Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit

Instructions
Code CSB-EL002359HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9)
Alternative Names AT5G1_HUMAN ELISA Kit; ATP synthase c subunit ELISA Kit; ATP synthase F(0) complex subunit C1; mitochondrial ELISA Kit; ATP synthase F(0) complex subunit C2; mitochondrial ELISA Kit; ATP synthase F(0) complex subunit C3; mitochondrial ELISA Kit; ATP synthase lipid binding protein mitochondrial ELISA Kit; ATP synthase lipid-binding protein ELISA Kit; ATP synthase proteolipid P1 ELISA Kit; ATP synthase proteolipid P2 ELISA Kit; ATP synthase proteolipid P3 ELISA Kit; ATP synthase proton-transporting mitochondrial F(0) complex subunit C1 ELISA Kit; ATP synthase proton-transporting mitochondrial F(0) complex subunit C2 ELISA Kit; ATP synthase proton-transporting mitochondrial F(0) complex subunit C3 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c (subunit 9); isoform 1 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c (subunit 9); isoform 2 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c; isoform 1 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c; isoform 2 ELISA Kit; ATP synthase; H+ transporting; mitochondrial F0 complex; subunit c; isoform 3 ELISA Kit; ATP synthase; H+ transporting; mitochondrial Fo complex; subunit C1 (subunit 9) ELISA Kit; ATP synthase; H+ transporting; mitochondrial Fo complex; subunit C2 (subunit 9) ELISA Kit; ATP synthase; H+ transporting; mitochondrial Fo complex; subunit C3 (subunit 9) ELISA Kit; ATP synthase; mitochondrial; C subunit-3 ELISA Kit; ATP5A ELISA Kit; ATP5G ELISA Kit; ATP5G1 ELISA Kit; ATPase protein 9 ELISA Kit; ATPase subunit 9 ELISA Kit; ATPase subunit c ELISA Kit; mitochondrial ELISA Kit; mitochondrial ATP synthase; subunit 9; isoform 1 ELISA Kit; mitochondrial ATP synthase; subunit 9; isoform 2 ELISA Kit; mitochondrial ATP synthase; subunit 9; isoform 3 ELISA Kit; mitochondrial ATP synthase; subunit C (subunit 9); isoform 2 ELISA Kit; mitochondrial ATP synthase; subunit C; isoform 1 ELISA Kit; mitochondrial ATP synthase; subunit C; isoform 2 ELISA Kit; mitochondrial ATP synthase; subunit C; isoform 3 ELISA Kit; P3 ELISA Kit
Abbreviation ATP5G1
Uniprot No. P05496
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5G1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %86-92
1:2Average %94
Range %89-98
1:4Average %89
Range %85-93
1:8Average %95
Range %91-99
Recovery
The recovery of human ATP5G1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-97
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.637 2.612 2.625 2.480
7502.215 2.118 2.167 2.022
3751.697 1.683 1.690 1.545
187.51.107 1.118 1.113 0.968
940.611 0.621 0.616 0.471
470.365 0.372 0.369 0.224
23.50.242 0.231 0.237 0.092
00.146 0.144 0.145  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
Gene References into Functions
  1. ATP5G1, ATP5G2, and ATP5G3 of the ATP synthase are not involved in forming the permeability transition pore. PMID: 28289229
  2. ATP5G1 turnover increases upon depletion of ZC3H14, double knockdown of ZC3H14 and the nonsense-mediated decay factor, UPF1, rescues ATP5G1 transcript levels. Furthermore, fractionation reveals an increase in the amount of ATP5G1 pre-mRNA that reaches the cytoplasm when ZC3H14 is depleted and that ZC3H14 binds to ATP5G1 pre-mRNA in the nucleus. PMID: 27563065
  3. The subunit c isoforms are nonredundant, because they differ functionally by their targeting peptides, which, in addition to mediating mitochondrial protein import, play a yet undiscovered role in respiratory chain maintenance. PMID: 19889836
Subcellular Location Mitochondrion membrane, Multi-pass membrane protein
Protein Families ATPase C chain family
Database Links

HGNC: 841

OMIM: 603192

KEGG: hsa:516

STRING: 9606.ENSP00000348205

UniGene: Hs.80986

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