Canine Interferon alpha-1/2 ELISA kit

Product Details

Customer Reviews and Q&A

Target Background

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Canine Interferon alpha-1/2 ELISA kit

Protocol MSDS

Code	CSB-E09849c
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Customer Reviews and Q&A
Target Background

Target Name

Interferon alpha-1/2

Alternative Names

Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit

Abbreviation

IFN-A1/2

Uniprot No.

P81255

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.12 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-99
1:2	Average %	100
	Range %	94-109
1:4	Average %	92
	Range %	89-96
1:8	Average %	87
	Range %	83-96

Recovery

The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	94	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.815	2.824	2.820	2.726
400	2.417	2.425	2.421	2.327
200	1.834	1.863	1.849	1.755
100	1.042	1.112	1.077	0.983
50	0.518	0.563	0.541	0.447
25	0.248	0.269	0.259	0.165
12.5	0.126	0.131	0.129	0.035
0	0.091	0.096	0.094

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Dog IFN-A1/2 ELISA Kit was designed for the quantitative measurement of Dog IFN-A1/2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

Subcellular Location

Secreted.

Protein Families

Alpha/beta interferon family

Database Links

KEGG: cfa:100685213

UniGene: Cfa.4550

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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