Human Aquaporin 3,AQP-3 ELISA Kit

Instructions
Code CSB-E08251h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name aquaporin 3 (Gill blood group)
Alternative Names AQP 3 ELISA Kit; AQP-3 ELISA Kit; Aqp3 ELISA Kit; AQP3_HUMAN ELISA Kit; Aquaglyceroporin-3 ELISA Kit; Aquaporin 3 (GIL blood group) ELISA Kit; Aquaporin 3 (Gill blood group) ELISA Kit; Aquaporin-3 ELISA Kit; Aquaporin3 ELISA Kit; GIL ELISA Kit; Gill blood group ELISA Kit
Abbreviation AQP3
Uniprot No. Q92482
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %93
Range %90-98
1:10Average %90
Range %85-100
1:20Average %95
Range %89-99
1:40Average %90
Range %82-98
Recovery
The recovery of human AQP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9789-105
EDTA plasma (n=4)9590-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.123 2.131 2.127 1.994
101.774 1.792 1.783 1.650
51.156 1.163 1.160 1.027
2.50.542 0.549 0.546 0.413
1.250.351 0.348 0.350 0.217
0.6250.230 0.241 0.236 0.103
0.3120.179 0.183 0.181 0.048
00.132 0.134 0.133  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).
Gene References into Functions
  1. the present data suggest that expression of AQP3 and 5 may be a marker for distinguishing between patients with dry mouth and those with Sjogren's syndrome PMID: 29925705
  2. Expression of AQP3 was markedly upregulated and miR-488 was significantly upregulated in osteosarcoma (OS) tissues and cell lines. miR488 inhibited the proliferation, invasion and epithelial-mesenchymal transition (EMT) of OS cells by directly downregulating AQP3 expression, and miR488 targeting AQP3 was responsible for inhibition of the proliferation, invasion and EMT of OS cells. PMID: 30015825
  3. TFF peptides promptly produced at sites of injury increase AQP levels, most notably AQP3, thereby enhancing the cells' ability to rapidly change their shape as part of the restitutive process. TFF peptides also require functioning AQP3 channels to induce cell migration. PMID: 29046361
  4. an altered expression of placental AQP3 may produce failures in placentation such as in preeclampsia. PMID: 29567477
  5. Data show that AQP3 is localized largely in the cytoplasm and can be targeted to the plasma membrane in prostate cancer cells via a cAMP/PKA/RalA pathway. PMID: 29532894
  6. placental expression reduced in pre-eclampsia PMID: 29108637
  7. In response to injury, AQP3-depleted colonic epithelial cells showed defective lamellipodia, focal adhesions, and repair after wounding, along with impaired H2O2 responses after exposure to the intestinal pathogen Citrobacter rodentium. PMID: 28049834
  8. AQP3 siRNA and PLD2 siRNA significantly downregulated the mRNA and protein levels of AQP3 and PLD2 in the A431 cells; inhibiting proliferation and promoting apoptosis in vitro. PMID: 28656282
  9. Reduced epidermal AQP-3 may have a role in impaired melanocyte survival in vitiligo, and might be a potential negative biological marker for vitiligo activity PMID: 26923934
  10. provide evidence for the involvement of the JNK pathway in AQP3 regulation in isolated oligohydramnios tissues in vitro, in amniotic epithelial cells derived from normal amniotic fluid and fluid from patients with isolated oligohydramnios PMID: 27814615
  11. Results indicate that AQP3 promotes stem-like properties of gastric carcinoma (GC) cells by activating the Wnt/GSK-3beta/beta-catenin signaling pathway. PMID: 26918728
  12. The Adiponectin increases AQP3 expression through PPARalpha-mediated signaling inhepatic stellate cells. PMID: 28595905
  13. skin dryness observed in intrinsic and extrinsic aged skin may be explained, at least in part, by AQP3 downregulation. This may open new avenues sufficient to control skin texture and beauty. Its interaction in skin protein organization and gene polymorphism can also be tackled in future research PMID: 26509906
  14. epigenetic signatures at AQP3 and SOCS3 engage in low-grade inflammation across different tissues, possible via JAK/STAT mediated pathways PMID: 27824951
  15. RNA interference (RNAi) of aquaporin 3 (AQP3) retarded the growth and invasiveness of XWLC-05 lung cancer cells and decreased the activity of matrix metalloproteinase 2 (MMP2). PMID: 28189680
  16. Taken together, our data, as a proof of principle, suggest that AQP3 can promote tumor growth of pancreatic cancer cells by activating the Mtor signaling pathway and provide a potential therapeutic target in the treatment of PDAC. PMID: 28377226
  17. AQP3 was upregulated, and AQP7 and AQP9 were downregulated in hepatocellular carcinoma. A high expression of AQP3 and low expression of AQP7 was significantly associated with the aggressive features of hepatocellular carcinoma. PMID: 27121567
  18. AQP3 inhibition that induced glycerol uptake reduction and glycerol administration would rehabilitate the cell proliferation. PMID: 26768614
  19. AQP3 increases in hepatocellular carcinoma. Auphen reduces AQP3 levels, suppressing tumor growth. PMID: 27022216
  20. roles of AQP-3 in AQP-3 aquaglyceroporin and ClC-3 chloride channels complex PMID: 26794461
  21. treatment of human HepG2 cells with TCDD also increased the expression of AQP3 mRNA and protein PMID: 26454884
  22. AQP3 knockdown retards the growth of NSCLC cells partially through inhibiting HIF-1alpha/VEGF and Raf/MEK/ERK signalling pathways. PMID: 26765123
  23. AQP3 was found to be expressed in 67.71% of parts with GIM, and AQP3 immunoreactivity was identified more frequently in severe GIM areas (P < 0.001). In short, the incidence and severity of GIM correlated with the distance from GC, and AQP3 was differentially expressed in goblet cells, with most AQP3-positive goblet cells presenting in severe GIM. PMID: 26506416
  24. AQP3 was expressed in trophoblast explants. Blocking it with CuSO4 prevented apoptosis. PMID: 26568619
  25. Expression of CXCL4 and aquaporin 3 and 10 mRNAs in middle ear effusion is associated with the pathophysiology of otitis media with effusion. PMID: 26810286
  26. results demonstrate that AQP3 is required for CXCL12-induced breast cancer cell signaling and directional migration by a mechanism involving the CXCL12-induced generation of extracellular H2O2 and subsequent intracellular transport by AQP3 PMID: 26830227
  27. We, for the first time, identified an ERE in the promoter of AQP3 gene, and found that estrogen might promote breast cancer development through activating ERE in the promoter of AQP3 gene and upregulating AQP3 expression in ER-positive breast cancer. PMID: 26219409
  28. these findings suggest that AQP3 is required for EGF-EGFR cell signaling in cancer cells and is a therapeutic target for cancer progression. PMID: 26896765
  29. AQP3 may play a role in nonmelanoma skin cancer pathogenesis PMID: 26107428
  30. the human aquaglyceroporins, i.e., AQP3, AQP7, AQP9 and AQP10 can act as silicon transporters in both Xenopus laevis oocytes and HEK-293 cells. PMID: 26313002
  31. AQP3-facilitated H2O2 transport is required for NF-kappaB activation in keratinocytes in the development of psoriasis. PMID: 26100668
  32. AQP3 protein expression is regulated by cAMP-PKA-CREB signal pathway. PMID: 25961543
  33. The results suggest that morphine increases the AQP3 expression level in the colon, which promotes water absorption from the luminal side to the vascular side and causes constipation. PMID: 25766885
  34. Report down-regulation of aquaporin3 expression by lipopolysaccharide via p38/c-Jun N-terminal kinase signalling pathway in HT-29 human colon epithelial cells. PMID: 25914463
  35. AQP-3 expression is increased in the burn wound following injury. PMID: 25603981
  36. Oleic acid-induced hepatic steatosis in HepG2 cells is associated with the coordinated regulation of aquaporin 3 and aquaporin 9 via activation of p38 signaling PMID: 25105540
  37. There is a correlation between AQP3 protein expression and tumour stage and grade in urothelial cancer, with AQP3 expression being reduced or lost in tumours of higher grade and stage. PMID: 24022233
  38. AQP3 induces ERK1/2 activation, thereby increasing MMP3 expression and secretion, which in turn promotes prostate cancer cell motility and invasion. PMID: 25515250
  39. The opening of ClC-3 transports Cl(-) across the cell membrane and then drives the efflux of water through AQP-3 channels and ion channels; AQP-3 may interact with ClC-3 in order to regulate the effluxes of chloride and water. PMID: 25450461
  40. AQP3 expression in tumor tissue may be considered as a potential prognostic marker in patients with HER2-over-expressing EBC after curative surgery. PMID: 25964557
  41. results provide a mechanism by which AQP3 is upregulated, as well as highlight the importance of miR-874 in gastric cancer development and progression. PMID: 23800944
  42. Resveratrol inhibited NHEK proliferation by downregulating the expression of AQP3 in an SIRT1/ARNT/ERK dependent fashion. PMID: 24726500
  43. Investigated both AQP3 expression in the skin of an experimental burn model. Observations suggest that dermal AQP3 gene expression was increased to maintain water homeostasis in response to dehydration from burn. PMID: 24508472
  44. We conclude that water fluxes accompanying CFTR activity are linked to AQP3 but not to a cAMP-stimulated aqueous pore in the CFTR protein PMID: 24338365
  45. AQP3 promotes epithelial-mesenchymal transition in human cases of gastric adenocarcinoma. PMID: 24887009
  46. analysis of the molecular link between the circadian clock and AQP3 function in mouse dorsal skin and HaCaT cells PMID: 24418925
  47. AQP3-Notch1 axis may provide insight into epidermal homeostasis control. PMID: 24260356
  48. Our data suggest for the first time that the aberrant expression of AQP3 and AQP5 proteins may be strongly related to tumor progression and prognosis in patients with hepatocellular carcinoma PMID: 24224160
  49. AQP3-EGFP diffusion is altered upon increased cAMP, which may correspond to basolateral adaptations in response to the increased apical water readsorption. PMID: 24452376
  50. High expression of both AQP3 and AQP5, not each alone, is an independent poor prognostic factor in ESCC patients. PMID: 23797770
  51. AQP3 is an endogenous suppressor of cell apoptosis in skin fibroblasts through up-regulation of Bcl-2. PMID: 23463292
  52. our results suggest AQP3 overexpression in human colorectal carcinoma cells could facilitate cell migration. PMID: 23165320
  53. Data suggest that assessment of aquaporin (AQP) 3 protein expression could be used to help stratify the behavior of patients with pT1 urothelial bladder cancer. PMID: 23043286
  54. Data suggget that the aquaglyceroporin AQP3 is required for cytotoxic activity of 5'-DFUR and gemcitabine in the breast cancer cell line MCF7 and the colon adenocarcinoma cell line HT29. PMID: 23017148
  55. The present study found that AQP3 expression correlated with H. pylori infection status in gastric cancer tissues PMID: 23152856
  56. TGF-beta1 induces AQP3 and that AQP3 has a critical role in TGF-beta-induced human peritoneal mesothelial cell migration. PMID: 23041062
  57. AQP3 expression significantly increased in advanced stage of cervical cancer, deeper infiltration, metastatic lymph nodes and larger tumor volume. PMID: 22901156
  58. The differential expressions of AQP3 and AQP4 may reflect the biological nature and/or function of normal, hyperplastic, and neoplastic thyroid cells and additionally may have value in determining differential diagnoses of thyroid tumors. PMID: 22808259
  59. AQP3 expression was closely correlated with microvascular density in NSCLC PMID: 22814259
  60. AQP3 is transcriptionally activated in retinal pigment epithelial (RPE) cells by various pathogenic factors involved in the development of retinal edema in situ. PMID: 22535323
  61. Potent inhibition of aquaglyceroporin-3 by a gold-based compound, through the ability of Au(III) to interact with the sulphydryl groups of proteins. PMID: 22624030
  62. Overexpression of both AQP5 and AQP3 were immunohistochemically observed on tumor cells in squamous cell carcinoma PMID: 22576684
  63. This is the first description of a potential role for aquaporin 3 in bladder cancer. PMID: 22197473
  64. Kanglaite inhibited UVB-induced AQP3 down-regulation of cultured human skin keratinocytes. PMID: 22211241
  65. The gene expressions of AQP3 and AQP4 in gastric mucosa are different in patients with various degrees of spleen-stomach dampness-heat syndrome of chronic superficial gastritis. PMID: 20141731
  66. PPAR and LXR activators stimulate AQP3 expression, providing an additional mechanism by which PPAR and LXR activators regulate epidermal function. PMID: 21457357
  67. Data not only describe the correlation between transcellular and paracellular pathways in human intestines, but also show that targeted knockdown of aquaporin 3 might impair the intestinal barrier integrity. PMID: 21907710
  68. aquaporin 3 is an essential membrane pathway for sperm regulatory volume decrease that balances the "trade-off" between sperm motility and cell swelling upon physiological hypotonicity PMID: 21135872
  69. overexpression of AQP3 was seen primary squamous cell carcinomas (SCC) such as esophageal and lingual cancers and cultured SCC cell lines; suppression of AQP3 protein or function caused cell death in SCC cell lines by inhibition of cell adhesion PMID: 21401805
  70. involvement of AQP3 in epidermal hyperplasia by a mechanism involving upregulated AQP3 expression and consequent enhancement of keratinocyte proliferation PMID: 21191421
  71. REVIEW: evidence supporting a role for AQP3 in the epidermis PMID: 21276418
  72. One sequence alteration, homozygous c.105G->C in aquaporin 3 was detected in 11 out of 34 patients but not in 100 control chromosomes. PMID: 21063116
  73. The authors conclude that AQP3 plays a critical role in human epidermal growth factor (hEGF) induced cell migration and proliferation in human gastric cancer. PMID: 20364107
  74. AQP3 decreased with increasing age in normal human skin, normal human epidermal keratinocytes, and skin fibroblast samples. PMID: 20546216
  75. Found AQP3 is involved in wound healing in human skin keratinocytes. Trans-Zeatin pretreatment also attenuates UV-induced decreased water permeability in HaCaT cells. PMID: 20596606
  76. reduced AQP3 in vitiliginous keratinocytes might be responsible for their reduced survival PMID: 20428189
  77. AQP3, 4 & 5 exhibited differential expression between human gastric carcinoma & corresponding normal tissue;AQP3 & AQP5 expression were associated with lymph node metastasis & lymphovascular invasion PMID: 20106632
  78. Mucinous colorectal adenocarcinomas displayed 182 upregulated and 135 downregulated genes. The most upregulated genes included those involved in cellular differentiation and mucin metabolism (eg, AQP3). PMID: 20485009
  79. Up-regulated expression of AQP3 in ascending colon and down-regulated expression of AQP9 in descending colon are presented in patients with functional constipation as compared to patients without functional constipation. PMID: 18197497
  80. we analyzed the oligomerization of the AQP3 aquaglyceroporin, which presents a mixed selectivity for water, glycerol, and urea. PMID: 11751877
  81. the role of aquaporin-3 is to water-clamp viable layers of the epidermis in order to improve the hydration of the epidermis below the stratum corneum. PMID: 11918716
  82. Aquaporin-3 protein was detected in the human peritoneal tissue and is upregulated by glucose PMID: 12234316
  83. first reported cases of AQP3 deficiency in humans and provide the molecular basis of a new blood group system, GIL, encoded by the AQP3 protein PMID: 12239222
  84. analysis of effect of nickel and extracellular acidification on water permeability of human aquaporin-3 in lung epithelial cells PMID: 12773542
  85. results indicate that ANP and BNP up-regulated the expression of AQP3 mRNA and protein, and both PK-A and PK-G dependent pathways mediated this effect PMID: 15375592
  86. The distribution and expression levels of AQP3 in normal human tissue. PMID: 15703994
  87. analysis of AQP3 expression in human fetal airway epithelial progenitor cells PMID: 16043462
  88. AQP3 water channels serve as an essential pathway for volume-regulatory water transport in human epithelial cells. PMID: 16596446
  89. provides evidence of AQP3 expression in human fetal membranes and demonstrates that AQP3 expression in primary human amnion cell culture is up-regulated by second-messenger cAMP PMID: 16638588
  90. lower expression of AQP3 and enhanced expression of MRP2 are responsible for lower arsenic accumulation in arsenic-resistant cells PMID: 16672223
  91. Increased expression and altered cellular distribution of AQP3 is found in atopic eczema, and this may contribute to water loss. PMID: 16918518
  92. Lung carcinomas, especially adenocarcinomas, can produce AQP3, possibly in connection with their functional and/or biological nature. PMID: 17056099
  93. results suggest that Foxa2 is one of the transcriptional regulators for AQP3 gene expression regulated by insulin PMID: 17471492
  94. all trans retinoic acid increased AQP3 expression and enhanced biological activity in human skin PMID: 17943189
  95. Provide evidence for involvement of AQP3-facilitated water transport in epidermal cell migration and for AQP3-facilitated glycerol transport in epidermal cell proliferation. PMID: 17968524
  96. The results suggest that water-channel protein may be involved in the preservation of cellular character in the human prostate and prostate cancer is associated with an alteration of water-transporting mechanisms. PMID: 18036046
  97. The data suggest potential roles of AQP3 and 5 in the regulation of airway hypersecretion, perhaps ultimately providing a target for treating such diseases PMID: 18280225
  98. Although reticulocytes are highly abundant in malarial anemia, low AQP3 expression was observed PMID: 18435676
  99. Discuss the role of AQP3 in TNF-alpha-mediated inflammation and the development of periodontitis. PMID: 18543247
  100. The trafficking of AQP3 evoked by epinephrine is mediated by protein kinase C in Caco-2 cells. PMID: 18601899

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Subcellular Location Basolateral cell membrane, Multi-pass membrane protein
Protein Families MIP/aquaporin (TC 1.A.8) family
Tissue Specificity Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
Database Links

HGNC: 636

OMIM: 600170

KEGG: hsa:360

STRING: 9606.ENSP00000297991

UniGene: Hs.234642

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