Product Details

Purity

>96% as determined by SDS-PAGE and HPLC.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR6 transfected murine BaF3 cells is in a concentration range of 0.1-10 ng/ml.

Target Names

Ccl20

Uniprot No.

O89093

Research Area

Immunology

Alternative Names

Ccl20; Larc; Scya20C-C motif chemokine 20; Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

28-97aa

Complete Sequence

ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM

Mol. Weight

8.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells. Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.

Gene References into Functions

Obesity-induced IL-6 shifts macrophage polarization towards tumor-promoting macrophages that produce the CCL-20 in the colitis-associated colorectal cancer (CAC) microenvironment. CCL-20 promotes CAC progression by recruiting CCR6-expressing B-cells and gammadelta T cells via chemotaxis. PMID: 29695802
Blocking or knockdown CCN1 expression ameliorated skin inflammation and reduced the expression of CCL20 in both imiquimod and IL-23-induced psoriasis-like mouse models. PMID: 28602508
these data indicate that CCL20/CCR6 signaling may play an important role in regulating bone mass accrual, potentially by modulating osteoblast maturation, survival, and the recruitment of osteoblast-supporting cells. PMID: 26890063
We crossed Ptf1a(Cre/+) ;Kras(G12D/+) mice with JNK1(-/-) mice to generate Ptf1a(Cre/+) ;Kras(G12D/+) ;JNK1(-/-) (Kras;JNK1(-/-) ) mice. Tumor weight was significantly lower in Kras;JNK1(-/-) mice than in Kras;JNK1(+/-) mice, whereas histopathological features were similar.we concluded that inhibition of activated JNK in pancreatic tumor stroma could be a potential therapeutic target to increase Ccl20 secretion PMID: 28837246
mutually exclusive transcriptional regulation by AP-1 (cjun/cfos) and non-canonical NF-kappaB (RelB/p52) downstream of MEK-ERK and NIK-IKK-alpha-NF-kappaB2 (p100) phosphorylation, respectively was responsible for persistent Ccl20 expression in the colonic cells. PMID: 27590109
Taken together, these data show that, in adipose tissues, IL-17A contributes to exacerbating insulin resistance-enhancing IL-6 production and promotes the infiltration of Th17 cells in cooperation with TNFalpha; these findings represent a novel hypothesis for the association between IL-17A-producing cells and type 2 diabetes. PMID: 27311858
Data (including data from studies in knockout/mutant mice) suggest that expression of Ahr (aryl-hydrocarbon receptor) and its translocation into nucleus are necessary for Ahr ligand-mediated synergistic induction of Ccl20; here, TCDD is Ahr ligand. PMID: 26259605
alpha-hemolytic streptococcus may exacerbate kidney damage in IgA nephropathy through CCL20 response to the effect of Th17 cells. PMID: 25265199
Acquired mtDNA mutations may promote tumorigenic phenotypes through up-regulation of chemokine CCL20. PMID: 25177208
Overexpression of CCL20 in human proximal tubular cells is inhibited by blockade of KCa3.1 under diabetic conditions through inhibition of the NF-kappaB pathway. PMID: 24733189
Our data indicate that estradiol is important in regulating the effects of keratinocyte growth factor on mouse uterine epithelial cell secretion of CCL20 and CXCL1. PMID: 24807244
CCL20 blockage lead to a diminished cerebral immune response in experimental pneumococcal meningitis. PMID: 24699535
This study suggests that CCL20/IL-15 can induce a strong antitumor immune response in tumor tissues and it is a suitable candidate for cancer immunotherapy. PMID: 24657179
Estrogen receptors alpha regulates CCL20/CXCL1 secretion in the female reproductive tract. PMID: 23025258
Cardiac fibroblasts could recruit Th17 cells infiltration into myocardium by secreting CCL20 in acute viral myocarditis. PMID: 24296428
Data indicate that a CCR6/CCL20 chemokine loop instructed rapid increase of B cells in the spleen in response to systemic administration of Nod1 agonists in a TNF-alpha-dependent manner. PMID: 24534531
Neutralization of CCL20 before induction of sepsis increased mortality during sepsis accompanied with increasing epithelial apoptosis in the jejunum and augmenting serum TNF-alpha. PMID: 23601903
CXCR3 promotes recruitment of Th17 cells from the blood into the liver in both human and murine liver injury. Their subsequent positioning near bile ducts is dependent on CCR6 and cholangiocyte-secreted CCL20. PMID: 22796894
Findings suggest that TNF-alpha is essential in the induction of autoimmune hepatitis (AIH) through upregulation of hepatic CCL20 expression, which allows migration of dysregulated splenic T cells. PMID: 23178752
Regulation of CCL20 expression in astrocytes is stimulated by the addition of interleukin (IL)-6, IL-6 soluble receptor, and IL-17. PMID: 22319003
Tumor-associated macrophages recruit CCR6+ regulatory T cells and promote the development of colorectal cancer via enhancing CCL20 production in mice PMID: 21559338
the healing response to corneal epithelial abrasion includes CCL20-dependent influx of CCR6(+) IL-17(+) IL-22(+) gammadelta T cells, and IL-22 contributes to the inflammatory response and promotes epithelial healing PMID: 21518851
IL-9 is produced by several T helper (Th) cell subsets in the presence of IL-4 and induces CCL-20 production by astrocytes to induce the migration of Th17 cells into the central nervous system. PMID: 21346235
Data show that the synergistic actions of pGM-CSF and pMIP3alpha presents a potentially feasible means of controlling immunogenic malignancies and provides a basis for the development of novel immunotherapeutic treatments. PMID: 20804501
CCL20 is important in mediating leukocyte recruitment early upon infection with respiratory syncytial virus. PMID: 20101616
Interfering with CCL20 significantly downregulates the expression of CD4(+)CD25(+) T cell development in mouse thymus. PMID: 18315924
through secretion of CCL20, astrocytes could play an important role in orchestrating the recruitment of specific leukocyte subsets to the inflamed CNS and in regulating CNS-targeted immune responses. PMID: 12528183
In experimental autoimmune encephalitis MIP-3 alpha is required for sensitization of T cells to antigen and for release of T cells from lymph nodes during the course of an immune response. PMID: 12794163
CCL20 plays a vital role in B-cell adhesion to the inflamed endothelium PMID: 12816871
our findings indicate that blockade of MIP-3alpha bioactivity can significantly reduce TNBS-mediated colonic injury and T cell recruitment, suggesting a role for this chemokine in the pathophysiology of intestinal inflammation. PMID: 17272517
CXCL12-CXCR4 and CCL20-CCR6 systems are involved in T lymphocyte-endothelial interaction in microvessels of the small and large intestines. PMID: 17885999
The joint cytokine milieu formed by T cells and synovial cells controls the production of CCL20 and, consequently, the recruitment of chemokine receptor 6 arthritogenic Th cells to the inflamed joints. PMID: 18025126
Our studies suggest that TLR4 expressed by tumor cells may be involved in the induction of chemokines like CCL20. PMID: 18083111
Th17 cytokines stimulate CCL20 production in vitro and in vivo, and thus provide a potential explanation of how CCR6-positive Th17 cells maintain their continual presence in psoriasis through a positive chemotactic feedback loop. PMID: 19295614
Investigated the regulation of ccl20 expression and found different NF-kappaB pathways modulate CCL20 transcription by operating on the same NF-kappaB binding site in the same cell type. PMID: 19303953
the CCR6-CCL20 axis in the choroid plexus controls immune surveillance of the CNS. PMID: 19305396

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Thymic medulla (at protein level). Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues. Expressed by IL1

Database Links

KEGG: mmu:20297

STRING: 10090.ENSMUSP00000109064

UniGene: Mm.116739