Recombinant Mouse Granulocyte colony-stimulating factor(Csf3)

Code CSB-YP006049MO
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Source Yeast
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Code CSB-EP006049MO
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Source E.coli
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Code CSB-EP006049MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006049MO
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Source Baculovirus
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Code CSB-MP006049MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Csf3
Uniprot No. P09920
Alternative Names Csf3; CsfgGranulocyte colony-stimulating factor; G-CSF
Species Mus musculus (Mouse)
Expression Region 31-208
Target Protein Sequence VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Gene References into Functions
  1. We confirmed that acute lung injury was associated with high serum G-CSF levels, and elevated neutrophil elastase activity in the lungs and serum of mice with adenine-induced acute kidney injury (AKI). G-CSF could be a target for new anti-lung injury strategy in patients with AKI. PMID: 30056257
  2. The opposing roles of G-CSF and IFNgamma in regulation of innate inflammatory responses in a murine viral encephalitis model reveal G-CSF as a potential therapeutic target. PMID: 29352287
  3. central regulator of the transition to postinflammatory chronic visceral pain PMID: 28973941
  4. We propose that in aggressive pancreatic ductal adenocarcinoma , elevated G-CSF contributes to tumor progression through promoting increases in infiltration of neutrophil-like cells with high immunosuppressive activity. Such a mechanism provides an avenue for a neoadjuvant therapeutic approach for this devastating disease PMID: 28775207
  5. these data provide strong evidence for a role for G-CSF in the development of ACI after burn injury through suppression of EPO signaling in bone marrow erythroid cells. PMID: 28867537
  6. physiologically produced G-CSF not only acts as a neutrophil mobilizer at the relatively late stage of acute inflammation, but also prevents exaggerated neutrophil mobilization. PMID: 27551153
  7. data demonstrate that G-CSF is a pivotal driver of the disease progression in the K/BxN serum-transfer arthritis (STA) model and possibly acts in part by regulating neutrophil numbers in the circulation PMID: 26848119
  8. SB203580 increases G-CSF expression in macrophages by increasing the stability of G-CSF mRNA via its 3'UTR, and the effect was not due to its inhibition of p38 MAPK activity. PMID: 26772539
  9. Results suggested that G-CSF plays an important role in preventing colitis, likely through populating immune regulatory macrophages in the intestine. PMID: 26687628
  10. findings provide convincing evidence that monophosphoryl lipid A-induced G-CSF facilitates early expansion, mobilization, and recruitment of neutrophils to the site of infection after burn injury PMID: 26538529
  11. Overexpression of VEGF may compensate for the G-CSF deficit through preservation of cellular components, including blood vessels, in the postinfarction heart. PMID: 25976246
  12. rapidly induces autophagy after spinal cord injury to inhibit neuronal apoptosis PMID: 26524416
  13. G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy. PMID: 25865621
  14. constitutive activation of the NF-kappaB pathway in CAIX-depleted cells restored G-CSF secretion PMID: 25623234
  15. Exposure of Sca-1(+) cells to G-CSF in the culture medium for 72 h induced time-dependent but self-limiting cell cycle acceleration with a restricted effect on the CSC proliferation. PMID: 25160782
  16. these results suggest that the G-CSF pathway regulates the production of autoantibodies in murine models of lupus. PMID: 23566364
  17. G-CSF acts in a cell intrinsic manner to expand multipotent progenitors to increase production of tumor-derived Ly6G+ neutrophils. PMID: 25624500
  18. Endothelial cell(EC)-intrinsic MYD88 signaling and subsequent G-CSF production by ECs is required for myeloid progenitor lineage skewing toward granulocyte-macrophage progenitors, increased colony-forming unit granulocyte activity. PMID: 24990886
  19. Adventitial CXCL1/granulocyte-colony stimulating factor expression in response to aortic dissection triggers local neutrophil recruitment and activation. This leads to adventitial inflammation via IL-6 and results in aortic expansion and rupture. PMID: 25563839
  20. Cultured mouse enteric nervous system -neurospheres show increased expansion with increased G-CSF concentrations, in contrast to central nervous system - derived spheres. PMID: 24253464
  21. G-CSF STAT3 axis constitutes a key protective mechanism induced by injury to reduce the risk for posttraumatic infection. PMID: 24470495
  22. After deletion of Pten in mice lacking G-CSF, the splenomegaly, myeloproliferative disease, and splenic hematopoietic stem cell accumulation are rescued. PMID: 24127490
  23. Our results suggest 5-AED survival enhancement is G-CSF-dependent, and that it stimulates innate immune cell function and reduces radiation-induced DNA damage via induction of genes that modulate cell cycle progression and apoptosis. PMID: 22843381
  24. activation of the RAS/MEK/ERK pathway regulates G-CSF expression through the Ets transcription factor PMID: 23530240
  25. G-CSF protein is necessary and sufficient to restore monocyte chemoattractant protein (MCP)-1 deficiency in neutrophil-mediated host immunity following Klebsiella pneumoniae lung infection. PMID: 23129755
  26. Ang2 deficiency results in enhanced recruitment of myeloid cells depending on G-CSF, and that the enhancement results in more aggressive tumor growth and neo-angiogenesis during liver colonization. PMID: 22699974
  27. Il-1rn knockout mice exhibit delayed resolution in acute lung inflammation after exposure to lipopolysaccharide, a process that appears to be mediated via the granulocyte colony-stimulating factor/IL-17A axis. PMID: 22592923
  28. Induction of Bv8 expression by granulocyte colony-stimulating factor in CD11b+Gr1+ cells: key role of Stat3 signaling. PMID: 22528488
  29. BMMCs and G-CSF co-administration exhibits synergistic beneficial effect over time. PMID: 21699735
  30. granulocyte and macrophage populations of murine bone marrow cells are regulated by G-CSF and CD137 protein PMID: 21179444
  31. TLR2 signaling in G-CSF mobilized PBSCs correlates with their ability to rapidly differentiate into myeloid cells, resulting in improved engraftment PMID: 21239180
  32. G-CSF is crucial for skeletal myocyte development and regeneration PMID: 21422169
  33. G-CSF and IL-6 provide signals that determine the angiogenic potential of BM resident monocytes. PMID: 20354107
  34. G-CSF stimulates the expression of the MIP-2 receptor via STAT3-dependent transcriptional activation of Il8rb PMID: 20185584
  35. G-CSF exerts potent anti-apoptotic activities towards motoneurons in vivo and suggests that the protection offered by G-CSF in ALS mouse models is due to its direct neuroprotective activity PMID: 20178614
  36. Overexpression of STAT3beta did not alter the kinetics of G-CSF-mediated neutrophilic differentiation or p27 induction in 32D/G-CSF-R WT cells. PMID: 11920194
  37. Increased production of G-CSF in mice mounting the acute phase response is a key physiological component of host defense. PMID: 12097396
  38. Evaluation of role of G-CSF in the production, survival, and release of neutrophils from bone marrow into circulation using G-CSF-deficient mice PMID: 12130495
  39. G-CSF induces neutrophil mobilization indirectly through the generation of trans-acting signals PMID: 12387736
  40. G-CSF mediates granulopoiesis and, as a corollary, participates in neutrophilia in LFA-1 deficient mice. PMID: 12734371
  41. regulation of G-CSF levels may provide a mechanism for directing primitive hematopoietic progenitors into the common myeloid lineage in response to environmental stresses PMID: 12893769
  42. G-CSF treatment leads to Lyn-mediated tyrosine phosphorylation of Gab2, which may serve as an important intermediate of enhanced Akt activity and myeloid differentiation, not growth/survival response. PMID: 14656892
  43. G-CSF signaling in neutrophils is negatively regulated by SOCS3 PMID: 14699146
  44. observations show that lipocalin 24p3 is not involved in the granulocyte-colony stimulating factor withdrawal-induced apoptosis PMID: 14703690
  45. critical role for G-CSF in driving joint inflammation and as a potential therapeutic target in inflammatory joint diseases, such as rheumatoid arthritis. PMID: 15272075
  46. G-CSF induces stabilization of Fli-1 protein in myeloid cells PMID: 15557108
  47. G-CSF treatment significantly improved survival and liver histology in chemically injured mice, predominantly by promoting endogenous repair mechanisms; immunohistochemistry showed a higher percentage of bone marrow-origin hepatocytes PMID: 15661404
  48. Febrile-range hyperthermia-induced expression of G-CSF drives the sustained peripheral neutrophilia that occurs during sustained (36 h) hyperthermia in a conscious temperature-clamped mouse model. PMID: 15829718
  49. G-CSF modulates angiogenesis by increasing myelomonocytic cells (VEGFR1+ neutrophils) and their release of VEGF PMID: 16223785
  50. monocytes mobilized into the blood by G-CSF or AMD3100 stimulate angiogenesis at sites of ischemia through a paracrine mechanism PMID: 16735597

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Subcellular Location Secreted.
Protein Families IL-6 superfamily
Database Links

KEGG: mmu:12985

STRING: 10090.ENSMUSP00000037762

UniGene: Mm.1238

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