Human Collectin-12(COLEC12) ELISA kit

Instructions
Code CSB-EL005762HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name collectin sub-family member 12
Alternative Names CL P1 ELISA Kit; CL-P1 ELISA Kit; CLP1 ELISA Kit; COL12_HUMAN ELISA Kit; COLEC 12 ELISA Kit; colec12 ELISA Kit; Collectin placenta 1 ELISA Kit; Collectin placenta protein 1 ELISA Kit; Collectin sub family member 12 ELISA Kit; Collectin-12 ELISA Kit; hCL P1 ELISA Kit; hCL-P1 ELISA Kit; NSR2 ELISA Kit; Nurse cell scavenger receptor 2 ELISA Kit; SCARA4 ELISA Kit; Scavenger receptor class A member 4 ELISA Kit; Scavenger receptor with C type lectin ELISA Kit; Scavenger receptor with C-type lectin ELISA Kit; SRCL ELISA Kit
Abbreviation COLEC12
Uniprot No. Q5KU26
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-88
1:2 Average % 85
Range % 82-89
1:4 Average % 91
Range % 86-95
1:8 Average % 104
Range % 101-107
Recovery
The recovery of human COLEC12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-93
EDTA plasma (n=4) 90 85-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.541 2.698 2.620 2.498
10 2.039 2.000 2.020 1.898
5 1.307 1.368 1.338 1.216
2.5 0.717 0.738 0.728 0.606
1.25 0.402 0.398 0.400 0.278
0.625 0.281 0.278 0.280 0.158
0.312 0.183 0.179 0.181 0.059
0 0.123 0.120 0.122  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). May also play a role in the clearance of amyloid-beta in Alzheimer disease.
Gene References into Functions
  1. CL-P1 is a novel receptor involved in myelin uptake by phagocytes and likely plays a role in multiple sclerosis lesion development. PMID: 28317919
  2. rs17684886 (ZNRF1) and rs599019 (COLEC12) are associated with diabetic retinopathy and rs6427247 (SCYL1BP1) and rs899036 (API5) are associated with severe diabetic retinopathy in Chinese patients with type 2 diabetes PMID: 25819896
  3. The common presence of Lewis(x) groups in granule protein glycans can thus target granule proteins for clearance by SRCL. PMID: 21561871
  4. The Le(x) residues of both, CEACAM1 and CEA, interact with the human Le(x)-binding glycan receptors DC-SIGN and SRCL. PMID: 20034698
  5. haplotype structure of the CL-P1 gene obtained from six single-nucleotide polymorphisms that were genotyped with 108 alleles in Japanese subjects PMID: 12601552
  6. SRCL is expressed in some but not all nurse-like cells; its C-type lectin domain binds specifically to several carbohydrates including GalNAc, T and Tn antigen in a Ca2+-dependent manner. PMID: 12761161
  7. SRCL might be involved in selective clearance of specific desialylated glycoproteins from circulation and/or interaction of cells bearing Lewis(x)-type structures with the vascular endothelium PMID: 15845541
  8. CL-P1 is not a common membrane protein on endothelial cells found in normal tissues under steady state conditions. PMID: 18423602
  9. CL-P1 predominantly mediated phagocytosis for fungi in vascular endothelia. PMID: 19073604

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Subcellular Location Membrane, Single-pass type II membrane protein
Tissue Specificity Expressed in perivascular macrophages. Expressed in plaques-surrounding reactive astrocytes and in perivascular astrocytes associated with cerebral amyloid angiopathy (CAA) in the temporal cortex of Alzheimer patient (at protein level). Strongly expressed
Database Links

HGNC: 16016

OMIM: 607621

KEGG: hsa:81035

STRING: 9606.ENSP00000383115

UniGene: Hs.464422

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