Recombinant Mouse Granulocyte colony-stimulating factor protein (Csf3) is expressed in E. coli and contains the full length of the mature protein spanning amino acids 31 to 208. This product comes without tags and achieves a purity level greater than 98%, as confirmed through SDS-PAGE analysis. The protein shows full biological activity, with an ED50 of less than 0.05 ng/ml in cell proliferation assays. Endotoxin levels are kept strictly below 1.0 EU/µg, which helps ensure high-quality research applications.
Granulocyte colony-stimulating factor (Csf3) appears to be crucial for regulating granulocyte production and function. It likely plays a significant role in hematopoiesis by stimulating the proliferation and differentiation of precursor cells into mature granulocytes. Csf3 seems integral to immune response modulation and is widely studied in contexts such as bone marrow recovery and inflammatory processes. This makes it an essential protein in research focused on immunology and hematological studies.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Cell Proliferation and Viability Assays for Hematopoietic Research
This recombinant mouse Csf3 protein can stimulate proliferation of granulocyte precursor cells in controlled in vitro experiments. The confirmed biological activity with an ED50 of less than 0.05 ng/ml using murine NFS-60 cells makes it suitable for dose-response studies and cell culture optimization. Researchers can work with this protein to investigate granulopoiesis mechanisms, test how other compounds affect granulocyte development, or maintain specific cell lines that require Csf3 for growth. The high purity (>98%) and low endotoxin levels may help ensure reliable and reproducible experimental results.
2. Cytokine Signaling Pathway Studies
The biologically active recombinant Csf3 protein serves as a valuable tool for investigating cytokine receptor binding and downstream signaling cascades in mouse cell systems. Researchers can work with this protein to study Csf3 receptor activation, phosphorylation events, and transcriptional responses in various cell types. Since the protein lacks fusion tags, signaling studies should reflect native protein interactions without potential interference from added sequences. This application appears particularly relevant for understanding how Csf3 regulates cellular responses at the molecular level.
3. Antibody Development and Validation
This high-purity recombinant mouse Csf3 protein can serve as an antigen for generating specific antibodies or as a standard for validating existing anti-Csf3 antibodies. The mature protein sequence (31-208aa) represents the biologically relevant form, making it potentially ideal for producing antibodies that recognize the native protein. Researchers can work with this protein in ELISA development, Western blot validation, or immunoassay standardization. The confirmed biological activity also allows for functional antibody testing to determine whether generated antibodies can neutralize or enhance Csf3 activity.
4. Protein-Protein Interaction Studies
The recombinant Csf3 protein can be used in biochemical assays to identify and characterize protein interactions relevant to granulocyte development and function. Researchers can incorporate this protein in pull-down experiments, surface plasmon resonance studies, or other binding assays to investigate interactions with receptors, binding proteins, or other regulatory molecules. High purity and biological activity suggest that observed interactions may reflect physiologically relevant binding events. Such studies could provide insights into the molecular mechanisms underlying Csf3 function and regulation.
5. Comparative Species and Evolutionary Studies
This mouse-specific recombinant Csf3 protein enables comparative studies with human or other species' Csf3 proteins to understand evolutionary conservation and species-specific differences. Researchers can compare biological activities, receptor binding affinities, or structural properties across species using standardized assay conditions. The well-characterized activity profile provides a reliable reference point for such comparative analyses. These studies may inform translational research efforts and help identify species-specific considerations for experimental design.
