Human Cartilage intermediate layer protein 1(CILP) ELISA kit

Instructions
Code CSB-EL005437HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
Alternative Names Cartilage intermediate layer protein 1 ELISA Kit; Cartilage intermediate layer protein 1 C1 ELISA Kit; Cartilage intermediate layer protein 1 C2 ELISA Kit; cartilage intermediate layer protein ELISA Kit; cartilage intermediate layer protein; nucleotide pyrophosphohydrolase ELISA Kit; Cartilage intermediate-layer protein ELISA Kit; CILP 1 ELISA Kit; CILP ELISA Kit; CILP-1 ELISA Kit; CILP1_HUMAN ELISA Kit; HGNC:1980 ELISA Kit; HsT18872 ELISA Kit
Abbreviation CILP
Uniprot No. O75339
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.4 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CILP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %94
Range %90-98
1:10Average %105
Range %100-112
1:20Average %84
Range %80-88
1:40Average %95
Range %90-100
Recovery
The recovery of human CILP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-98
EDTA plasma (n=4)9894-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
60002.098 2.201 2.150 2.027
30001.512 1.454 1.483 1.360
15000.983 0.934 0.959 0.836
7500.611 0.643 0.627 0.504
3750.330 0.338 0.334 0.211
187.50.282 0.302 0.292 0.169
93.750.192 0.207 0.200 0.077
00.125 0.120 0.123
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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 Q&A
Q:

what part of the protein this ELISA kit targets? As I am aware,
the Cilp gene encodes a precursor that is cleaved into two fragments.
I was curious as to whether this kit targets a specific fragment.

A:
Thanks for your inquiry!
CSB-EL005437HU
This kit is designed according to: https://www.uniprot.org/uniprot/O75339.
It detects CILP protein. The two fragments you mentioned should be C1 and C2. Sorry we are not clear if these 2 forms can be detected out or not. If the fragment is within the antibody's recognition area, then it can be detected out. However, in general the protein has spatial structure, so it is difficult to predict if it can be detected out or not. We suggest you do a pretest first.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.
Gene References into Functions
  1. CILP rs2073711 TT is associated with increased risk of symmetrical hand osteoarthritis particularly in individuals with low variation in work tasks. PMID: 29233086
  2. Meta-analysis. Our results confirm the positive association between CILP and intervertebral disc degeneration, providing novel clues for clarifying the role of CILP in the development of IVD. PMID: 27359356
  3. CILP is involved in the etiology of intervertebral disc degeneration among young adults. PMID: 22107760
  4. The researchers found that the single nucleotide polymorphism (1184T/C) of the CILP gene is associated an increased risk of lumbar disc degeneration in male athletes. PMID: 20724643
  5. CILP regulates TGF-beta signaling and that this regulation has a crucial role in the etiology and pathogenesis of LDD. PMID: 15864306
  6. These observations, together with the finding that CILP protein binds and inhibits TGF-beta1, suggest that CILP and TGF-beta1 may form a functional feedback loop that controls chondrocyte metabolism. PMID: 16413503
  7. SNP analysis suggested that the CILP gene is not a major risk factor for symptoms of lumbar disc disease in Finnish or Chinese populations. PMID: 17220213
  8. the CILP gene 1184T/C polymorphism is a significant risk factor for lumbar disc degeneration occurrence in Japanese collegiate judo athletes PMID: 19569011

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Involvement in disease Intervertebral disc disease (IDD)
Subcellular Location Secreted, extracellular space, extracellular matrix
Tissue Specificity Specifically expressed in cartilage. Localizes in the intermediates layer of articular cartilage but neither in the superficial nor in the deepest regions. Specifically and highly expressed in intervertebral disk tissue. Expression increases with aging in
Database Links

HGNC: 1980

OMIM: 603489

KEGG: hsa:8483

STRING: 9606.ENSP00000261883

UniGene: Hs.442180

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