Human Complement C1q tumor necrosis factor-related protein 9A(C1QTNF9) ELISA kit

Code CSB-EL003654HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
C1q and tumor necrosis factor related protein 9
Alternative Names
C1QTNF9 ELISA kit; C1QTNF9A ELISA kit; UNQ6503/PRO21380Complement C1q and tumor necrosis factor-related protein 9A ELISA kit; Complement C1q and tumor necrosis factor-related protein 9 ELISA kit
Abbreviation
C1QTNF9
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C1QTNF9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %91-101
1:2Average %101
Range %95-104
1:4Average %92
Range %85-95
1:8Average %100
Range %94-103
Recovery
The recovery of human C1QTNF9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)9082-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.840 2.799 2.820 2.711
102.104 1.997 2.051 1.942
51.171 1.151 1.161 1.052
2.50.532 0.523 0.528 0.419
1.250.349 0.358 0.354 0.245
0.6250.233 0.242 0.238 0.129
0.3120.161 0.157 0.159 0.050
00.111 0.107 0.109
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human C1QTNF9 ELISA Kit was designed for the quantitative measurement of Human C1QTNF9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Function
(From Uniprot)
Probable adipokine. Activates AMPK, AKT, and p44/42 MAPK signaling pathways.
Gene References into Functions
  1. suggesting the protective potentials of C1q tumour necrosis factor-related protein 9 in the progression of peripheral arterial disease in human type 2 diabetes mellitus PMID: 29543038
  2. Authors demonstrate that CTRP9 regulates growth, differentiation, and apoptosis of HaCaT human keratinocytes. We found that CTRP9 augmented expression of transforming growth factor beta 1 (TGFbeta1) by transcription factor activator protein 1 (AP-1) binding activity and phosphorylation of p38 in a dose-dependent manner. PMID: 29145717
  3. CTRP9 inhibits the cholesterol-induced Vascular smooth muscle cell phenotype switch and cell dysfunction by activating PRKAA1. PMID: 28524645
  4. results revealed increased circulating levels of CTRP9 in T2DM and CAD individuals which suggests a compensatory response to insulin resistance, inflammatory milieu and endothelial dysfunction; however, more studies are needed to confirm this PMID: 29381773
  5. CTRP9 levels are elevated in obesity and significantly decrease following weight loss surgery. PMID: 26982010
  6. Plasma CTRP9 levels are associated with atherosclerosis in diabetic patients without CKD, independently of obesity, adiponectin, and traditional cardiovascular risk factors PMID: 28070523
  7. The up-regulation of CTRP9 during hypertrophic heart disease facilitates maladaptive cardiac remodeling and left ventricular dysfunction. PMID: 27821723
  8. Results demonstrate that CTRP9 alleviates hepatic steatosis through relief of endoplasmic reticulum stress via the AMPK-mediated induction of autophagy. PMID: 26419929
  9. Study demonstrates that CTRP9 attenuates cytokine-induced vascular inflammation in endothelial cells mediated by AMPK activation. PMID: 26523509
  10. Circulating and coronary CTRP9 plays an important role in the inflammation and coronary atherosclerosis of CAD patients. Serum CTRP9 is an independent protective factor of CAD PMID: 26457306
  11. Serum CTRP9 concentration was significantly and positively associated with arterial stiffness in patients with type 2 diabetes PMID: 25105737
  12. Serum CTRP9 concentrations were positively associated with favorable glucose or metabolic phenotypes and absence of metabolic syndrome, independent of serum total adiponectin concentrations. PMID: 24357853

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Subcellular Location
Secreted.
Tissue Specificity
Expressed predominantly in adipose tissue.
Database Links

HGNC: 28732

OMIM: 614285

KEGG: hsa:338872

STRING: 9606.ENSP00000333737

UniGene: Hs.362854

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