Recombinant Mouse C-X-C motif chemokine 2 protein(Cxcl2) (Active)

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Code CSB-AP001071MO
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 1.0-10 ng/ml.
Target Names Cxcl2
Uniprot No. P10889
Research Area Immunology
Alternative Names Cxcl2; Mip-2; Mip2; Scyb2C-X-C motif chemokine 2; Macrophage inflammatory protein 2; MIP2
Species Mus musculus (Mouse)
Source E.coli
Expression Region 28-100aa
Complete Sequence AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKVCLDPEAPLVQ KIIQKILNKG KAN
Mol. Weight 7.8 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
Gene References into Functions
  1. The studies revealed that, although overall structural and oligomerization features of CXCL3 and CXCL2 are similar, prominent differences were observed in their surface characteristics, thus implicating a functional divergence. PMID: 28928065
  2. TIARP independently down-regulated CXCL2 and IL-6 production by fibroblast-like synoviocytes, and the expression of chemokine receptors (CXCR1 and CXCR2) in neutrophils, with resultant reduction of neutrophil migration into arthritic joints. PMID: 27995997
  3. a paracrine role for Hippo-mediated secretion of CXCL1 and CXCL2 in the production of anti-microbial peptides (beta-defensins), iNOS, NOX2 and pro-inflammatory molecules during mycobacterial infection of the host, is reported. PMID: 27883091
  4. Taken together, the data of the present study demonstrated that TcpC can induce MIP2 production, which may contribute to the characteristic histological change associated with pyelonephritis. PMID: 28765918
  5. mip-2 siRNA and the MIP-2 antibody can reduce the inflammatory effects induced by lipopolysaccharide in macrophage cells. PMID: 28662496
  6. this study demonstrates that in vivo blocking of CXCL1 and CXCL2 can significantly reduce the Mycobacterium tuberculosis-induced bioactive IL-1beta production PMID: 28739876
  7. These data identify suppression of CXCL2 and CXCL5 chemoattractant expression by 11beta-HSD1 as a novel mechanism with potential for regulation of neutrophil recruitment to the injured myocardium, and cardiac fibroblasts as a key site for intracellular glucocorticoid regeneration during acute inflammation following myocardial injury. PMID: 28522730
  8. findings show that SRC-3 contributes to host defense against enteric bacteria, at least in part via upregulating CXCL2 expression to recruit neutrophils PMID: 28053238
  9. p53-mediated induction of PAI-1 expression due to chronic CS exposure exacerbates lung inflammation through elaboration of CXCL1, CXCL2, and CXCR2. PMID: 26747783
  10. Social defeat induced an exposure-dependent increase in mRNA levels of E-selectin, CXCL1, and CXCL2 that increased with additional days of social defeat. PMID: 25445193
  11. MIP-2 directly impairs neonatal pulmonary endothelial cell migration in vitro. PMID: 26163511
  12. SIRT2 regulates LPS induced proximal renal tubules CXCL2 protein expression. PMID: 25349202
  13. miR26a/-26b-COX-2-MIP-2 loop regulates allergic inflammation and the feedback relationship between allergic inflammation and the enhanced tumorigenic and metastatic potential PMID: 25907560
  14. huH1N1 virus PA and NA mediated increased MIP-2 expression early postinfection, resulting in substantial pulmonary neutrophilia with enhanced lung pathology and disease PMID: 25762737
  15. Syndecan-1 is expressed in the parietal peritoneum microvasculature but does not regulate leukocyte recruitment and is not necessary for the presentation of the chemokine MIP-2 in this tissue. PMID: 25184228
  16. Identified TLR2 and S100A8/S100A9 as key regulators of hepatic CXCL-2 expression and neutrophil recruitment. PMID: 24333183
  17. Suggest anti-inflammatory action of daidzein in lung epithelial cells is mediated via a direct interaction with PARP-1, which inhibits RelA/p65 protein PARylation required for the transcriptional modulation of pro-inflammatory chemokines such as Cxcl2. PMID: 24632845
  18. suppression of CXCL2 expression may contribute to the beneficial effect of Am80 as a therapeutic agent for intracerebral hemorrhage. PMID: 24659080
  19. Neutrophil recruitment and the neutrophil cytokines, CXCL1/CXCL2, were suppressed in apo(a)transgenic mice in the abdominal aortic aneurysm model. PMID: 24650562
  20. These data identify a cytokine circuit that involves IL-1beta-induced production of CXCL1 and CXCL2 and leads the recruitment of neutrophils to streptococcal infection sites. PMID: 25114117
  21. Data indicate that levels of toll-like receptors TLR4/4-stimulated chemokines CXCL1 and CXCL2 were selectively enhanced in stressed macrophages via receptor-interacting protein kinase 1 (RIPK1). PMID: 24920846
  22. Commensal bacteria-dependent select expression of CXCL2 contributes to periodontal tissue homeostasis. PMID: 23433011
  23. these results strongly suggest that both lack of HOCl and accumulation of H2O2 due to MPO deficiency contribute to the up-regulation of MIP-2 production in mouse neutrophils stimulated with zymosan PMID: 23438680
  24. Interferonalpha stimulates production of maladaptive proinflammatory CXCL2 by renal tubular cells. PMID: 23657854
  25. the model that mast cells, optimally positioned in close proximity to the vasculature, initiate an early phase of neutrophil recruitment by releasing the chemoattractants CXCL1/CXCL2. PMID: 23645836
  26. The results of this study suggested that MIP-2gamma mediates the pathogenesis of central nervous system disorders associated with neutrophil infiltration in the brain and decreased GLT-1 activity. PMID: 23234294
  27. Systemic treatment with CXCL12 promotes a more stable atherosclerotic lesion phenotype and enhances the accumulation of smooth muscle progenitor cells in these lesions without promoting atherosclerosis. PMID: 23393393
  28. these results suggest that increased production of reactive oxygen species by ATP-stimulated macrophages activates the signalling pathways that promote MIP-2 production which, in turn, induces neutrophil migration PMID: 22564028
  29. TNF-alfa and CXCL-2 mRNAs is induced in mice infected with Leptospira. PMID: 22342618
  30. results show that augmentation of the MIP-2/CXCR2 axis by hyperacetylation of histone H3 on the promoter region of MIP-2 and CXCR2 located in the injured peripheral nerve elicits chronic neuroinflammation through neutrophil accumulation PMID: 22135382
  31. Lipopolysaccharide stimulates the induction of CXCL2 in bone marrow macrophages (BMMs), osteoclast precursors, at the transcription level. PMID: 21507677
  32. MIP-2/CXCL2 induced by Chlamydia pneumoniae infection may initiate lymphocytic lung infiltration that can have long-term effects on tissue (ectopic lymphoid tissue formation) after clearance of active infection. PMID: 20840653
  33. Rho-kinase signalling regulates TNF-alpha and CXC chemokine formation as well as lipid peroxidation in the reperfused colon. PMID: 20593289
  34. The aim of this study was to determine whether acinar cells are a source of KC and MIP-2 and to understand their transcriptional regulation. PMID: 20671197
  35. ATP increases chemokine CXCL2 production via both NFAT transcription factor and protein kinase C/MAP kinase signaling pathways by purinergic receptor P2X7 stimulation in microglia. PMID: 20477948
  36. Receptor activator of NF-kappa B ligand-induced CXCL2 in osteoclast (OC) precursors plays a key role in the attachment, migration, differentiation, and function of OCs. PMID: 20357249
  37. Data show that rotavirus-infected macrophages promote neutrophil chemotaxis in response to rhesus rotavirus type A. PMID: 20234283
  38. The distribution of the functional IL-8 homologues CXCL1/KC, CXCL2/MIP-2, and CXCL5-6/LIX in resting and inflamed murine vessels, is examined. PMID: 20007247
  39. Neutralization of inflammatory chemokine MIP-2 (CXCL2/GRO-beta) attenuates the inflammation, weight loss, and clinical presentation of heterologously infected mice without impacting bacterial burden. PMID: 20065113
  40. These data give strong evidence that TRPC6 operates downstream to CXC-type Gq-protein-coupled chemokine receptors upon stimulation with MIP-2 and is crucial for the arrangement of filamentous actin in migrating neutrophils. PMID: 18983454
  41. Migration of PMNs to the CNS coincided with increased expression of transcripts specific for the CXCR2 ELR-positive chemokine ligands CXCL1, CXCL2, and CXCL5 within the brain PMID: 19893623
  42. Neutrophil-directed MIP-2 expression and protein secretion are low at the incisional site during the first 6 hrs after experimental surgical injury and are at a high level 24 hrs following injury. PMID: 11907123
  43. in study of relevance of chemokine expression to selective migration of t-cells and the disease localization in murine graft-versus-host disease, Mip2 was found to be predominantly expressed in spleen, liver, and skin, and not heart. PMID: 12098066
  44. Macrophage inflammatory protein-2 regulates corneal neovascularization induced by infection with Pseudomonas aeruginosa PMID: 12121220
  45. NO generated after administration of E. coli serves as an important proinflammatory signal to up-regulate MIP-2 expression in vivo. PMID: 12165537
  46. Plasma levelof thisprotein was decreased significantly by clodronic acid-encapsulating liposomes in sepsis induced by P.aeruginosa. PMID: 12604029
  47. High tidal volume ventilation in absence of underlying injury induces intrapulmonary TNF-alpha and MIP-2 expression in mice. PMID: 12807894
  48. These data show that macrophage inflammatory protein (MIP-2) is important for hepatocyte proliferation after partial hepatectomy and that pharmacological MIP-2 doses after hepatic injury accelerate hepatic regeneration. PMID: 12875976
  49. induction of secretion in fibrosarcoma cells by NFkappaB and activation of lymphotoxin beta receptor PMID: 12957791
  50. CXC chemokines play a fundamental role in colonic ischemia/reperfusion and that functional interference with CXC chemokines may protect against pathological inflammation in the colon PMID: 14583342

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Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

KEGG: mmu:20310

STRING: 10090.ENSMUSP00000074885

UniGene: Mm.4979

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