus expressed chemokine,TECK ELISA Kit

Code CSB-E09189h
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
chemokine (C-C motif) ligand 25
Alternative Names
A130072A22Rik ELISA Kit; C-C motif chemokine 25 ELISA Kit; CCL 25 ELISA Kit; CCL25 ELISA Kit; CCL25_HUMAN ELISA Kit; Chemokine (C C motif) ligand 25 ELISA Kit; Chemokine (CC motif) ligand 25 ELISA Kit; Chemokine C C motif ligand 25 ELISA Kit; Chemokine CC motif ligand 25 ELISA Kit; Chemokine TECK ELISA Kit; Ck beta 15 ELISA Kit; Ckb 15 ELISA Kit; Ckb15 ELISA Kit; MGC125074 ELISA Kit; MGC150327 ELISA Kit; SCY A25 ELISA Kit; SCYA 25 ELISA Kit; SCYA25 ELISA Kit; Small inducible cytokine A25 ELISA Kit; Small inducible cytokine A25 isoform 1 precursor ELISA Kit; Small inducible cytokine A25 isoform 2 precursor ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 25 ELISA Kit; Small inducible cytokine subfamily A Cys Cys member 25 ELISA Kit; Small inducible cytokine subfamily A; member 25 ELISA Kit; Small-inducible cytokine A25 ELISA Kit; TECK ELISA Kit; TECKvar ELISA Kit; Thymus expressed chemokine ELISA Kit; Thymus-expressed chemokine ELISA Kit
Abbreviation
CCL25
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TECK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 87-98
1:2 Average % 100
Range % 96-104
1:4 Average % 89
Range % 84-94
1:8 Average % 95
Range % 89-100
Recovery
The recovery of human TECK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 85-97
EDTA plasma (n=4) 104 97-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.684 2.564 2.624 2.498
800 2.011 1.937 1.974 1.848
400 1.374 1.339 1.357 1.231
200 0.945 0.908 0.927 0.801
100 0.528 0.516 0.522 0.396
50 0.320 0.307 0.314 0.188
25 0.238 0.231 0.235 0.109
0 0.129 0.123 0.126  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CCL25 ELISA Kit was designed for the quantitative measurement of Human CCL25 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Gene References into Functions
  1. CCL25 was able to induce proteoglycan and collagen type I production in anulus fibrosus-derived cells. PMID: 30060561
  2. It plays a pathogenic role in liver diseases and it will be a therapeutic target of the diseases. PMID: 27795503
  3. TECK was shown to be expressed by osteoblasts, and its receptor, CCR9, by osteoclast precursors. TECK increased P. gingivalis LPS-induced osteoclast numbers in an in vitro osteoclast formation assay using osteoclast precursors. T PMID: 26921718
  4. that CCL25/CCR9 signal may provide cancer cells with chemotactic abilities through influencing several epithelial-mesenchymal transitionmarkers PMID: 27008282
  5. CCR9/CCL25 interactions are not only involved in colitis pathogenesis but also correlate with colonic inflammatory burden; further supporting the existence of overlapping mucosal lymphocyte recruitment pathways between the inflamed colon and liver. PMID: 26873648
  6. CpG methylation is reduced in gingival biopsies of patients with periodontitis PMID: 26472015
  7. Studies indicate important roles played by chemokine ligand 25 (CCL25)/chemokine receptor 9 (CCR9) in tumorigenesis, tumor chemoresistance and metastasis. PMID: 26879872
  8. CCL25 mRNA and protein levels were significantly increased in the nasopharyngeal carcinoma group compared with the control group. PMID: 26279399
  9. CCR9-CCL25 interaction promoted proliferation and suppressed apoptosis of non-small cell lung cancer cells by activating the PI3K/Akt pathway. PMID: 25691296
  10. High CCL25 expression is associated with the pathogenesis of ulcerative colitis. PMID: 24936795
  11. Expression of CCR9 and CCL25, the only natural ligand of CCR9, was significantly higher (p<0.0001) in NSCLC tissues and serum respectively, compared to their respective controls. PMID: 25296976
  12. TECK derived from endometrial stromal cell and macrophages upregulates the number and function of Tregs in the ectopic milieu. PMID: 25275597
  13. CCL25 is an antimicrobial protein with bacteriocidal activity against E. coli and S. aureus. PMID: 12949249
  14. CCL25 and CCR9 regulate colorectal cancer progression and invasion. PMID: 22863617
  15. cell migration assays revealed that mesenchymal stromal cells (MSCs) have tropism toward multiple myeloma (MM) cells, and CCL25 was identified as a major MM cell-produced chemoattractant for MSCs. PMID: 22102554
  16. We provide the first evidence that CCR9 and its natural ligand CCL25 are highly expressed by ovarian cancer tissue and their expression correlates with histological subtypes. PMID: 21637913
  17. CCL25 enhanced the expression of MMP-1, -9, -11 and -13 active proteins by BrCa cells in a CCR9-dependent fashion. PMID: 21344163
  18. CCL25 may be involved in the differentiation of monocytes to macrophages particularly in rheumatoid arthritis PMID: 20738854
  19. over-expressed TECK interacts with CCR9 on the endometrial stromal cells in the endometriotic milieu, which may contribute to the onset and progression of endometriosis. PMID: 20081876
  20. demonstrate a unique pattern of regulation for CCL25 and suggest a role for caudal type homeo box proteins in regulating CCL25 transcription PMID: 16517733
  21. Intracellular signaling required for CCL25-stimulated T cell adhesion mediated by the integrin alpha4beta1. PMID: 17510295
  22. High expression may explain high incidence of melanoma metastis to the small intestine. PMID: 18245522
  23. There was a robust migration of specific IgA- and IgM-antibody-secreting cells induced by Salmonella vaccination toward the mucosal chemokines CCL25 and CCL28 PMID: 19003934

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Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Tissue Specificity
Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Database Links

HGNC: 10624

OMIM: 602565

KEGG: hsa:6370

STRING: 9606.ENSP00000375086

UniGene: Hs.310511

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