Product Details

Target Name

collectin sub-family member 12

Alternative Names

CL P1 ELISA Kit; CL-P1 ELISA Kit; CLP1 ELISA Kit; COL12_HUMAN ELISA Kit; COLEC 12 ELISA Kit; colec12 ELISA Kit; Collectin placenta 1 ELISA Kit; Collectin placenta protein 1 ELISA Kit; Collectin sub family member 12 ELISA Kit; Collectin-12 ELISA Kit; hCL P1 ELISA Kit; hCL-P1 ELISA Kit; NSR2 ELISA Kit; Nurse cell scavenger receptor 2 ELISA Kit; SCARA4 ELISA Kit; Scavenger receptor class A member 4 ELISA Kit; Scavenger receptor with C type lectin ELISA Kit; Scavenger receptor with C-type lectin ELISA Kit; SRCL ELISA Kit

Abbreviation

COLEC12

Uniprot No.

Q5KU26

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	85
	Range %	82-89
1:4	Average %	91
	Range %	86-95
1:8	Average %	104
	Range %	101-107

Recovery

The recovery of human COLEC12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	90	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.541	2.698	2.620	2.498
10	2.039	2.000	2.020	1.898
5	1.307	1.368	1.338	1.216
2.5	0.717	0.738	0.728	0.606
1.25	0.402	0.398	0.400	0.278
0.625	0.281	0.278	0.280	0.158
0.312	0.183	0.179	0.181	0.059
0	0.123	0.120	0.122

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human COLEC12 ELISA Kit was designed for the quantitative measurement of Human COLEC12 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Background

Function
(From Uniprot)

Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). May also play a role in the clearance of amyloid-beta in Alzheimer disease.

Gene References into Functions

CL-P1 is a novel receptor involved in myelin uptake by phagocytes and likely plays a role in multiple sclerosis lesion development. PMID: 28317919
rs17684886 (ZNRF1) and rs599019 (COLEC12) are associated with diabetic retinopathy and rs6427247 (SCYL1BP1) and rs899036 (API5) are associated with severe diabetic retinopathy in Chinese patients with type 2 diabetes PMID: 25819896
The common presence of Lewis(x) groups in granule protein glycans can thus target granule proteins for clearance by SRCL. PMID: 21561871
The Le(x) residues of both, CEACAM1 and CEA, interact with the human Le(x)-binding glycan receptors DC-SIGN and SRCL. PMID: 20034698
haplotype structure of the CL-P1 gene obtained from six single-nucleotide polymorphisms that were genotyped with 108 alleles in Japanese subjects PMID: 12601552
SRCL is expressed in some but not all nurse-like cells; its C-type lectin domain binds specifically to several carbohydrates including GalNAc, T and Tn antigen in a Ca2+-dependent manner. PMID: 12761161
SRCL might be involved in selective clearance of specific desialylated glycoproteins from circulation and/or interaction of cells bearing Lewis(x)-type structures with the vascular endothelium PMID: 15845541
CL-P1 is not a common membrane protein on endothelial cells found in normal tissues under steady state conditions. PMID: 18423602
CL-P1 predominantly mediated phagocytosis for fungi in vascular endothelia. PMID: 19073604

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Subcellular Location

Membrane; Single-pass type II membrane protein. Note=Forms clusters on the cell surface.

Tissue Specificity

Expressed in perivascular macrophages. Expressed in plaques-surrounding reactive astrocytes and in perivascular astrocytes associated with cerebral amyloid angiopathy (CAA) in the temporal cortex of Alzheimer patient (at protein level). Strongly expressed

Database Links

HGNC: 16016

OMIM: 607621

KEGG: hsa:81035

STRING: 9606.ENSP00000383115

UniGene: Hs.464422

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Human Collectin-12(COLEC12) ELISA kit

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