Product Details

Target Name

BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)

Alternative Names

BAMBI ELISA Kit; BAMBI_HUMAN ELISA Kit; BMP and activin membrane bound inhibitor homolog ELISA Kit; BMP and activin membrane-bound inhibitor homolog ELISA Kit; NMA ELISA Kit; Non metastatic gene A protein ELISA Kit; Non-metastatic gene A protein ELISA Kit; Putative transmembrane protein NMA ELISA Kit

Abbreviation

BAMBI

Uniprot No.

Q13145

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BAMBI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-105
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-99
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of human BAMBI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	97	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.324	2.495	2.410	2.286
5	1.551	1.630	1.591	1.467
2.5	0.950	0.904	0.927	0.803
1.25	0.572	0.542	0.557	0.433
0.625	0.363	0.379	0.371	0.247
0.312	0.264	0.283	0.274	0.150
0.156	0.224	0.216	0.220	0.096
0	0.127	0.121	0.124

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human BAMBI ELISA Kit was designed for the quantitative measurement of Human BAMBI protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Background

Function
(From Uniprot)

Negatively regulates TGF-beta signaling.

Gene References into Functions

BAMBI promotes macrophage proliferation and differentiation in gliomas. PMID: 29286116
These results showed that BMP2 activated SMAD1/5/8 phosphorylation and up-regulated BAMBI mRNA in human granulosa-lutein cells. PMID: 28578012
Data show that BAMBI is expressed in both circulating CD4+ T cells and plasma and may be characterized as a novel immune regulator for COPD. Strikingly, BAMBI expression was significantly stronger in COPD patients and displayed strong correlations with enhanced plasma TGF-beta 1 levels and the Th17/Treg ratio. PMID: 27549738
Lipopolysaccharides-mediated TLR4 signaling enhances TGF-beta response through downregulating BAMBI during prostatic hyperplasia. PMID: 27243216
Low BAMB1 expression is associated with Non-Small Cell Lung Cancer. PMID: 27197161
TGFbeta target genes including TGFBI, BAMBI, COL3A1 and SERPINE1 are significantly increased in Diamond Blackfan Anemia induced pluripotent stem cells PMID: 26258650
The observations illuminated that BAMBI inhibits adipogenesis by a feedback loop (BAMBI-->beta-catenin nuclear translocation-->BAMBI), which forms with Wnt/beta-catenin signaling. PMID: 24798646
betacatenin expression was downregulated as a result of knocking down of BAMBI, and TGF-beta was downregulated in a similar manner. PMID: 24912656
BAMBI alone or in combination with Smad7 was significantly associated with the OS and DFS. These findings suggest that BAMBI and Smad7 may cooperatively inhibit the TGF-beta signaling, and thus promote the progression of gastric cancer. PMID: 24752577
study demonstrates that LPS- and TNF-alpha-induced NF-kappaBp50-HDAC1 interaction represses BAMBI transcriptional activity, which contributes to TLR4-mediated enhancement of TGF-beta signaling in hepatic stellate cells during liver fibrosis. PMID: 24448807
Overexpression of BAMBI is associated with the growth and invasion of human osteosarcoma. PMID: 23807684
Metformin-mediated Bambi expression in hepatic stellate cells induces prosurvival Wnt/beta-catenin signaling PMID: 22406377
Alterations in BAMBI may play a role in the (patho)physiology of obesity, and manipulation of BAMBI may present a novel therapeutic approach to improve adipose tissue function. PMID: 22187378
Data show that hepatoprotective effects of adiponectin include induction of BAMBI which is reduced in human fatty liver. PMID: 21496456
BAMBI protein is expressed in endothelial cells and is regulated by autophagy and lysosomal degradation. PMID: 20886049
Expression of the TGF pseudoreceptor BAMBI in the human lung, which is upregulated in response to nontypeable Haemophilus influenzae infection in COPD lung tissue in vivo and in vitro. PMID: 20513241
Authors demonstrate for the first time a nuclear co-translocation of BAMBI with Smad2/3 upon TGF-geta treatment. Moreover, overexpression of BAMBI in an in vitro model led to significantly increased proliferation. PMID: 20189233
beta-catenin interferes with transforming growth factor-beta-mediated growth arrest by inducing the expression of BAMBI PMID: 14660579
Our findings suggest that BAMBI transcription is regulated by TGF-beta signaling through direct binding of SMAD3 and SMAD4 to the BAMBI promoter. PMID: 15240101
BAMBI was linked to a potentially aggressive tumor phenotype and predicted tumor recurrence and cancer-related death in CRC. PMID: 18756595
BAMBI interacts with Wnt receptor Frizzled5, coreceptor LRP6, and Dishevelled2 and increases the interaction between Frizzled5 and Dishevelled2 PMID: 18838381
hypermethylation of the BAMBI gene promoter that leads to BAMBI gene suppression may be one of the epigenetic events affecting the invasiveness or aggressiveness of bladder cancers PMID: 19326429
A colorectal cancer expression profile that includes transforming growth factor beta inhibitor BAMBI predicts metastatic potential. PMID: 19328798
Mechanistically, in addition to interfering with the complex formation between the type I and type II receptors, hBAMBI cooperates with Smad7 to inhibit TGF-beta signaling PMID: 19758997

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

BAMBI family

Tissue Specificity

High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested.

Database Links

HGNC: 30251

OMIM: 604444

KEGG: hsa:25805

STRING: 9606.ENSP00000364683

UniGene: Hs.533336

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Human BMP and activin membrane-bound inhibitor homolog(BAMBI) ELISA kit

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