Human BMP and activin membrane-bound inhibitor homolog(BAMBI) ELISA kit

Instructions
Code CSB-EL002549HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)
Alternative Names NMA, BMP and activin membrane-bound inhibitor|non-metastatic gene A protein|putative transmembrane protein
Abbreviation BAMBI
Uniprot No. Q13145
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BAMBI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 100
Range % 95-105
1:2 Average % 84
Range % 80-88
1:4 Average % 95
Range % 90-99
1:8 Average % 93
Range % 86-98
Recovery
The recovery of human BAMBI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 97 92-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.324 2.495 2.410 2.286
5 1.551 1.630 1.591 1.467
2.5 0.950 0.904 0.927 0.803
1.25 0.572 0.542 0.557 0.433
0.625 0.363 0.379 0.371 0.247
0.312 0.264 0.283 0.274 0.150
0.156 0.224 0.216 0.220 0.096
0 0.127 0.121 0.124  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Negatively regulates TGF-beta signaling.
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families BAMBI family
Tissue Specificity High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested.
Database Links

HGNC: 30251

OMIM: 604444

KEGG: hsa:25805

STRING: 9606.ENSP00000364683

UniGene: Hs.533336

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