Name: Human Cartilage intermediate layer protein 1(CILP) ELISA kit
Brand: CUSABIO
SKU: CSB-EL005437HU

Product Details

Target Name

cartilage intermediate layer protein, nucleotide pyrophosphohydrolase

Alternative Names

Cartilage intermediate layer protein 1 ELISA Kit; Cartilage intermediate layer protein 1 C1 ELISA Kit; Cartilage intermediate layer protein 1 C2 ELISA Kit; cartilage intermediate layer protein ELISA Kit; cartilage intermediate layer protein; nucleotide pyrophosphohydrolase ELISA Kit; Cartilage intermediate-layer protein ELISA Kit; CILP 1 ELISA Kit; CILP ELISA Kit; CILP-1 ELISA Kit; CILP1_HUMAN ELISA Kit; HGNC:1980 ELISA Kit; HsT18872 ELISA Kit

Abbreviation

CILP

Uniprot No.

O75339

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

93.75 pg/mL-6000 pg/mL

Sensitivity

23.4 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CILP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	90-98
1:10	Average %	105
	Range %	100-112
1:20	Average %	84
	Range %	80-88
1:40	Average %	95
	Range %	90-100

Recovery

The recovery of human CILP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	98	94-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.098	2.201	2.150	2.027
3000	1.512	1.454	1.483	1.360
1500	0.983	0.934	0.959	0.836
750	0.611	0.643	0.627	0.504
375	0.330	0.338	0.334	0.211
187.5	0.282	0.302	0.292	0.169
93.75	0.192	0.207	0.200	0.077
0	0.125	0.120	0.123

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

Cartilage intermediate layer protein 1 (CILP) is a secreted glycoprotein found primarily in the intermediate layer of articular cartilage. This protein functions as a nucleotide pyrophosphohydrolase and plays an important role in cartilage matrix organization and maintenance. CILP has been linked to various cartilage-related disorders and osteoarthritis pathogenesis, making it a significant biomarker for joint health research. Its expression levels can offer insights into cartilage metabolism and degenerative processes affecting joint function.

The Human Cartilage intermediate layer protein 1 ELISA kit (CSB-EL005437HU) uses a quantitative sandwich measurement principle for detecting CILP in human samples. This assay works with serum, plasma, and tissue homogenates with a detection range of 93.75 pg/mL to 6000 pg/mL and shows a sensitivity of 23.4 pg/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in cardiovascular disease research to measure circulating cartilage intermediate layer protein levels in patient populations. Studies have applied the kit to examine biomarker associations in heart failure patients and to compare protein concentrations between different clinical groups. The kit has also been used in dermatological research examining hidradenitis suppurativa patients.

• Cardiovascular research: Measuring circulating biomarker levels in heart failure patients with reduced ejection fraction and comparison with control populations
• Clinical biomarker studies: Examining protein concentration differences between patient groups and healthy individuals in cardiovascular disease contexts
• Dermatological research: Measuring serum protein levels in hidradenitis suppurativa patients to explore disease-associated biomarkers
• Comparative assay validation: Testing kit performance alongside other commercial immunoassays to establish measurement correlations and analytical reliability

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Customer Reviews and Q&A

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■ Q&A

what part of the protein this ELISA kit targets? As I am aware,
the Cilp gene encodes a precursor that is cleaved into two fragments.
I was curious as to whether this kit targets a specific fragment.

Thanks for your inquiry!
CSB-EL005437HU
This kit is designed according to: https://www.uniprot.org/uniprot/O75339.
It detects CILP protein. The two fragments you mentioned should be C1 and C2. Sorry we are not clear if these 2 forms can be detected out or not. If the fragment is within the antibody's recognition area, then it can be detected out. However, in general the protein has spatial structure, so it is difficult to predict if it can be detected out or not. We suggest you do a pretest first.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.

Gene References into Functions

CILP rs2073711 TT is associated with increased risk of symmetrical hand osteoarthritis particularly in individuals with low variation in work tasks. PMID: 29233086
Meta-analysis. Our results confirm the positive association between CILP and intervertebral disc degeneration, providing novel clues for clarifying the role of CILP in the development of IVD. PMID: 27359356
CILP is involved in the etiology of intervertebral disc degeneration among young adults. PMID: 22107760
The researchers found that the single nucleotide polymorphism (1184T/C) of the CILP gene is associated an increased risk of lumbar disc degeneration in male athletes. PMID: 20724643
CILP regulates TGF-beta signaling and that this regulation has a crucial role in the etiology and pathogenesis of LDD. PMID: 15864306
These observations, together with the finding that CILP protein binds and inhibits TGF-beta1, suggest that CILP and TGF-beta1 may form a functional feedback loop that controls chondrocyte metabolism. PMID: 16413503
SNP analysis suggested that the CILP gene is not a major risk factor for symptoms of lumbar disc disease in Finnish or Chinese populations. PMID: 17220213
the CILP gene 1184T/C polymorphism is a significant risk factor for lumbar disc degeneration occurrence in Japanese collegiate judo athletes PMID: 19569011

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Involvement in disease

Intervertebral disc disease (IDD)

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Specifically expressed in cartilage. Localizes in the intermediates layer of articular cartilage but neither in the superficial nor in the deepest regions. Specifically and highly expressed in intervertebral disk tissue. Expression increases with aging in

Database Links

HGNC: 1980

OMIM: 603489

KEGG: hsa:8483

STRING: 9606.ENSP00000261883

UniGene: Hs.442180

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Code	CSB-EL005437HU
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.