Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit

Code CSB-EL002359HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9)
Alternative Names
ATP5MC1; ATP5G1; ATP synthase F(0 complex subunit C1, mitochondrial; ATP synthase lipid-binding protein; ATP synthase membrane subunit c locus 1; ATP synthase proteolipid P1; ATP synthase proton-transporting mitochondrial F(0 complex subunit C1; ATPase protein 9; ATPase subunit c
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
23.5 pg/mL-1500 pg/mL
5.86 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5G1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %89
Range %86-92
1:2Average %94
Range %89-98
1:4Average %89
Range %85-93
1:8Average %95
Range %91-99
The recovery of human ATP5G1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-97
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
15002.637 2.612 2.625 2.480
7502.215 2.118 2.167 2.022
3751.697 1.683 1.690 1.545
187.51.107 1.118 1.113 0.968
940.611 0.621 0.616 0.471
470.365 0.372 0.369 0.224
23.50.242 0.231 0.237 0.092
00.146 0.144 0.145  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Human ATP5G1 ELISA Kit was designed for the quantitative measurement of Human ATP5G1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL .

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Target Background

(From Uniprot)
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
Gene References into Functions
  1. ATP5G1, ATP5G2, and ATP5G3 of the ATP synthase are not involved in forming the permeability transition pore. PMID: 28289229
  2. ATP5G1 turnover increases upon depletion of ZC3H14, double knockdown of ZC3H14 and the nonsense-mediated decay factor, UPF1, rescues ATP5G1 transcript levels. Furthermore, fractionation reveals an increase in the amount of ATP5G1 pre-mRNA that reaches the cytoplasm when ZC3H14 is depleted and that ZC3H14 binds to ATP5G1 pre-mRNA in the nucleus. PMID: 27563065
  3. The subunit c isoforms are nonredundant, because they differ functionally by their targeting peptides, which, in addition to mediating mitochondrial protein import, play a yet undiscovered role in respiratory chain maintenance. PMID: 19889836
Subcellular Location
Mitochondrion membrane; Multi-pass membrane protein.
Protein Families
ATPase C chain family
Database Links

HGNC: 841

OMIM: 603192

KEGG: hsa:516

STRING: 9606.ENSP00000348205

UniGene: Hs.80986

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