Human CD5 Antigen-like(CD5L) ELISA Kit

Code CSB-E13423h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CD5L ELISA Kit was designed for the quantitative measurement of Human CD5L protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

Target Name CD5 molecule-like
Alternative Names AAC-11 ELISA Kit; AIM ELISA Kit; API6 ELISA Kit; APOPTOSIS INHIBITOR 6 ELISA Kit; APOPTOSIS INHIBITOR OF MACROPHAGES ELISA Kit; CD5 antigen like (scavenger receptor cysteine rich family) ELISA Kit; CD5 antigen-like ELISA Kit; Cd5l ELISA Kit; CD5L_HUMAN ELISA Kit; CT 2 ELISA Kit; CT-2 ELISA Kit; Highly similar to ANTIGEN WC1.1 [Bos taurus] ELISA Kit; IgM associated peptide ELISA Kit; IgM-associated peptide ELISA Kit; Pdp ELISA Kit; PRO229 ELISA Kit; SCAVENGER RECEPTOR CYSTEINE-RICH FAMILY ELISA Kit; SP ALPHA ELISA Kit; SP-alpha ELISA Kit; Spalpha ELISA Kit
Abbreviation CD5L
Uniprot No. O43866
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD5L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %84-92
1:2Average %103
Range %96-107
1:4Average %92
Range %87-94
1:8Average %101
Range %97-104
Recovery
The recovery of human CD5L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9793-101
EDTA plasma (n=4)9387-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
4002.335 2.245 2.290 2.135
2001.474 1.577 1.526 1.371
1001.017 0.975 0.996 0.841
500.591 0.602 0.597 0.442
250.386 0.397 0.392 0.237
12.50.278 0.285 0.282 0.127
6.250.221 0.225 0.223 0.068
00.154 0.156 0.155
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies. Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis. Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy.
Gene References into Functions
  1. Taken together with our results on the aberrant ex vivo production of CD5L, this study has provided new evidence for the potential immunopathological role of CD5L in the exacerbation of SLE disease, and circulating concentration of CD5L may act as a potential disease marker for the monitoring of disease severity and therapeutic effects in SLE patients. PMID: 30173083
  2. AIM enhances intratubular debris clearance and ameliorates acute kidney injury via interaction with KIM-1. PMID: 26726878
  3. The present study provides the first evidence that apoptosis inhibitor of macrophages binds to IGFBP2, 3 and 4. PMID: 26135353
  4. CD36 is involved in autophagy; the CD5L-CD36 axis has a role in the induction of macrophage autophagy PMID: 25713983
  5. CD5L is a key protein in the control of immune homeostasis and inflammatory disease. (Review) PMID: 26048980
  6. Apoptosis inhibitor of macrophage levels were markedly higher in patients with advanced liver damage, regardless of disease type, and correlated significantly with multiple parameters representing liver function. PMID: 25302503
  7. AIM contributes to key macrophage responses to M. tuberculosis PMID: 24223991
  8. Our data represent the first evidence that human AIM is involved in macrophage survival, adhesion, and foam cell formation PMID: 24295828
  9. N-glycosylation plays important roles in the secretion and lipolytic function of AIM PMID: 23236605
  10. AIM is involved in the progression of MetS in both an advancing and inhibitory fashion. Regulation of AIM could therefore be therapeutically applicable for MetS. PMID: 21970839
  11. Spalpha is an active constituent of the innate immune response of the host PMID: 16030018
  12. CD5L and ApoE were significantly up-regulated or down-regulated, respectively, in the plasma from AD patients compared with that from healthy donors. PMID: 19116453
  13. Dysregulation of myeloid cell populations by extracellular transgene Api6 signaling leads to abnormal myelopoiesis and lung cancer in bitransgenic mice. PMID: 19155514

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Subcellular Location Secreted. Cytoplasm.
Tissue Specificity Expressed in spleen, lymph node, thymus, bone marrow, and fetal liver, but not in non-lymphoid tissues.
Database Links

HGNC: 1690

OMIM: 602592

KEGG: hsa:922

STRING: 9606.ENSP00000357156

UniGene: Hs.134035

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