Code | CSB-E13423h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human CD5L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 84-92 | |||||
1:2 | Average % | 103 | ||||
Range % | 96-107 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-94 | |||||
1:8 | Average % | 101 | ||||
Range % | 97-104 |
The recovery of human CD5L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 93-101 | ||||
EDTA plasma (n=4) | 93 | 87-97 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E13423h is a sandwich ELISA kit developed to measure concentrations of human CD5L in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CD5L in samples. The color intensity is measured at 450 nm via a microplate reader.
CD5L is a secreted protein produced mostly by macrophages in lymphoid and inflamed tissues. It plays a critical role in immune homeostasis and inflammatory disease. It is involved in various diseases such as lipid metabolic disease, hepatocellular carcinoma, fungus-induced peritonitis, acute kidney injury, and myocardial infarction. CD5L localizes to CD36 to promote the transcription of genes involved in the regulation of mitochondrial biogenesis to maintain energy and metabolic homeostasis. CD5L also activates autophagy in macrophages while altering inflammatory phenotypes, specifically reducing TNF and IL-1 beta expression while elevating the immunosuppressive cytokine IL-10. Serum or plasma CD5L levels are increased in inflammatory conditions including liver cirrhosis, chronic hepatitis C, atopic dermatitis, and Kawasaki disease.
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